A digital image-based method employing a spot-test for quantification of ethanol in drinks

Benedetti, Luzia Pires dos Santos; Santos, Vagner Bezerra dos; Silva, Tiago; Filho, Edemar Benedetti; Martins, Valdomiro Lacerda; Fatibello‐Filho, Orlando

doi:10.1039/c5ay00529a

Cited by 76 publications

(37 citation statements)

References 33 publications

(44 reference statements)

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“…This work is related with a previous work reported by our research group, which ethanol content in drink samples was determined 35 . The most important features of this analytical approach are its low-cost and the portability of the instrumentation developed for the luminosity control; its low waste generation; and the ability to perform a significant number of samples simultaneously by using only one photography.…”

Section: Introductionsupporting

confidence: 56%

“…For image treatment the free software ImageJ was used, as well as Excel and Origin. Further details can be found in previous work 35 .…”

Section: Apparatus and Instrumentationmentioning

confidence: 99%

“…A survey of the literature demonstrates that digital image analysis methods have been developed and applied for the determination of different analytes in varied matrix samples, e.g. sodium in physiological serum, calcium in water and lithium in anti-depressive drugs 29 , total acidity in red wines 31 , sodium and calcium in powdered milk 32 , and for estimating the age of bloodstains 33 , adulterations to cow's milk 34 , and the ethanol content in alcoholic drinks 35 . Flow analysis systems have also been proposed using this type of analytical approach 36 .…”

Section: Introductionmentioning

confidence: 99%

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A digital image analysis method for quantification of sulfite in beverages

et al. 2015

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Section: Introductionsupporting

confidence: 56%

“…For image treatment the free software ImageJ was used, as well as Excel and Origin. Further details can be found in previous work 35 .…”

Section: Apparatus and Instrumentationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A digital image analysis method for quantification of sulfite in beverages

et al. 2015

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“…Existem vários exemplos relevantes descritos na literatura, tais como para a determinação de nitrito em alimentos, amostras ambientais e amostras clínicas, 4 na determinação do diclofenaco sódico, dipirona sódica e gluconato de cálcio em drogas injetáveis, 5 análise de chás, 6 quantificação de etanol em bebidas, 7 e quantificação de sulfito em bebidas. 8 Além disso, o uso de imagens digitais pode ser uma alternativa para ensaios clínicos, como demonstrado recentemente para creatinina 9 e determinação de glucose, 10 alanina aminotransferase.…”

Section: (1)unclassified

Determinação de fosfato em refrigerantes utilizando um scanner de mesa e análise automatizada de dados: um exemplo didático para ensino de química

