A DNA-based parity generator/checker for error detection through data transmission with visual readout and an output-correction function

Abstract

The first DNA-based molecular parity generator/checker, used for error detection through data transmission with fluorescent and visual readouts, has been constructed. The erroneous transmission can be readily distinguished by the naked eye using the G-quadruplex DNAzyme as a signal reporter of the visual outputs.

A simple, label-free, electrochemical DNA parity generator/checker for error detection during data transmission based on “aptamer-nanoclaw”-modulated protein steric hindrance

“…The platform of the pC was still the MCH/S/Au electrode, to which the third input P was brought to meet the requirements. The input variations of the 3-bit even pC that are demonstrated in Table 1 can be separated into two parts 24,28,29. For the first part (P = 0 states, entries 1 to 4), the outputs of the pC (Δ R ct was defined as output C now) were just the same as those of the above-constructed 2-bit even pG.…”

“…The logic circuit can be considered as an XOR gate, in which the DNA reactions were analogous to the 2-bit pG and have been illustrated above. For the second part (P = 1 states, entries 5 to 8), input P actually executes the XNOR function that is complementary to both inputs (D1 and D2) to change the number of 1's ( Σ a ) in the DnP string to even 24,28,29. The detailed DNA hybridizations of various entries (entries 5 to 8) are shown in Scheme 3.…”

“…All of the above describes the “error detection” procedure of the even pG/pC, and the odd pG/pC qualifies similar functions. Although scientists have made great efforts to advance the pG/pCs, most of the current pG/pCs are constructed upon semiconductor substrates,24–29 and only a few molecular pG/pCs have been achieved based on organic molecules or DNAs using optical (fluorescent or colorimetric) signals as outputs 28,29. To the best of our knowledge, no electrochemical DNA pG/pC system has ever been reported and further endeavours are required to realize it.…”

Enzyme‐Based Logic Gates and Networks with Output Signals Analyzed by Various Methods

“…Although the present review article is concentrated specifically on the read‐out methods for the output signals generated by the enzyme logic systems, other biomolecular computing assemblies, particularly including DNA‐based logic systems, can be combined with various detection methods discussed in the article. Particularly, catalytic reactions activated by DNAzymes in the presence of various combinations of inputs can be analyzed by the methods developed for the analysis of the biocatalytic logic systems based on the enzyme reactions .…”

Tyramine Hydrochloride Based Label‐Free System for Operating Various DNA Logic Gates and a DNA Caliper for Base Number Measurements

“…[11] Many DNA logic circuits have been reported to mimic basic, advanced, or even concatenatedl ogic computation. [11][12][13][14][15][16][17][18][19][20] Additionally,t he DNA system,w hich can not only operate logic computation, but also perform bioanalysis, is ap romising prospect for the combination of logic intelligence [21] with analysis [22,23] in the future. According to the output strategies, DNA logic gates can be divided into two kinds:l abeled and unlabeled ones.C ompared with the labeled strategy,w hich requires expensive chemical labels, unlabeledr eporter based DNA logic gates have been broadly constructed [15,16] as ar esult of their low cost and simple operation.…”