A Discussion of Biological Safety Cabinet Decontamination Methods: Formaldehyde, Chlorine Dioxide, and Vapor Phase Hydrogen Peroxide

Czarneski, Mark A.; Lorcheim, Kevin

doi:10.1177/153567601101600104

Cited by 15 publications

(13 citation statements)

References 12 publications

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“… Notes: This table compares the three fumigants most commonly used in the laboratory to decontaminate MSCs (also refer to the studies by Czarneski and Lorcheim (2011) and HSE (2005b) ). MSCs that are being used for clean cell culture work will only need to be fumigated following any episodes of microbial contamination attributed to the cabinet and before changing filters, servicing, moving or disposing of the cabinet, depending on local rules and risk assessment.…”

Section: Box 1 Definitions Of Terms Frequently Used In Tissue Culturementioning

confidence: 99%

Guidelines for the use of cell lines in biomedical research

et al. 2014

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Cell-line misidentification and contamination with microorganisms, such as mycoplasma, together with instability, both genetic and phenotypic, are among the problems that continue to affect cell culture. Many of these problems are avoidable with the necessary foresight, and these Guidelines have been prepared to provide those new to the field and others engaged in teaching and instruction with the information necessary to increase their awareness of the problems and to enable them to deal with them effectively. The Guidelines cover areas such as development, acquisition, authentication, cryopreservation, transfer of cell lines between laboratories, microbial contamination, characterisation, instability and misidentification. Advice is also given on complying with current legal and ethical requirements when deriving cell lines from human and animal tissues, the selection and maintenance of equipment and how to deal with problems that may arise.

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Section: Box 1 Definitions Of Terms Frequently Used In Tissue Culturementioning

confidence: 99%

Guidelines for the use of cell lines in biomedical research

et al. 2014

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“…Primary methods for BSC decontamination are formaldehyde gas, vapor phase hydrogen peroxide, and chlorine dioxide gas. 7 The decision was made to use the formaldehyde system as it was inexpensive with regard to equipment and consumables, and purchase is not restricted in Southeast Asia despite the fact that it is an Occupational Safety and Health Administration (OSHA)-classified carcinogen. This required that appropriate respiratory protection be purchased for all trainees (Table 1) and that they be fit-tested and given respiratory protection training.…”

Section: Methodsmentioning

confidence: 99%

A Biological Safety Cabinet Certification Program

2016

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Biological safety cabinets (BSCs) are the primary means of containment used in laboratories worldwide for the safe handling of infectious microorganisms. They provide protection to the laboratory worker and the surrounding environment from pathogens. To ensure the correct functioning of BSCs, they need to be properly maintained beyond the daily care routines of the laboratory. This involves annual maintenance and certification by a qualified technician in accordance to the NSF/American National Standards Institute 49-2014 Biosafety Cabinetry: Design, Construction, Performance, and Field Certification. Service programs can be direct from the manufacturer or through third-party service companies, but in many instances, technicians are not accredited by international bodies, and these services are expensive. This means that a large number of BSCs may not be operating in a safe manner. In this article, we discuss our approach to addressing the lack of trained and qualified personnel in Thailand who can install, maintain, and certify BSCs in a cost-effective and practical manner. We initiated a program to create both local and regional capacity for repair, maintenance, and certification of BSCs and share our experiences with the reader.

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“…54 VHP was also evaluated for decontamination of BSCs with mixed results. 52,55,56 Fey and colleagues 57 showed that spore inactivation could be achieved only if parameters such as dehumidification (relative humidity should be below 40% to prevent the vaporized hydrogen peroxide from condensing out but high enough to kill spores), conditioning (vapor injection), and decontamination time were perfectly set up. In constrast to formaldehyde or VPH, aHP does not require any adjustment of the relative humidity or of the temperature in the room or in the cabinet.…”

Section: Benefits Of Aerosolized Plasma-activated Hydrogen Peroxide Smentioning

confidence: 99%

Plasma-Activated Aerosolized Hydrogen Peroxide (aHP) in Surface Inactivation Procedures

Freyssenet

Karlen

2018

Appl Biosaf.

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Introduction: Hydrogen peroxide is a strong oxidant that possesses an antimicrobial activity. It has been successfully used in surface/room decontamination processes either under the form of hydrogen peroxide vapor (HPV) or of vaporized hydrogen peroxide (VHP). Aerosolized hydrogen peroxide (aHP) offers a third alternative. The technology relies on the dispersion of aerosols of a hydrogen peroxide solution often complemented with silver cations. aHP provides an inexpensive and safe approach to treat contaminated rooms but sometimes fails to achieve the 6-log10 reduction limit in the number of viable microorganisms. Methods: Here, we used a venturi-based aHP generator that generates 4 mm in size aerosols from a 12% plasma-activated hydrogen peroxide solution free of silver cations. Results & Discussion: We could successfully and constantly inactivate bacterial growth from biological indicators containing at least 10 6 spores of Geobacillus stearothermophilus placed on stainless steel discs wrapped in Tyvek pouches. We could also show that the biological indicators placed at various locations in a class II biosafety cabinet were equally inactivated, showing that hydrogen peroxide aerosols migrate through HEPA filters. Conclusions: Considering that our method for aerosol generation is simple, reproducible, and highly effective at inactivating spores, our approach is expected to serve as a relatively cost effective alternative method for disinfecting potentially contaminated rooms or surfaces.

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A Discussion of Biological Safety Cabinet Decontamination Methods: Formaldehyde, Chlorine Dioxide, and Vapor Phase Hydrogen Peroxide

Cited by 15 publications

References 12 publications

Guidelines for the use of cell lines in biomedical research

Guidelines for the use of cell lines in biomedical research

A Biological Safety Cabinet Certification Program

Plasma-Activated Aerosolized Hydrogen Peroxide (aHP) in Surface Inactivation Procedures

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