A DNA vaccine against foot-and-mouth disease elicits an immune response in swine which is enhanced by co-administration with interleukin-2

Wong, Ho Tsun; Cheng, Samuel Chak-Sum; Sin, Fion Wan-Yee; Chan, Ella Wai-Ching; Sheng, Zu Tian; Xie, Youhua

doi:10.1016/s0264-410x(02)00212-8

Cited by 69 publications

(30 citation statements)

References 26 publications

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“…In the viral challenge, an 80% protection rate was obtained after three immunizations in swine. Unlike the previous 95% protection rate in swine immunized with synthetic vaccine and challenged with 10 4.5 TCID 50 FMDV (Wang et al 2002), and the 100% protection rate of DNA vaccine with 50 LD 50 (lethal dose in swine) virus challenge (Wong et al 2002), the challenge dose used in this study is 10 6.5 TCID 50 and higher than previous reports.…”

Section: Protection Of the Vp1àhctb Immunized Mice And Swine Against mentioning

confidence: 67%

A Novel Mucosal Vaccine Against Foot-and-Mouth Disease Virus Induces Protection in Mice and Swine

Song

Wang²,

Zheng³

et al. 2005

Biotechnol Lett

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Epitopes of a foot-and-mouth disease virus (FMDV) capsid protein VP1 complex and a chimera of 6xHis-tagged cholera toxin B subunit (hCTB) were expressed in Hansenula polymorpha and used together as a mucosal vaccine. Antibody and cytokine responses to VP1-hCTB vaccine and protection against FMDV were evaluated by ELISA and a virus challenge test in mice, respectively. VP1-hCTB directly enhanced the expression of interleukin-5 (IL-5) both in serum and supernatants of cultured spleen cells. After challenging suckling mice with 10(5) FMDV (=50% lethal dosage per mouse) a greater protection was seen after intraperitoneal and intranasal vaccinations than after oral vaccination. In swine immunized with VP1-hCTB, immune responses were achieved after three administrations, and the vaccine protected swine (80%) when challenged with 10(6.5) FMDV (=50% infectious dosage per swine). These results demonstrated the possibility of using CTB as a mucosal adjuvant to elicit protective immune responses against FMDV.

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Section: Protection Of the Vp1àhctb Immunized Mice And Swine Against mentioning

confidence: 67%

A Novel Mucosal Vaccine Against Foot-and-Mouth Disease Virus Induces Protection in Mice and Swine

Song

Wang²,

Zheng³

et al. 2005

Biotechnol Lett

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“…VP1 and IL-2 based chimeric DNA vaccine were developed which showed a better protection in swine (Wong et al, 2002). Recently, Gülçe et al (2013) reported that Coexpression of the Bcl-x Lanti apoptotic protein enhances the induction of Th1-like immune responses (in mice) immunized with DNA vaccines encoding FMDV B and T cell epitopes.…”

Section: New Generation Vaccine Trials Against Fmdmentioning

confidence: 99%

Foot-and-Mouth Disease, an Economically Important Disease of Animals

Chakraborty¹

2014

Adv. Anim. Vet. Sci.

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“…Protective immune response by immunization with a genetic vaccine was initially demonstrated in mice that underwent intramuscular injection of naked plasmid DNA encoding the internal nucleoprotein of the influenza virus [3]. Immunization using DNA vaccines is an approach that is being widely investigated to protect against a large number of infectious diseases including FMD [4][5][6][7][8][9]. Immunization with plasmid DNA elicits both cell-mediated and humoral immune responses [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, it has also been found that the B-cell and T helper (Th) cell responses are also elicited in DNA vaccination as antigen genes contained in the plasmids are translated and presented for surveillance. Wong et al [4] claim to have achieved complete protection in swine with DNA vaccine construct, consisting of two FMDV VP1 epitopes (amino acid residues 141-160 and 200-213) and demonstrated to have the ability to elicit both FMDV-specific T-cell proliferation and neutralization antibody against FMDV in vivo. Efficiency of DNA vaccines could be enhanced by co-administration of co-stimulatory molecules and suitable delivery systems [11].…”

Section: Introductionmentioning

confidence: 99%

Enhanced immune response of DNA vaccine (VP1-pCDNA) adsorbed on cationic PLG for foot and mouth disease in guinea pigs

et al. 2008

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Foot and mouth disease (FMD) is the major constraint to international trade in livestock and animal products. Though conventional vaccine has shown to provide protection, it has several limitations, like short duration of immunity and poor cell mediated immune response compared to DNA vaccines, which are known to induce both cell mediated as well as humoral responses. The present work envisages the production of DNA vaccine construct with partial 1D gene (coding for VP1) of FMDV type 'A' and studied the efficacy of the vaccine coated on cationic PLGA micro-particles in guinea pigs. Sequence coding for VP1 of serotype 'A' was amplified by PCR and cloned into mammalian expression vector, pCDNA-containing FMDV IRES. Expression of the construct was confirmed by transfection of the plasmid into BHK-21 cells followed by the protein profile by SDS-PAGE and Western blotting of the cell lysate. Guinea pigs were immunized with 25 mug of the vaccine construct intramuscularly, followed by a booster at 21st day. Sera from the animals of all the groups (pre-vaccinated, 14, 21, and 28 days of post-vaccination) was analyzed by ELISA and SNT. ELISA titers indicated significant improvement in the antibody titers in the PLG-coated DNA group (2.408 + 0.06), whereas the naked plasmid gave a titer of 1.505+. Serum neutralization titers were higher in PLG-coated vaccine group compared to the animals that received the naked DNA vaccine. Increased CTL response measured by MTT stimulation index (1.58 + 0.08) was observed in the case of PLG-coated DNA vaccine construct compared to the naked DNA vaccine (1.29 + 0.068). PLG-DNA vaccine construct conferred 100% protection to the animals when challenged with 100GpID50 of homologous virus compared to 50% protection in case of naked DNA vaccine construct. The present study has shown that adjuvantation with PLG markedly improved the efficacy of DNA vaccine against FMDV.

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A DNA vaccine against foot-and-mouth disease elicits an immune response in swine which is enhanced by co-administration with interleukin-2

Cited by 69 publications

References 26 publications

A Novel Mucosal Vaccine Against Foot-and-Mouth Disease Virus Induces Protection in Mice and Swine

A Novel Mucosal Vaccine Against Foot-and-Mouth Disease Virus Induces Protection in Mice and Swine

Foot-and-Mouth Disease, an Economically Important Disease of Animals

Enhanced immune response of DNA vaccine (VP1-pCDNA) adsorbed on cationic PLG for foot and mouth disease in guinea pigs

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