A direct 3D suspension near-field electrospinning technique for the fabrication of polymer nanoarrays

Nagle, Alexander R; Fay, Cormac; Xie, Zhigang; Wallace, Gordon G.; Wang, Xungai; Higgins, Michael J.

doi:10.1088/1361-6528/ab011b

Cited by 8 publications

(17 citation statements)

References 40 publications

(53 reference statements)

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“…A number of research groups have been investigating suspended fiber arrays using solution jet writing, with suspended fibers between the pillars of 3D electrodes. [31,32] Solution-based jetting techniques have traditionally proved challenging for the control of fiber placement and rapid solidification through solvent evaporation. However, such arrays were fabricated with variable spacing between fibers and some degree of over-lapping of fibers.…”

Section: Neurite Outgrowth From Explanted Dorsal Root Gangliamentioning

confidence: 99%

Design of Suspended Melt Electrowritten Fiber Arrays for Schwann Cell Migration and Neurite Outgrowth

Hrynevich

Achenbach

Jüngst

et al. 2021

Macromolecular Bioscience

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In this study, well-defined, 3D arrays of air-suspended melt electrowritten fibers are made from medical grade poly(ɛ-caprolactone) (PCL). Low processing temperatures, lower voltages, lower ambient temperature, increased collector distance, and high collector speeds all aid to direct-write suspended fibers that can span gaps of several millimeters between support structures. Such processing parameters are quantitatively determined using a "wedge-design" melt electrowritten test frame to identify the conditions that increase the suspension probability of long-distance fibers. All the measured parameters impact the probability that a fiber is suspended over multimillimeter distances. The height of the suspended fibers can be controlled by a concurrently fabricated fiber wall and the 3D suspended PCL fiber arrays investigated with early post-natal mouse dorsal root ganglion explants. The resulting Schwann cell and neurite outgrowth extends substantial distances by 21 d, following the orientation of the suspended fibers and the supporting walls, often generating circular whorls of high density Schwann cells between the suspended fibers. This research provides a design perspective and the fundamental parametric basis for suspending individual melt electrowritten fibers into a form that facilitates cell culture.

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Section: Neurite Outgrowth From Explanted Dorsal Root Gangliamentioning

confidence: 99%

Design of Suspended Melt Electrowritten Fiber Arrays for Schwann Cell Migration and Neurite Outgrowth

Hrynevich

Achenbach

Jüngst

et al. 2021

Macromolecular Bioscience

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“…In this study, relative humidity and flow rate were fixed, while varying the most readily controllable processes in two treatment groups, T1 and T2 (see Tables 1 and 2 The minimum integer working distance which could be accurately applied was 1 mm, given the accuracy of calibration and the varying degree of angular divergence of both electrode and emitter from upright. The maximum working distance was defined by what had previously been found to be effective 44 , where the working distance is typically no greater than 10 mm for solution based NFES.…”

Section: Effects Of Process Parameters On Nanoarraysmentioning

confidence: 99%

“…In our previous work, we developed a suspension near-field electrospinning (SNFES) instrument, which enabled fabrication of suspended fibrous nanoarrays between the pillars of 3D electrodes 44 . In this study, we investigate the effects of varying process parameters, such as speed, working distance, tip voltage, iteration and pattern variation, on 3D fiber arrays produced by the SNFES.…”

Section: Introductionmentioning

confidence: 99%

Patterning and process parameter effects in 3D suspension near-field electrospinning of nanoarrays

