A differential proteomic approach identifies structural and functional components that contribute to the differentiation of brain capillary endothelial cells

Pottiez, Gwënaël; Duban-Deweer, Sophie; Deracinois, Barbara; Gosselet, Fabien; Camoin, Luc; Hachani, Johan; Couraud, Pierre‐Olivier; Cecchelli, Roméo; Dehouck, Marie‐Pierre; Fénart, Laurence; Karamanos, Yannis; Flahaut, Christophe

doi:10.1016/j.jprot.2011.09.002

Cited by 24 publications

(21 citation statements)

References 39 publications

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“…As the peripheral actin ring breaks, stress fibers appear at the cell center, and contractile force exceeds the adhesion force . As the cell contracts, intercellular gaps increase in size and number, leading to vascular permeability . This study confirmed that HUVEC permeability was significantly increased due in part to F‐actin depolymerization in response to AGEs.…”

Section: Discussionsupporting

confidence: 72%

Advanced Glycation End Products Activate the miRNA/RhoA/ROCK2 Pathway in Endothelial Cells

Zeng

et al. 2014

Microcirculation

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Section: Discussionsupporting

confidence: 72%

Advanced Glycation End Products Activate the miRNA/RhoA/ROCK2 Pathway in Endothelial Cells

Zeng

et al. 2014

Microcirculation

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“…We have already reported quantitative changes in levels of actin-related proteins in BCECs displaying BBB features [21], [110].…”

Section: Discussionmentioning

confidence: 90%

TNAP and EHD1 Are Over-Expressed in Bovine Brain Capillary Endothelial Cells after the Re-Induction of Blood-Brain Barrier Properties

et al. 2012

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Although the physiological properties of the blood-brain barrier (BBB) are relatively well known, the phenotype of the component brain capillary endothelial cells (BCECs) has yet to be described in detail. Likewise, the molecular mechanisms that govern the establishment and maintenance of the BBB are largely unknown. Proteomics can be used to assess quantitative changes in protein levels and identify proteins involved in the molecular pathways responsible for cellular differentiation. Using the well-established in vitro BBB model developed in our laboratory, we performed a differential nano-LC MALDI-TOF/TOF-MS study of Triton X-100-soluble protein species from bovine BCECs displaying either limited BBB functions or BBB functions re-induced by glial cells. Due to the heterogeneity of the crude extract, we increased identification yields by applying a repeatable, reproducible fractionation process based on the proteins' relative hydrophobicity. We present proteomic and biochemical evidence to show that tissue non-specific alkaline phosphatase (TNAP) and Eps15 homology domain-containing protein 1(EDH1) are over-expressed by bovine BCECs after the re-induction of BBB properties. We discuss the impact of these findings on current knowledge of endothelial and BBB permeability.

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“…It was clear that apoPPF of low toxicity was suitable for constructing a novel carrier of nanometer drugs in order to study the apoptosis mechanism in various cancer cells, to advance the level of drug utilization, to decrease its toxicity in vivo, and to improve the efficiency of treating cancer [25]. A differential proteomic approach has been developed as an effective tool to identify the structure and functions of proteins [26]. In addition, shotgun proteomics is also used to find significant biomarkers [27,28].…”

Section: Discussionmentioning

confidence: 99%

Construction of nanometer cisplatin core-ferritin (NCC-F) and proteomic analysis of gastric cancer cell apoptosis induced with cisplatin released from the NCC-F

Huang

2012

Journal of Proteomics

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A differential proteomic approach identifies structural and functional components that contribute to the differentiation of brain capillary endothelial cells

Cited by 24 publications

References 39 publications

Advanced Glycation End Products Activate the miRNA/RhoA/ROCK2 Pathway in Endothelial Cells

Advanced Glycation End Products Activate the miRNA/RhoA/ROCK2 Pathway in Endothelial Cells

TNAP and EHD1 Are Over-Expressed in Bovine Brain Capillary Endothelial Cells after the Re-Induction of Blood-Brain Barrier Properties

Construction of nanometer cisplatin core-ferritin (NCC-F) and proteomic analysis of gastric cancer cell apoptosis induced with cisplatin released from the NCC-F

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