A direct method for the formation of peptide and carbohydrate dendrimers

Preparation, Characterization and Evaluation of Targeting Potential of Amphotericin B-Loaded Engineered PLGA Nanoparticles

“…This resulted in amine-terminated PLGA. The mannose was conjugated to amine-terminated PLGA using reported method with slight modifications (19). Briefly, amineterminated PLGA (0.1 mmol) was dissolved in 2 mL tetrahydrofuran, D-mannose (8 mM) in 0.1 M sodium acetate buffer (pH 4.0) was added, and the mixture was agitated at ambient temperature for 2 days to ensure completion of reaction.…”

Multivalent Peptide and Protein Dendrimers Using Native Chemical Ligation

“…[1][2][3][4][5][6] Dendrimers are welldefined, hyperbranched polymers with a high density of functional groups and are therefore attractive scaffolds for multivalent display of natural ligands. [7][8][9] Dendrimers have been successfully applied in the design of multivalent sugar ligands, [10][11][12] but much less work has been reported on multivalent peptide and protein dendrimers. Solid-phase peptide synthesis has been used to construct dendritic peptide wedges by condensation of successive generations of lysine residues followed by functionalization of the a and e amine groups with peptides.…”

“…Convergent synthesis of peptide dendrimers has also been reported by condensation of ketone-functionalized polyamidoamine (PAMAM) dendrimers with aminooxy-functionalized peptides, but this method is unsuitable for native proteins. [9] The few reports of protein ligation to dendrimers typically involve the coupling of a single protein (for example, an antibody) using nonspecific conjugation chemistry that results in conjugation at multiple sites on the protein. Recently, the preparation of multivalent hemoglobin dendrimers was reported that involved specific conjugation of a fourth generation PAMAM dendrimer to hemoglobin using a hemoglobin-specific cross-linking agent.…”

Therapy and prophylaxis of inhaled biological toxins

“…Furthermore, botulinum toxin A has been reported to have a greater binding affinity for a dendritic polymer with six residues of the oligosaccharide portion of brain GT1b ganglioside attached to it than for the whole ganglioside (Schengrund et al, 1997). Because dendritic polymers (dendrimers) (Vogtle, 2000) also can be derivatized with peptide sequences (Mitchell et al, 1999), it is possible that diphtheria toxin (Brooke and Cha, 2000) and staphylococcal enterotoxin B (Arad et al, 2000) could be prevented from acting at their cell surface receptor sites. In fact, once the cell surface receptors are known in detail, this approach could be applicable to the neutralization of many more toxins.…”