A disposable chitosan-modified carbon fiber electrode for dengue virus envelope protein detection

Cavalcanti, Igor T.; Silva, B. V. M.; Peres, Newton G.; Moura, Patrícia; Sotomayor, Marı́a Del Pilar Taboada; Guedes, Maria Izabel Florindo; Dutra, Rosa F.

doi:10.1016/j.talanta.2012.01.002

Cited by 43 publications

(19 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…26,29 Chitosan was bound to the carbon cloth via the reaction between -COOH groups on the electrode surface and -NH 2 groups on the chitosan (Scheme 1A). Scanning electron microscopy revealed that a thin layer of chitosan covered the entire electrode surface, with pore sizes suitable for microbial access ( Fig.…”

Section: 63mentioning

confidence: 99%

Improved cathode materials for microbial electrosynthesis

Zhang

Nie

Bain

et al. 2013

Energy Environ. Sci.

361

298

View full text Add to dashboard Cite

Microbial electrosynthesis is a promising strategy for the microbial conversion of carbon dioxide to transportation fuels and other organic commodities, but optimization of this process is required for commercialization. Cathodes which enhance electrode-microbe electron transfer might improve rates of product formation. To evaluate this possibility, biofilms of Sporomusa ovata, which are effective in acetate electrosynthesis, were grown on a range of cathode materials and acetate production was monitored over time. Modifications of carbon cloth that resulted in a positive-charge enhanced microbial electrosynthesis. Functionalization with chitosan or cyanuric chloride increased acetate production rates 6-7 fold and modification with 3-aminopropyltriethoxysilane gave rates 3-fold higher than untreated controls. A 3-fold increase in electrosynthesis over untreated carbon cloth cathodes was also achieved with polyaniline cathodes. However, not all strategies to provide positively charged surfaces were successful, as treatment of carbon cloth with melamine or ammonia gas did not stimulate acetate electrosynthesis. Treating carbon cloth with metal, in particular gold, palladium, or nickel nanoparticles, also promoted electrosynthesis, yielding electrosynthesis rates that were 6-, 4.7-or 4.5-fold faster than the untreated control, respectively. Cathodes comprised of cotton or polyester fabric treated with carbon nanotubes yielded cathodes that supported acetate electrosynthesis rates that were $3-fold higher than carbon cloth controls. Recovery of electrons consumed in acetate was $80% for all materials. The results demonstrate that one approach to increase rates of carbon dioxide reduction in microbial electrosynthesis is to modify cathode surfaces to improve microbe-electrode interactions. Broader contextMicrobial electrosynthesis is a recently conceived bioenergy strategy in which microorganisms use electrons derived from electrodes to reduce carbon dioxide to organic products that are excreted from the cells. Any form of electrical energy can power microbial electrosynthesis, but when electricity is obtained from solar technologies and water is the source of electrons, microbial electrosynthesis is an articial form of photosynthesis with many potential advantages over biomassbased energy strategies. This study demonstrates that there are several strategies for modifying cathode surface properties that can enhance rates of microbial electrosynthesis.

show abstract

Section: 63mentioning

confidence: 99%

Improved cathode materials for microbial electrosynthesis

Zhang

Nie

Bain

et al. 2013

Energy Environ. Sci.

361

298

View full text Add to dashboard Cite

show abstract

“…These methods offer several advantages including the ability to differentiate dengue serotypes that have higher specificity when combined with real-time technology such as TaqMan assays [23], and are cheaper than virus isolation methods [22]. However, these methods require specialist operators, expensive reagents and apparatuses, thus restricting their ___________________________________________________________________________ 139 use in many developing countries [24][25]. Furthermore, they are also easily contaminated by non-template PCRs present in the laboratory environment [26].…”