Colzani¹,

Rodrigues²,

Fogaça³

et al. 2017

Quim. Nova

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PHOSPHATE COLORIMETRIC ANALYSIS USING A DESKTOP SCANNER AND AUTOMATED DIGITAL IMAGE ANALYSIS:A DIDATICAL EXAMPLE TO TEACH COLORIMETRIC ANALYSES. We propose here the quantification of the phosphorus content in cola drinks by molybdenum blue method. Molybdenum blue method was carried out in the wells of a microplate, where method miniaturization represents reduction in reagents required, improved safety, reduced waste stream, and increased sample throughput. The phosphate concentration was determinate using scanned images of the microplate. Extraction of RGB values was carried out using ImageJ, the pugling Readplate was used allowing extraction of RGB values of all wells simultaneously. A Microsoft Excel spreadsheet is afforded in Google Drive, it organize data taken from ImageJ to afford calibrations curves and phosphate concentration in cola drinks. Calibration curves were prepared at 500-2500 µg L -1 of P with good linearity (R > 0.95). Phosphate concentrations in cola drinks determinate using digital images method are statistically equivalent to concentrations determined using a spectroscopic method at a 95% confidence interval.Keywords: RGB; digital image; ImageJ; phosphate. INTRODUÇÃONas últimas décadas, vários métodos colorimétricos foram adaptados para microplacas, em especial, na área clínica e em análises ambientais. As vantagens de adaptar os métodos tradicionais são a redução no uso de reagentes e consequente menor geração de resíduos químicos. No entanto, o uso de técnicas de microescala empregando microplacas, em sala de aula, é provavelmente limitada pelo custo elevado (ordem de US$ 10.000) dos leitores de microplacas. 1 Normalmente, as imagens digitais utilizam o sistema Red, Green e Blue (RGB) para a definição de cores. Nesse sistema, cada tom de cor é definido por três canais: R (vermelho), G (verde) e B (azul), que variam como índices inteiros entre 0 e 255, onde o 0 corresponde à cor preta e o 255 corresponde ao R ou G ou B puros. Desta forma, no sistema RGB, um tom de cor corresponde a um ponto em um espaço tridimensional formado pelos eixos R, G e B, sendo possível a geração de 16 milhões de cores diferentes (256 3 ). 2A absorbância é definida de acordo com a Equação 1. A potência de um feixe (P), transmitida através de uma célula com a solução do analito, é comparada com a potência que atravessa uma célula idên-tica contendo somente o solvente ou o branco dos reagentes (P 0 ). De acordo com a lei Beer-Lambert, a absorbância (A) é proporcional a concentração do analito. (1)Kohl et al.3 desenvolveram uma prática de ensino que mostrou que o princípio da absorbância pode ser facilmente demonstrada utilizando soluções coloridas e análises de imagens digitais. A prática de ensino consistia em fotografar um conjunto de cubetas contendo corante amarelo em diferentes concentrações e uma cubeta contendo apenas água destilada. Os valores de G foram obtidos para as cubetas que continham o corante amarelo e G 0 foi obtido da cubeta que continha apenas água destilada. Posteriormente, estes valores foram u...

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“…A novel, interesting, and inexpensive technology for the quantitative determination of milk preservative (formaldehyde) was explored. In recent years, digital image analysis (DIA) or digital image-based (DIB) methods have been developed and applied in analytical chemistry for quantitation of analyte of interest (Benedetti et al, 2015). The same principle of smartphone-based chemistry instrumentation; digitization of colorimetric measurements (Chang 2012) was extended here for assessing the concentration of formaldehyde in milk.…”

Section: Materials a N D M E T H O D Smentioning

confidence: 99%

Novel Method for The Determination of Preservative (Formaldehyde) in Bovine Milk Through Smart Phone-Based Colorimetric Technology

Gupta¹,

Aulakh²,

Tomar³

2019

IJVSBT

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The use of formaldehyde as a preservative is a very common practice to decrease the microbial load and to increase the shelf life of the milk. Its addition in food has immense adverse public health significance. The present study explored a simple, low-cost, rapid, reproducible, and field applicable Smartphone-based colorimetric technology, which was standardized and in-house validated for the quantitative determination of formaldehyde in milk samples. The method had simple steps of spot-test reaction and digital image analysis with the Red Green Blue approach. The linearity of the method was shown by analytical curves ranging from 0.25 ppm to 4 ppm that were characterized by R2 > 0.99. The limit of detection of 0.31 ppm demonstrated the sensitivity of the method to estimate formaldehyde residues in milk. Thus, we developed an innovative technology that uses an easily available device with potential of on-site quantification of formaldehyde in the supply chain. This technique is not only beneficial for end-users but also helps in achieving extension goals, which emphasize on the transfer of technology, i.e., moving out of the lab to the land.

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A digital image-based method employing a spot-test for quantification of ethanol in drinks

Abstract: The digital image analysis together with spot-test based on a colorimetric assay produces a fast, low waste, screening and in situ analysis.

Cited by 76 publications

References 33 publications

A digital image analysis method for quantification of sulfite in beverages

A digital image analysis method for quantification of sulfite in beverages

Determinação de fosfato em refrigerantes utilizando um scanner de mesa e análise automatizada de dados: um exemplo didático para ensino de química

Novel Method for The Determination of Preservative (Formaldehyde) in Bovine Milk Through Smart Phone-Based Colorimetric Technology

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