A DNA-barcoding approach to identifying juvenile freshwater mussels (Bivalvia:Unionidae) recovered from naturally infested fishes

Boyer, Sarah L.; Howe, Alexander A.; Juergens, Nathan W.; Hove, Mark C.

doi:10.1899/10-004.1

Cited by 42 publications

(21 citation statements)

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“…We recommend careful re‐examination of these taxa before taxonomic revisions. Additionally, our results showed that A. ligamentina was nested within Lampsilis and sister to L. higginsii , which is congruent with previous studies (Chapman et al., ; Boyer et al., ). The validity of Actinonaias species in the USA and Canada has been questioned because of their disjunct distributions from the type species of the genus, Actinonaias sapotalensis (Lea, 1841) from the Río Papaloapan in Veracruz, Mexico (Williams et al., ).…”

Section: Discussionsupporting

confidence: 93%

A comprehensive approach uncovers hidden diversity in freshwater mussels (Bivalvia: Unionidae) with the description of a novel species

et al. 2019

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Major geological processes have shaped biogeographical patterns of riverine biota. The Edwards Plateau of central Texas, USA, exhibits unique aquatic communities and endemism, including several species of freshwater mussels. Lampsilis bracteata (Gould, 1855) is endemic to the Edwards Plateau region; however, its phylogenetic relationship with other species in the Gulf coastal rivers and Mississippi River basin is unknown. We evaluated phylogenetic relationships, shell morphologies and soft anatomy characters of L. bracteata and a closely related congener, Lampsilis hydiana (Lea, 1838) throughout their ranges. Our results showed the presence of an undescribed species: Lampsilis bergmanni sp.n. Lampsilis bracteata and L. bergmanni sp.n. share similar shell morphologies and soft anatomy characters; however, they are genetically distinct. Geological processes, such as faulting and sea-level changes during the Miocene to Pliocene, are likely to have facilitated diversification of Lampsilis species, resulting in isolation of L. bracteata on the Edwards Plateau and diversification between L. bergmanni sp.n. and L. hydiana. We conclude that L. bracteata range is restricted to the Colorado River basin, whereas L. bergmanni sp.n. occurs only in upstream reaches of the Guadalupe River basin. Conservation actions are warranted for both species due to their restricted distributions and potential anthropogenic threats.

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Section: Discussionsupporting

confidence: 93%

A comprehensive approach uncovers hidden diversity in freshwater mussels (Bivalvia: Unionidae) with the description of a novel species

et al. 2019

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“…The information on leeches has been recently updated (Klemm et al, 2013, and references therein) and molecular approaches are being used to identify the glochidia of unionids (Gustafson and Iwamoto, 2005;Kneeland and Rhymer, 2007;Boyer et al, 2011).…”

Section: Knowledge Of Parasite Groupsmentioning

confidence: 99%

Parasites of Freshwater Fishes In North America: Why So Neglected?

Scholz

Choudhury

2014

Journal of Parasitology

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Fish parasitology has a long tradition in North America and numerous parasitologists have contributed considerably to the current knowledge of the diversity and biology of protistan and metazoan parasites of freshwater fishes. The Journal of Parasitology has been essential in disseminating this knowledge and remains a significant contributor to our understanding of fish parasites in North America as well as more broadly at the international level. However, with a few exceptions, the importance of fish parasites has decreased during the last decades, which is reflected in the considerable decline of funding and corresponding decrease of attention paid to these parasites in Canada and the United States of America. After the 'golden age' in the second half of the 20th Century, fish parasitology in Canada and the United States went in a new direction, driven by technology and a shift in priorities. In contrast, fish parasitology in Mexico has undergone rapid development since the early 1990s, partly due to extensive international collaboration and governmental funding. A critical review of the current data on the parasites of freshwater fishes in North America has revealed considerable gaps in the knowledge of their species composition, host specificity, life cycles, evolution, phylogeography, and relationships with their fish hosts. As to the key question, "Why so neglected?" this is probably because: (1) fish parasites are not in the forefront due to their lesser economic importance; (2) there is little funding for this kind of research, especially if a practical application is not immediately apparent; and (3) of shifting interests and a shortage of key personalities to train a new generation (they switched to marine habitats or other fields). Some of the opportunities for future research are outlined, such as climate change and cryptic species diversity. A significant problem challenging future research seems to be the loss of trained and experienced fish parasitologists. This has 2 major ramifications: the loss of expertise in identifying organisms that other biologists (e.g., ecologists, molecular biologists, evolutionists) work with, and an incomplete comprehension of ecosystem structure and function in the face of climate change, emerging diseases, and loss of biodiversity.

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“…from birds, moths and bivalves [113]–[115]. This may be related to the use of mean interspecific distances for plotting frequency distributions.…”

Section: Discussionmentioning

confidence: 99%

Pitfalls of Establishing DNA Barcoding Systems in Protists: The Cryptophyceae as a Test Case

Hoef‐Emden

2012

PLoS ONE

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A DNA barcode is a preferrably short and highly variable region of DNA supposed to facilitate a rapid identification of species. In many protistan lineages, a lack of species-specific morphological characters hampers an identification of species by light or electron microscopy, and difficulties to perform mating experiments in laboratory cultures also do not allow for an identification of biological species. Thus, testing candidate barcode markers as well as establishment of accurately working species identification systems are more challenging than in multicellular organisms. In cryptic species complexes the performance of a potential barcode marker can not be monitored using morphological characters as a feedback, but an inappropriate choice of DNA region may result in artifactual species trees for several reasons. Therefore a priori knowledge of the systematics of a group is required. In addition to identification of known species, methods for an automatic delimitation of species with DNA barcodes have been proposed. The Cryptophyceae provide a mixture of systematically well characterized as well as badly characterized groups and are used in this study to test the suitability of some of the methods for protists. As species identification method the performance of blast in searches against badly to well-sampled reference databases has been tested with COI-5P and 5′-partial LSU rDNA (domains A to D of the nuclear LSU rRNA gene). In addition the performance of two different methods for automatic species delimitation, fixed thresholds of genetic divergence and the general mixed Yule-coalescent model (GMYC), have been examined. The study demonstrates some pitfalls of barcoding methods that have to be taken care of. Also a best-practice approach towards establishing a DNA barcode system in protists is proposed.

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A DNA-barcoding approach to identifying juvenile freshwater mussels (Bivalvia:Unionidae) recovered from naturally infested fishes

Cited by 42 publications

References 50 publications

A comprehensive approach uncovers hidden diversity in freshwater mussels (Bivalvia: Unionidae) with the description of a novel species

A comprehensive approach uncovers hidden diversity in freshwater mussels (Bivalvia: Unionidae) with the description of a novel species

Parasites of Freshwater Fishes In North America: Why So Neglected?

Pitfalls of Establishing DNA Barcoding Systems in Protists: The Cryptophyceae as a Test Case

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