2005
DOI: 10.1002/anie.200461212
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A Direct Glimpse of Cross‐Hybridization: Background‐Passified Microarrays That Allow Mass‐Spectrometric Detection of Captured Oligonucleotides

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Cited by 21 publications
(16 citation statements)
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“…[25,26] Here, we show that DNA duplex formation retains its selectivity for nanoparticles as large as 60 nm in diameter that interact with surfaces, even when the binding processes occur concurrently with those of 15 nm particles. A 15 nm particle, as used in our procedure, carries approximately 125 DNA strands, whereas a 60 nm particle carries ap-proximately 2000 DNA strands, meaning that more than an order of magnitude difference in aggregate binding strength can be expected.…”
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confidence: 91%
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“…[25,26] Here, we show that DNA duplex formation retains its selectivity for nanoparticles as large as 60 nm in diameter that interact with surfaces, even when the binding processes occur concurrently with those of 15 nm particles. A 15 nm particle, as used in our procedure, carries approximately 125 DNA strands, whereas a 60 nm particle carries ap-proximately 2000 DNA strands, meaning that more than an order of magnitude difference in aggregate binding strength can be expected.…”
mentioning
confidence: 91%
“…Surface-bound DNA strands 13, 14, and 15 were immobilized as disulfides, using the methodology employed in our earlier work on DNA-coated gold slides. [26] The disulfides, when dissociating on the gold surface, produce immobilized DNA strands and one equivalent of methyl-terminated filler (see Fig. 2b).…”
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confidence: 99%
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“…15,16 In fact, despite high surface densities and the use of matrix enhancement, spot sizes in the millimeter range are needed for limit of detections (LODs) in the high nanomolar regime. 16 To enhance the sensitivity of MS detection, a barcode approach may be applied in which direct target detection is replaced by detection of a target-specific barcode compound existing in multiple copies per target molecule, as recently demonstrated by Qiu et al 17 Here, the binding of target DNA to DNA-modified gold nanoparticles was detected at a LOD in the sub-nanomolar regime while simultaneously identified by laser desorption/ionization time-of-flight (LDI-TOF) MS analysis of nanoparticle-attached poly(ethylene)glycol chains of different molecular weights, acting as barcodes. However, the identification of different PEG-chains was made from a homogeneous mixture of encoded gold particles and not on an individual particle basis, in contrast to the case in conventional suspension-or random arrays.…”
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confidence: 99%