A disulphide isomerase gene (PDI-V) from Haynaldia villosa contributes to powdery mildew resistance in common wheat

Abstract

In this study, we report the contribution of a PDI-like gene from wheat wild relative Haynaldia villosa in combating powdery mildew. PDI-V protein contains two conserved thioredoxin (TRX) active domains (a and a′) and an inactive domain (b). PDI-V interacted with E3 ligase CMPG1-V protein, which is a positive regulator of powdery mildew response. PDI-V was mono-ubiquitinated by CMPG1-V without degradation being detected. PDI-V was located on H. villosa chromosome 5V and encoded for a protein located in the endoplasmic reticulum. Bgt infection in leaves of H. villosa induced PDI-V expression. Virus induced gene silencing of PDIs in a T. durum-H. villosa amphiploid compromised the resistance. Single cell transient over-expression of PDI-V or a truncated version containing the active TXR domain a decreased the haustorial index in moderately susceptible wheat cultivar Yangmai 158. Stable transgenic lines over-expressing PDI-V in Yangmai 158 displayed improved powdery mildew resistance at both the seedling and adult stages. By contrast over-expression of point-mutated PDI-VC57A did not increase the level of resistance in Yangmai 158. The above results indicate a pivotal role of PDI-V in powdery mildew resistance and showed that conserved TRX domain a is critical for its function.

Genome-wide characterization and expression profiling of PDI family gene reveals function as abiotic and biotic stress tolerance in Chinese cabbage (Brassica rapa ssp. pekinensis)

“…PDI genes have been previously shown to be contributed against powdery mildew resistance in common wheat [ 36 ]. In this study, we have analyzed expression profiles of these genes in response to Fusarium oxysporum f.sp.…”

Cloning and functional assessment of the protein disulfide isomerase (PDI-V) gene and its promoter sequences from Haynaldia villosa

“…Further characterization of the promoter region against powdery mildew suggested that the PromoPDI-V was barely active in response to Bgt. Our previous study showed the upregulation of PDI-V expression upon Bgt infection and its role in combating powdery mildew (Faheem et al, 2016). Therefore, we may speculate on two possibilities for the redundancy of PDI-V promoters against pathogens; firstly, the function of 5′-UTR.…”

Transient Overexpression of HvSERK2 Improves Barley Resistance to Powdery Mildew

“…The PCR product and the pAN580 vector were digested by XbaI and SmaI, and the fragments were ligated to produce the fusion gene expression vector p35S::HvSERK2-GFP::Nos3. For transient expression in onion, the constructs were delivered to epidermal cells on the adaxial surfaces of tissue peeled from an onion by particle bombardment, as described by Faheem et al [ 31 ]. GFP signals were assessed by OLYMPUS SZX16 (OLYMPUS, Tokyo, Japan) imaging 16–20 h after bombardment.…”

“…The HvSERK2 promoter was cloned into plant expression vector pAN580 [ 31 ] by replacing the CaMV 35S promoter to produce a pAN580:HvSERK2P:GFP plasmid ( Supplementary Materials Table S3 ). Second leaves of one-week-old barley seedlings were firmly placed on the 1% agar plates and were supplemented with 85 µM of benzimidazoles for the delivery of the pAN580:HvSERK2P:GFP plasmid into host cells by gene gun.…”