2006
DOI: 10.1093/nar/gkl702
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A DNA biochip for on-the-spot multiplexed pathogen identification

Abstract: Miniaturized integrated DNA analysis systems have largely been based on a multi-chamber design with microfluidic control to process the sample sequentially from one module to another. This microchip design in connection with optics involved hinders the deployment of this technology for point-of-care applications. In this work, we demonstrate the implementation of sample preparation, DNA amplification, and electrochemical detection in a single silicon and glass-based microchamber and its application for the mul… Show more

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Cited by 131 publications
(108 citation statements)
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“…Magnetic particles are increasingly used as tools for cell separation (6,12), DNA extraction (4,19), and antigen detection (7,17). Among the various magnetic particle composites developed to date, streptavidin-immobilized magnetic particles have shown great potential due to the stability of the biotin-streptavidin interaction and the resulting ability to separate target molecules specifically and efficiently (5,14,15,21,35). However, the synthesis of streptavidin-immobilized magnetic particles involves multiple steps, including synthesis of magnetic core particles, polymer coating, and immobilization of streptavidin, and each step increases the production time and cost.…”
mentioning
confidence: 99%
“…Magnetic particles are increasingly used as tools for cell separation (6,12), DNA extraction (4,19), and antigen detection (7,17). Among the various magnetic particle composites developed to date, streptavidin-immobilized magnetic particles have shown great potential due to the stability of the biotin-streptavidin interaction and the resulting ability to separate target molecules specifically and efficiently (5,14,15,21,35). However, the synthesis of streptavidin-immobilized magnetic particles involves multiple steps, including synthesis of magnetic core particles, polymer coating, and immobilization of streptavidin, and each step increases the production time and cost.…”
mentioning
confidence: 99%
“…34 Nucleic acid analysis has become an important tool for the identification of microorganisms such as pathogens, bacterium, and so on, which are commonly present in food and the environment. [35][36][37][38][39] The process is based on the principle of complementary base pairing, adenine-thymine, and cytosine-guanine in DNA. If the target nucleic acid sequence is known, complementary sequences can be synthesized, labeled, and then immobilized on the sensor.…”
Section: Nucleic Acid-based Bioreceptormentioning
confidence: 99%
“…For the past 20 years the most popular alternative to culture-based detection of pathogens has been molecular-based assays, such as quantitative polymerase chain reaction (qPCR), due to their speed, reliability, and sensitivity (Ishii et al 2013;Straub et al 2005;Yeung et al 2006). However, even molecular-based assays are unreliable when it comes to detection of microorganisms in environmental samples such as wastewater, soil, vegetable surfaces, or aerosols.…”
Section: Introductionmentioning
confidence: 99%