A DNA Origami Mechanical Device for the Regulation of Microcosmic Structural Rigidity

Wan, Neng; Hong, Zhouping; Wang, Huading; Fu, Xin; Zhang, Ziyue; Li, Chao; Xia, Han; Fang, Yan; Li, Maoteng; Zhan, Yi; Yang, Xiangliang

doi:10.1002/smll.201700866

Cited by 6 publications

(5 citation statements)

References 36 publications

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“…This can be attributed to the increasing rigidity of the nanostructure in the rotating conformation, reducing the tilt of the DNA structure between two membranes. 46 , 47 Additionally, the distance measured from MIET imaging is closely aligned with the cryo-TEM measurement, which give a mean distance of 27.6 ± 4.8 nm for the b1-2chol DNA nanobrush ( Figure S12 ). Second, we modified the nanobrush by shortening the side arms (b4) for the planar structures.…”

Section: Resultssupporting

confidence: 69%

“…Interestingly, even though the nanobrushes (b1; b2; b3) had the same arm lengths (64 bp +20 nt), the resulting intermembrane distance gradually increases from the planar to the twisted conformation (from 28.4 ± 1.9 nm to 32.1 ± 1.3 nm). This can be attributed to the increasing rigidity of the nanostructure in the rotating conformation, reducing the tilt of the DNA structure between two membranes. , Additionally, the distance measured from MIET imaging is closely aligned with the cryo-TEM measurement, which give a mean distance of 27.6 ± 4.8 nm for the b1-2chol DNA nanobrush (Figure S12). Second, we modified the nanobrush by shortening the side arms (b4) for the planar structures.…”

Section: Resultsmentioning

confidence: 97%

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Metal-Induced Energy Transfer (MIET) Imaging of Cell Surface Engineering with Multivalent DNA Nanobrushes

Wang,

Liu,

Han

et al. 2024

ACS Nano

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The spacing between cells has a significant impact on cell–cell interactions, which are critical to the fate and function of both individual cells and multicellular organisms. However, accurately measuring the distance between cell membranes and the variations between different membranes has proven to be a challenging task. In this study, we employ metal-induced energy transfer (MIET) imaging/spectroscopy to determine and track the intermembrane distance and variations with nanometer precision. We have developed a DNA-based molecular adhesive called the DNA nanobrush, which serves as a cellular adhesive for connecting the plasma membranes of different cells. By manipulating the number of base pairs within the DNA nanobrush, we can modify various aspects of membrane–membrane interactions such as adhesive directionality, distance, and forces. We demonstrate that such nanometer-level changes can be detected with MIET imaging/spectroscopy. Moreover, we successfully employed MIET to measure distance variations between a cellular plasma membrane and a model membrane. This experiment not only showcases the effectiveness of MIET as a powerful tool for accurately quantifying membrane–membrane interactions but also validates the potential of DNA nanobrushes as cellular adhesives. This innovative method holds significant implications for advancing the study of multicellular interactions.

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Section: Resultssupporting

confidence: 69%

Section: Resultsmentioning

confidence: 97%

Metal-Induced Energy Transfer (MIET) Imaging of Cell Surface Engineering with Multivalent DNA Nanobrushes

Wang,

Liu,

Han

et al. 2024

ACS Nano

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“…Note that HPDMs generate forces through the collective action of thousands of oligonucleotides. Therefore, our findings demonstrate that future generations of DNA walkers, − other hybridization-based DNA machines, ,− and non-DNA-based burnt bridge molecular motors can be used for engineering application that require pN-scale force generation. The diversity and potential of such engineering applications are illustrated by the ubiquity of biological motors like myosin and kinesin in eukaryotes and nanotechnology. , HPDMs are part of a growing trend towards the development of DNA-based devices that sense, transmit, and generate forces.…”

mentioning

confidence: 73%

Highly Polyvalent DNA Motors Generate 100+ pN of Force via Autochemophoresis

Blanchard

Bazrafshan

et al. 2019

Nano Lett.

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Motor proteins such as myosin, kinesin, and dynein are essential to eukaryotic life and power countless processes including muscle contraction, wound closure, cargo transport, and cell division. The design of synthetic nanomachines that can reproduce the functions of these motors is a longstanding goal in the field of nanotechnology. DNA walkers, which are programmed to “walk” along defined tracks via the burnt bridge Brownian ratchet mechanism, are among the most promising synthetic mimics of these motor proteins. While these DNA-based motors can perform useful tasks such as cargo transport, they have not been shown to be capable of cooperating to generate large collective forces for tasks akin to muscle contraction. In this work, we demonstrate that highly polyvalent DNA motors (HPDMs), which can be viewed as cooperative teams of thousands of DNA walkers attached to a microsphere, can generate and sustain substantial forces in the 100+ pN regime. Specifically, we show that HPDMs can generate forces that can unzip and shear DNA duplexes (∼12 and ∼50 pN, respectively) and rupture biotin–streptavidin bonds (∼100–150 pN). To help explain these results, we present a variant of the burnt-bridge Brownian ratchet mechanism that we term autochemophoresis, wherein many individual force generating units generate a self-propagating chemomechanical gradient that produces large collective forces. In addition, we demonstrate the potential of this work to impact future engineering applications by harnessing HPDM autochemophoresis to deposit “molecular ink” via mechanical bond rupture. This work expands the capabilities of synthetic DNA motors to mimic the force-generating functions of biological motors. Our work also builds upon previous observations of autochemophoresis in bacterial transport processes, indicating that autochemophoresis may be a fundamental mechanism of pN-scale force generation in living systems.

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“…Organization of receptor proteins on cell membrane is a strategic way to guide cell behaviors, but it is hindered by the lack of tool to determine the distance and space. [84,85] Nowadays, DNA origami-based PMSs with excellent programmability and addressability add up to a generous toolbox for cell regulations.…”

Section: Cell Regulationmentioning

confidence: 99%

DNA Origami‐Based Protein Manipulation Systems: From Function Regulation to Biological Application

Huang

Yin

et al. 2022

ChemBioChem

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Proteins directly participate in tremendous physiological processes and mediate a variety of cellular functions. However, precise manipulation of proteins with predefined relative position and stoichiometry for understanding protein‐protein interactions and guiding cellular behaviors is still challenging. With superior programmability of DNA molecules, DNA origami technology is able to construct arbitrary nanostructures that can accurately control the arrangement of proteins with various functionalities to solve these problems. Herein, starting from the classification of DNA origami nanostructures and the category of assembled proteins, we summarize the existing DNA origami‐based protein manipulation systems (PMSs), review the advances on the regulation of their functions, and discuss their applications in cellular behavior modulation and disease therapy. Moreover, the limitations and potential directions of DNA origami‐based PMSs are also presented, which may offer guidance for rational construction and ingenious application.

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A DNA Origami Mechanical Device for the Regulation of Microcosmic Structural Rigidity

Cited by 6 publications

References 36 publications

Metal-Induced Energy Transfer (MIET) Imaging of Cell Surface Engineering with Multivalent DNA Nanobrushes

Metal-Induced Energy Transfer (MIET) Imaging of Cell Surface Engineering with Multivalent DNA Nanobrushes

Highly Polyvalent DNA Motors Generate 100+ pN of Force via Autochemophoresis

DNA Origami‐Based Protein Manipulation Systems: From Function Regulation to Biological Application

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