A direct analysis of lamellar x-ray diffraction from hydrated oriented multilayers of fully functional sarcoplasmic reticulum

Herbette, Leo G.; Marquardt, Julien; Scarpa, Antonio; Blasie, J. Kent

doi:10.1016/s0006-3495(77)85547-1

Cited by 171 publications

(121 citation statements)

References 22 publications

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“…For diffraction patterns from unoriented samples, the structure factor |F(h)| is set equal to [h 2 I(h)] 1/2 (33,34), where I(h) is the integrated intensity of the hth order peak. From the obtained four lamellar peaks (first to fourth order) in the small-angle region, 16 possible electron density profiles were all calculated.…”

Section: Electron Density Distribution Of Bmp Membranesmentioning

confidence: 99%

Differential Membrane Packing of Stereoisomers of Bis(monoacylglycero)phosphate

et al. 2006

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Bis(monoacylglycero)phosphate (BMP) reveals an unusual sn-1,sn-1′ stereoconfiguration of glycerophosphate. We synthesized sn- (3-myristoyl-2-hydroxy)glycerol-1-phospho-sn-1′-(3′-myristoyl-2′-hydroxy)glycerol (1,1′-DMBMP) and characterized the thermotropic phase behavior and membrane structure, in comparison with those of the corresponding sn-3:sn-1′ stereoisomer (3,1′-DMBMP), by means of differential scanning calorimetry (DSC), small-and wide-angle X-ray scattering (SAXS and WAXS, respectively), pressure-area (π-A) isotherms, epifluorescence microscopy of monolayers, and molecular dynamics (MD) simulations. In DSC, these lipids exhibited weakly energetic broad peaks with an onset temperature of 9°C for 1,1′-DMBMP and 18°C for 3,1′-DMBMP. In addition, a highly cooperative, strongly energetic transition peak was observed at ∼40°C for 1,1′-DMBMP and ∼42°C for 3,1′-DMBMP. These results are supported by the observation that 1,1′-DMBMP exhibited a larger phase transition pressure (π c ) than 3,1′-DMBMP. Small-and wide-angle X-ray scattering measurements identified these small and large energetic transitions as a quasi-crystalline (L c1 )-quasi-crystalline with different tilt angle (L c2 ) phase transition and an L c2 -L R main phase transition, respectively. X-ray measurements also revealed that these DMBMPs undergo an unbinding at the main phase transition temperature. The MD simulations estimated stronger hydrogen bonding formation in the 3,1′-DMBMP membrane than in 1,1′-DMBMP, supporting the experimental data.Bis(monoacylglycero)phosphate, also known as lysobisphosphatidic acid (LBPA), was first described in pig and rabbit pulmonary alveolar macrophages by Body and Gray (1). BMPs comprise less than 1% of the total phospholipids in most mammalian cells (2, 3). However, the content is increased to 15% in late endosomes (4). In the specific internal membrane domains of this organelle, this lipid comprises more than 70% of the total phospholipids (5).Because of the high level of accumulation in late endosomes, BMP is assumed to be important for the structural and functional integrity of the organelle. Indeed, a series of experiments has demonstrated that agents that perturb BMP induce alterations in the sorting and trafficking of proteins and/or the receptor in late endosomes accompanied by structural changes of the late edonsome organelle (4, 6) along with abnormal accumulation of cholesterol (7). Moreover, BMP is found to be essential for the activator-stimulated hydrolysis of ganglioside G M1 (8) and G M2 (9) and for the hydrolysis of ceramide by acid ceramidase (10).BMP has unique structural characteristics with various fatty acid compositions depending on the source. Whereas all other mammalian phospholipids display a sn-3-glycerophosphate stereoconfiguration, BMP possesses an sn-1:sn-1′ structure and one acyl group attached to each of the glycerol moieties (11-13). An sn-3:sn-1′ isomer has also been reported for BMP in BHK cells (14). However, the sn-3:sn-1′ isomer in mammalian cells is postulated to be...

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Section: Electron Density Distribution Of Bmp Membranesmentioning

confidence: 99%

Differential Membrane Packing of Stereoisomers of Bis(monoacylglycero)phosphate

et al. 2006

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“…Nerve myelin membrane can be swollen by use of different concentrations of glycerol or sucrose solutions, and since the structure is centrosymmetric, the phase choice for any one reflection is reduced to 2 4 , as was the case in the lipid bilayers previously discussed. Using the same swelling method, 1D electron density profiles of bleached and unbleached rod photoreceptor membranes (e.g., Corless 1972) and functional sarcoplasmic reticulum membranes (Herbette et al 1977) were obtained.…”

Section: Applications To Biological or Biologically Relevant Systemsmentioning

confidence: 99%

X-ray diffraction studies of oriented lipid bilayers

Katsaras¹

1995

Biochem. Cell Biol.

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This brief review of the X-ray diffraction technique used to study oriented lipid bilayer systems is primarily intended to demonstrate to the nonspecialist in the lipid field the amount of detailed information that can be obtained simply by visually inspecting the diffraction pattern and making some measurements with a rule and protractor. The information that can be extracted from X-ray diffraction data is illustrated by selected examples of our most recent work.

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“…The intensity profile is defined by (1) and (2). In previous Xray work (Lesslauer & Blasie, 1972;Schwartz, Cain, Dratz & Blasie, 1975;Herbette, Marquardt, Scarpa & Blasie, 1977), an autocorrelation function has been derived from Jobs(X) either by taking the Fourier transform of Jobs(X) and using the function at its end points or by assuming knowledge of l~2(X ). From (1) and (2), Job~(X ) after effective division by (i~2(X) is given by…”

Section: First Situationmentioning

confidence: 99%

Deconvolution analysis in membrane diffraction using the modulus of the continuous Fourier transform

Worthington¹

1981

J Appl Cryst

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A method of deconvolution using the modulus of the continuous Fourier transform of the unit cell is described. This method differs from previous deconvolution methods in membrane diffraction in that calculations are carried out in reciprocal space. The modulus profile is obtained from the continuous intensity transform which is itself the Fourier transform of the autocorrelation function. Sampling theorem methods are used to reconstruct the continuous Fourier transform of the unit cell. The various phase choices are examined and compared. In membrane diffraction, the autocorrelation function is derived in two distinct situations: when the interference function is broad as in the case of a few unit cells and when the membrane systems contain wide regions of constant electron density. It is concluded that, in the first situation, the derived autocorrelation function contains missing information and is incorrect. On the other hand, in the second situation, the derived autocorrelation function is a good representation of the true autocorrelation function.

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A direct analysis of lamellar x-ray diffraction from hydrated oriented multilayers of fully functional sarcoplasmic reticulum

Cited by 171 publications

References 22 publications

Differential Membrane Packing of Stereoisomers of Bis(monoacylglycero)phosphate

Differential Membrane Packing of Stereoisomers of Bis(monoacylglycero)phosphate

X-ray diffraction studies of oriented lipid bilayers

Deconvolution analysis in membrane diffraction using the modulus of the continuous Fourier transform

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