2023
DOI: 10.3390/ijms241713405
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A Digital PCR Method Based on Highly Specific Taq for Detecting Gene Editing and Mutations

Bo Li,
Junhao Liu,
Qilai Huang

Abstract: Digital PCR (dPCR) has great potential for assessing gene editing or gene mutation due to its ability to independently inspect each DNA template in parallel. However, current dPCR methods use a fluorescence-labeled probe to detect gene variation events, and their ability to distinguish variated sequences from the wild-type sequence is limited by the probe’s tolerance to mismatch. To address this, we have developed a novel dPCR method that uses a primer instead of a probe to sense gene variation. The enhanced T… Show more

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