A differential proteome in tumors suppressed by an adenovirus‐based skin patch vaccine encoding human carcinoembryonic antigen

Abstract

We created an anti-tumor vaccine by using adenovirus as a vector which contains a cytomegalovirus early promoter-directed human carcinoembryonic antigen gene (AdCMVhCEA). In an attempt to develop the skin patch vaccine, we epicutaneously vaccinated Balb/c mice with AdCMV-hCPA. After nine weeks post-immunization, vaccinated mice evoked a robust antibody titer to CEA and demonstrated the capability of suppressing in vivo growth of implanted murine mammay adenocarioma cell line (JC-hCEA) tumor cells derived from a female Balb/c mouse. Proteomic analysis of the tumor masses in the non-vaccinated naïve and vaccinated mice reveal that six proteins change their abundance in the tumor mass. The levels of adenylate kinase 1, β-enolase, creatine kinase M chain, hemoglobin beta chain and prohibitin were statistically increased whereas the level of a creatine kinase fragment, which is undocumented, was decreased in the tumor of vaccinated mice. These proteins may provide a vital link between early-stage tumor suppression and immune response of skin patch vaccination.

Transcutaneous delivery of DNA/mRNA for cancer therapeutic vaccination

“…Regarding the biological methods, viral vectors are very efficient for delivering exogenous genes into host cells and exploiting the cellular machinery to facilitate the replication . For example, adenovirus and poxvirus based vaccines encoding human carcinoembryonic antigen (CEA) and epitope ovalbumin (OVA) 257–264 , respectively, were successfully applied onto the intact skin and injured skin for tumor immunotherapy. However, SC penetrability, vaccine specificity and the safety of viral vectors are still of concern.…”

A novel immunogenic spore coat-associated protein in Bacillus anthracis: Characterization via proteomics approaches and a vector-based vaccine system

“…Proteins in Dry-Strips were subsequently separated by 12.5% polyacrylamide gels in a Hoefer SE600 system (GE Healthcare Bio-Sciences Corp., Piscataway, NJ). Gels were visualized via silver nitrate staining [8]. In-gel digestion with trypsin and protein identification via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and quadrupole time-of-flight tandem mass spectrometry (Q-TOF MS/MS) analysis were performed essentially as described previously [9,10].…”

Intratumoral Decorin Gene Delivery by AAV Vector Inhibits Brain Glioblastomas and Prolongs Survival of Animals by Inducing Cell Differentiation

“…Proteomic analysis of global proteins in a cell can provide better insight into the underlying mechanisms of therapeutic treatments such as gene delivery by a viral vector as well as its therapeutic potentials [25,26]. Additionally, proteomics has been employed to study differentiation effects resulting from decorin expression in breast cancer cells [18].…”