A Disposable, Cyclo-Olefin Copolymer, RNA Microfluidic Sensor for Bacteria Detection

Prada, Jorge; Lang, Walter; Cordes, Christina; Harms, Carsten

doi:10.1109/icsens.2018.8589606

Cited by 2 publications

(2 citation statements)

References 14 publications

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“…Considering these requirements, the cyclic olefin copolymer (COC) was proposed as a suitable material [37,38]. DNA oligonucleotide probes are easily immobilized on the COC surface through UV irradiation without surface modification [39], making it well suited for the fabrication of a chipbased biosensor [40][41][42][43].…”

Section: Introductionmentioning

confidence: 99%

Rolling Circle Amplification-based Copper Nanoparticle Synthesis on Cyclic Olefin Copolymer Substrate and Its Application in Aptasensor

Kim

Lim

et al. 2022

Biotechnol Bioproc E

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This study aimed to develop a label-free fluorescent aptasensor for the detection of diazinon (DZN) on a cyclic olefin copolymer (COC) substrate. The aptasensor design was based on rolling circle amplification (RCA) technology and the use of self-assembled copper nanoparticles (CuNPs). A dual-function (DF) probe, capable of binding to circular DNA and an aptamer, was designed and immobilized on a COC-bottom 96-well plate. An aptamer was used for selective recognition of DZN, and the specific site of the aptamer that strongly reacted with DZN was successfully identified using circular dichroism (CD) analysis. In presence of DZN, the aptamer and DZN formed a strong complex, thus providing an opportunity for hybridization of the DF probe and circular DNA, thereby initiating an RCA reaction. Repetitive poly thymine (T) sequence with a length of 30-mer, generated in the RCA reaction, served as a template for the synthesis of fluorescent copper nanoparticles, emitting an orange fluorescence signal (at approximately 620 nm) proportional to the amount of RCA product, within 10 min under UV irradiation. The CuNP fluorescence was imaged and quantified using an image analysis software. A linear correlation of the fluorescence signal was confirmed in the DZN concentration range of 0.1-3 ppm, with a detection limit of 0.15 ppm. Adoption of a label-free detection method, utilizing RCA and fluorescent CuNPs on COC substrates, reduced the need for complex equipment and requirements for DZN analysis, thereby representing a simple and rapid sensing method circumventing the limitations of current complex and labor-intensive methods.

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Section: Introductionmentioning

confidence: 99%

Rolling Circle Amplification-based Copper Nanoparticle Synthesis on Cyclic Olefin Copolymer Substrate and Its Application in Aptasensor

Kim

Lim

et al. 2022

Biotechnol Bioproc E

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“…Small-sized portable microdevices based on various materials, such as glass [8], paper [9], silicon [10], polydimethylsiloxane [11,12], and cyclic olefin copolymers (COCs) [13,14], have been investigated for simplified onsite detection. COCs are suitable substrates for microarray chips because of their high chemical resistance, low water absorption, and high optical transparency [15][16][17][18]. They are a good alternative to conventional materials, such as glass, because fabricating microsystems, such as the reaction chamber and microfluidic channel, is easy and economical.…”

Section: Introductionmentioning

confidence: 99%

Microarray detection method for pathogen genes by on-chip signal amplification using terminal deoxynucleotidyl transferase

Kim

Lim

et al. 2022

Micro and Nano Syst Lett

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A microarray detection method based on on-chip signal amplification using terminal deoxynucleotidyl transferase (TdT) was developed to visualize pathogenic genes. Cyclic olefin copolymer (COC) substrate for microarrays was treated with oxygen plasma to induce hydrophilic surface properties. The capture probe DNA was immobilized on the COC surface by UV irradiation. The 3ʹ end of the capture probe DNA immobilized on the COC surface was modified with a phosphate group to provide resistance against the TdT reaction. Therefore, the TdT reaction was triggered only when the capture probe DNA acquired the target gene, and biotin-11-deoxyuridine triphosphate (b-dUTP) was continuously added to the 3ʹ end of the target gene. Thereafter, streptavidin-conjugated gold nanoparticles (s-AuNPs) tagged the poly uridine tails by the biotin–streptavidin interaction. The visual signal was amplified by silver enhancement in the presence of the s-AuNPs. The usefulness of this detection method was confirmed by analyzing four pathogens and allowing their visual identification.

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A Disposable, Cyclo-Olefin Copolymer, RNA Microfluidic Sensor for Bacteria Detection

Cited by 2 publications

References 14 publications

Rolling Circle Amplification-based Copper Nanoparticle Synthesis on Cyclic Olefin Copolymer Substrate and Its Application in Aptasensor

Rolling Circle Amplification-based Copper Nanoparticle Synthesis on Cyclic Olefin Copolymer Substrate and Its Application in Aptasensor

Microarray detection method for pathogen genes by on-chip signal amplification using terminal deoxynucleotidyl transferase

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