Section: Genome Detectionmentioning

confidence: 99%

Recent Trends in Dengue Detection Methods Using Biosensors

Zainuddin

Nordin

Rahim

2018

IIUMEJ

View full text Add to dashboard Cite

Dengue illness is an infectious tropical disease, transmitted by Aedes mosquitos, that poses a serious health threat to the tropical world. This disease causes widespread infection worldwide, with about 50 million cases of dengue occurring per annum out of which 500,000 recorded cases of dengue hemorrhagic fever and 22,000 deaths. Currently, there are no effective vaccines available to prevent the spread of the infection. Accurate and rapid laboratory diagnostic tests are required for early detection to reduce patient mortality rate. In this paper, common laboratory diagnosis methods for detecting dengue virus infection are discussed. Currently, virus isolation, RT-PCR and serology methods provide the most direct and accurate response for detection of dengue. However, these methods require tedious steps, expensive requirements and expert staffs. Recent research have proposed the usage of biosensors as an alternative new technology for detection of dengue. In this work, various types of biosensors such as electrochemical, piezoelectric, and optical biosensor have been described and compared to evaluate their effectiveness in dengue detection. It is observed that the optical biosensor offers the best detection due to its high sensitivity as compared to others, although it is popularly known as an expensive method. Alternatively, the use of electrochemical and piezoelectric biosensors (QCM) is highly recommended for detection of dengue due to their ease-of-use, low cost, low reagent consumption, disposability, and minimal sample preparation. These approaches have the potential to improve the rate of survival, particularly in resource-limited countries. ABSTRAK: Virus denggi adalah penyakit berjangkit tropika bawaan nyamuk Aedes yang menimbulkan ancaman serius kepada kesihatan global. Penyakit ini menyebabkan jangkitan yang meluas di seluruh dunia, dengan kira-kira 50 juta kes denggi yang berlaku setiap tahun di mana 500,000 kes demam denggi berdarah dan 22,000 kematian direkodkan. Buat masa ini, tiada vaksin yang berkesan untuk mencegah penyebaran jangkitan ini. Ujian diagnostik makmal yang cepat dan tepat diperlukan untuk pengesanan denggi awal untuk mengurangkan kadar kematian pesakit. Dalam artikel ini, kaedah diagnosis makmal yang biasa dilakukan untuk mengesahkan jangkitan virus denggi akan dibincangkan. Pada masa kini, kaedah pengasingan virus, RT-PCR dan serologi adalah tindak balas yang paling cepat dan tepat untuk mengesan denggi. Walau bagaimanapun, kaedah-kaedah ini memerlukan langkah-langkah yang melecehkan, kos penyelenggaraan yang tinggi dan kakitangan yang terlatih. Penyelidikan terkini telah mencadangkan penggunaan biosensor sebagai teknologi baru alternatif untuk mengesan denggi. Dalam artikel ini juga pelbagai jenis biosensor seperti biosensor elektrokimia, piezoelektrik, dan biosensor optik telah dijelaskan dan dibandingkan untuk menilai keberkesanannya dalam pengesanan denggi. Difahamkan bahawa biosensor optik menawarkan pengesanan terbaik kerana kepekaannya yang tinggi berbanding dengan yang lain, walaupun ia dikenali sebagai kaedah berkos tinggi. Sebaliknya, biosensor elektrokimia dan piezoelektrik (QCM) sangat disyorkan untuk mengesan denggi kerana ia mudah digunakan, berkos rendah, penggunaan bahan uji yang terhad, boleh dipakai buang, dan mempunyai penyediaan sampel yang minima. Pendekatan-pendekatan ini berpotensi untuk meningkatkan kadar kemandirian di kawasan-kawasan sumber terhad.

show abstract

“…The best approach is to immobilize antibodies by their Fc regions. [12] This configuration has been achieved using different strategies, including by the use of protein A [13] or protein G, [14] via covalent immobilization through the oxidized sugar chains of the antibody, [15] and others. Besides orientation, it's important to consider antibody density on the electrode surface.…”

Section: Important Aspects For Immunosensor Developmentmentioning

confidence: 99%

Nanomaterials for Advancing the Health Immunosensor

Rodríguez¹,

Trindade²,

Cabral³

et al. 2015

Biosensors - Micro and Nanoscale Applications

View full text Add to dashboard Cite

Nanotechnoloμy has exerted a siμniλicant impact in the development oλ biosensors allowinμ more sensible analytical methods. In health applications, the main challenμe oλ the immunoassay is to reach the suitable limit oλ detection, recoμnizinμ diλλerent analytes in complex samples like whole blood, serum, urine, and other bioloμical λluids. Diλλerent nanomaterials, includinμ metallic, silica and maμnetic nanoparticles, quantum dots, carbon nanotubes, and μraphene, have been applied, mainly to improve charμe electron transλer, catalytic activity, amount oλ immobilized biomolecules, low-backμround current, siμnal-to-noise ratio that consequently increase the sensitivity oλ immunosensors. Given the μreat impact oλ nanotechnoloμy, this chapter intends to discuss new aspects oλ nanomaterials relatinμ to immunosensor advancement. Keywords:Immunosensors, immunoassay, nanosensor, nanomaterial . IntroductionA major challenμe λaced by health proμrams is the maintenance and availability oλ diaμnostic tests that are required not only in inpatient or outpatient hospital but also λor an improved epidemioloμical survey. In many cases, the absence oλ laboratory testinμ or delay oλ diaμnosis μenerates neμative economic impacts, resultinμ in unnecessary hospitalization, intercurrence, and in some cases implications on the μlobal liλe quality oλ patients and underreportinμ oλ surveillance. In this context, the development oλ practical, λast, and reliable analytical methods is imperative.Biosensors have been considered one oλ the more attractive analytical methods. They are biodevices capable oλ transλorminμ an interaction with speciλic analytes into an electrical siμnal by a transducer, includinμ a biorecoμnition element. [ ] Pharmaceutical industries and users oλ rapid tests λrom the United States and Europe are unanimous in statinμ that biosensors, mainly those based on point-oλ-care testinμ POCT , or bedside testinμs are a practical technoloμy, reμarded as a short-, medium-, and lonμ-term trend. Amonμ several advantaμes, POCT can provide immediate responses results in λew minutes or in real time , samples do not need to be transported to the analytical phase in situ monitorinμ and require μenerally small volumes oλ samples, and the users can be skilled or unskilled and present better costeλλective analyses compared with conventional technoloμies used in clinical diaμnostic userλriendly technoloμy . One oλ the most widely useλul POCT is the μlucometer, which measures μlucose levels with accuracy by requirinμ a sinμle drop oλ blood. The rapid μlucose measurement is very important in trials to avoid serious adverse eλλects stemminμ λrom diabetes, includinμ seizures, coma, or even death. Worldwide, some diabetic outpatients have been beneλited by POCTs.Althouμh there is a μreat promisinμ market dedicated to health λor the detection oλ diseases and therapeutic monitorinμ, biosensors are not yet entirely broadcast, especially those devoted to nonenzymatic reactions, i.e., biosensors based on the aλλinity between antiμen antibo...

show abstract

A disposable chitosan-modified carbon fiber electrode for dengue virus envelope protein detection

Cited by 43 publications

References 34 publications

Improved cathode materials for microbial electrosynthesis

Improved cathode materials for microbial electrosynthesis

Recent Trends in Dengue Detection Methods Using Biosensors

Nanomaterials for Advancing the Health Immunosensor

Contact Info

Product

Resources

About