Nagle¹,

Fay²,

Wallace³

et al. 2019

Nanotechnology

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The extracellular matrix (ECM) contains nanofibrous proteins and proteoglycans. Nanofabrication methods have received growing interest in recent years as a means of recapitulating these elements within the ECM. Near-field electrospinning (NFES) is a versatile fibre deposition method, capable of layerby-layer nano-fabrication. The maximum layer height is generally limited in layer-by-layer NFES as a consequence of electrostatic effects of the polymer at the surface, due to residual charge and polymer dielectric properties. This restricts the total volume achievable by layer-by-layer techniques. Surpassing this restriction presents a complex challenge, leading to research innovations aimed at increasing patterning precision, and achieving a translation from 2D to 3D additive nanofabrication. Here we investigated a means of achieving this translation through the use of 3D electrode substrates. This was addressed by in-house developed technology in which selective laser melt manufactured standing pillar electrodes were combined with a direct suspension near-field electrospinning (SNFES) technique, which implements an automated platform to manoeuvre the pillar electrodes around the emitter in order to suspend fibres in the free space between the electrode support structures. In this study SNFES was used in multiple operation modes, investigating the effects of varying process parameters, as well as pattern variations on the suspended nanoarrays. Image analysis of the nanoarrays allowed for the assessment of fibre directionality, isotropy, and diameter; identifying optimal settings to generate fibres for tissue engineering applications.

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“…9,10 In a similar vein, more complicated 3D printing platforms were designed to allow for the fabrication of functional bionic organs with anatomic geometries, such an ear or heart. 11, 12 Interestingly, these ad-hoc 3D printing platforms can be applied to different fabrication modalities, such as electrospinning 13 or melt electro-writing, 14 to fabricate structures with nanometric elements and/or structure-hydrogel composites. 15,16 The ability to introduce multiple materials provides a route to engineering the appropriate combination of mechanical and bioactive properties.…”

Section: Introductionmentioning

confidence: 99%

“…Other approaches allow the introduction of multiple materials. For example, coaxial printing was established to allow for the fabrication of constructs with more complicated architectures, befitting certain tissue engineering applications such as generation of vascularized tissues. , In a similar vein, more complicated 3D printing platforms were designed to allow for the fabrication of functional bionic organs with anatomic geometries, such an ear or heart. , Interestingly, these ad-hoc 3D printing platforms can be applied to different fabrication modalities, such as electrospinning or melt electro-writing, to fabricate structures with nanometric elements and/or structure-hydrogel composites. , …”

Section: Introductionmentioning

confidence: 99%

Fabrication of Aligned Biomimetic Gellan Gum-Chitosan Microstructures through 3D Printed Microfluidic Channels and Multiple In Situ Cross-Linking Mechanisms

Robinson

Talebian

Foroughi

et al. 2020

ACS Biomater. Sci. Eng.

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In this study we use a combination of ionic-and photocross-linking to develop a fabrication method for producing biocompatible microstructures using a methacrylated gellan gum (a polyanion) and chitosan (a polycation) in addition to lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) as the photoinitiator. This work involves the development of a low-cost, portable 3D bioprinter and a customized extrusion mechanism for controlled introduction of the materials through a 3D printed microfluidic nozzle, before being cross-linked in situ to form robust microstructure bundles. The formed microstructures yielded a diameter of less than 1 μm and a tensile strength range of ∼1 MPa. This study is the first to explore and achieve GGMA:CHT microstructure fabrication by means of controlled in-line compaction and photo-cross-linking through 3D printed microfluidic channels.

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A direct 3D suspension near-field electrospinning technique for the fabrication of polymer nanoarrays

Abstract: A direct 3D suspension near-field electrospinning technique for A direct 3D suspension near-field electrospinning technique for the fabrication of A direct 3D suspension near-field electrospinning technique for the fabrication of polymer nanoarrays polymer nanoarrays

Cited by 8 publications

References 40 publications

Design of Suspended Melt Electrowritten Fiber Arrays for Schwann Cell Migration and Neurite Outgrowth

Design of Suspended Melt Electrowritten Fiber Arrays for Schwann Cell Migration and Neurite Outgrowth

Patterning and process parameter effects in 3D suspension near-field electrospinning of nanoarrays

Fabrication of Aligned Biomimetic Gellan Gum-Chitosan Microstructures through 3D Printed Microfluidic Channels and Multiple In Situ Cross-Linking Mechanisms

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