A double antibody sandwich enzyme-linked immunosorbent assay for detection of soft-shelled turtle iridovirus antigens

Zhang, M.; Yang, Jin-Yi; Lin, Xiaoyang; Zhu, Changlin; He, Jianan; Liu, H.; Lin, Tsai-Lu

doi:10.1016/j.jviromet.2010.04.004

Cited by 11 publications

(4 citation statements)

References 22 publications

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“…Another antigen assay has used anti-ranaviral monoclonal antibodies in a double antibody sandwich ELISA to detect viral particles in soft-shelled turtles (Zhang et al, 2010). In this case, the virion was detected with 98% specificity when compared with conventional PCR as the gold standard.…”

Section: Diagnostics and Surveillancementioning

confidence: 99%

Ranaviruses and reptiles

Wirth

Schwarzkopf

Skerratt

et al. 2018

PeerJ

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Ranaviruses can infect many vertebrate classes including fish, amphibians and reptiles, but for the most part, research has been focused on non-reptilian hosts, amphibians in particular. More recently, reports of ranaviral infections of reptiles are increasing with over 12 families of reptiles currently susceptible to ranaviral infection. Reptiles are infected by ranaviruses that are genetically similar to, or the same as, the viruses that infect amphibians and fish; however, physiological and ecological differences result in differences in study designs. Although ranaviral disease in reptiles is often influenced by host species, viral strain and environmental differences, general trends in pathogenesis are emerging. More experimental studies using a variety of reptile species, life stages and routes of transmission are required to unravel the complexity of wild ranavirus transmission. Further, our understanding of the reptilian immune response to ranaviral infection is still lacking, although the considerable amount of work conducted in amphibians will serve as a useful guide for future studies in reptiles.

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Section: Diagnostics and Surveillancementioning

confidence: 99%

Ranaviruses and reptiles

Wirth

Schwarzkopf

Skerratt

et al. 2018

PeerJ

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“…To develop a highly sensitive and specific DAS-ELISA, assay conditions were optimized according to the checkerboard titration method 34 , including the concentration of coating anti- S. eriocheiris rabbit serum and HRP-6H7. The optimized working concentration of coating pAb and HRP-6H7 were determined via the ratio of the OD value of the positive to the value of the negative (P/N).…”

Section: Methodsmentioning

confidence: 99%

Production and application of polyclonal and monoclonal antibodies against Spiroplasma eriocheiris

Zhang

Bao

Miao

et al. 2015

Sci Rep

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A new species of spiroplasma, Spiroplasma eriocheiris (S. eriocheiris), was identified as a lethal pathogen of tremor disease (TD) in Chinese mitten crab recently. In order to acquire appropriate biological and diagnostic tools for characterizing this newly discovered pathogen, 5 monoclonal antibodies (mAbs) and a polyclonal antibody (pAb) against S. eriocheiris were produced. Among the mAbs, 6F5, 7C8 and 12H5 lead to the deformation of S. eriocheiris. A peptide sequence, YMRDMQSGLPRY was identified as a mimic motif of MreB that is the cell shape determining protein of S. eriocheiris interacting with 3 mAbs. Furthermore, a double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for detection of S. eriocheiris was established using the mAb and pAb we prepared. It detected as low as 0.1 μg/mL of S. eriocheiris. No cross-reaction was observed with three other common bacteria (Pseudomonas aeruginosa, Escherichia coli, and Bacillus subtilis) and the hemolymph samples of healthy Eriocheir sinensis. Collectively, our results indicated that the mAbs and pAb we prepared could be used in the analysis of S. eriocheiris membrane proteins mimotope and development of a diagnostic kit for S. eriocheiris infections.

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“…Immunoassays used for the detection of iridoviruses include the enzyme-linked immunosorbent assay (ELISA) for the detection of EHNV [ 124 ], [ 16 ], SGIV [ 125 ], LMBV [ 126 ], RSIV [ 127 ] and other iridoviruses [ 128 , 129 ]. Currently, ELISA is becoming more quantitative and accurate, reaching detection limits as low as 10 3 PFU/mL due to ongoing technological refinements [ 130 , 131 ]. Immunohistochemistry (IHC) staining has been developed for the detection of viral antigens in infected tissues for viruses such as LMBV [ 132 ], RISV [ 133 ], TGIV [ 134 ].…”

Section: Diagnostic Assaysmentioning

confidence: 99%

Megalocytivirus and Other Members of the Family Iridoviridae in Finfish: A Review of the Etiology, Epidemiology, Diagnosis, Prevention and Control

Qin

Munang’andu

et al. 2023

Viruses

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Aquaculture has expanded to become the fastest growing food-producing sector in the world. However, its expansion has come under threat due to an increase in diseases caused by pathogens such as iridoviruses commonly found in aquatic environments used for fish farming. Of the seven members belonging to the family Iridoviridae, the three genera causing diseases in fish comprise ranaviruses, lymphocystiviruses and megalocytiviruses. These three genera are serious impediments to the expansion of global aquaculture because of their tropism for a wide range of farmed-fish species in which they cause high mortality. As economic losses caused by these iridoviruses in aquaculture continue to rise, the urgent need for effective control strategies increases. As a consequence, these viruses have attracted a lot of research interest in recent years. The functional role of some of the genes that form the structure of iridoviruses has not been elucidated. There is a lack of information on the predisposing factors leading to iridovirus infections in fish, an absence of information on the risk factors leading to disease outbreaks, and a lack of data on the chemical and physical properties of iridoviruses needed for the implementation of biosecurity control measures. Thus, the synopsis put forth herein provides an update of knowledge gathered from studies carried out so far aimed at addressing the aforesaid informational gaps. In summary, this review provides an update on the etiology of different iridoviruses infecting finfish and epidemiological factors leading to the occurrence of disease outbreaks. In addition, the review provides an update on the cell lines developed for virus isolation and culture, the diagnostic tools used for virus detection and characterization, the current advances in vaccine development and the use of biosecurity in the control of iridoviruses in aquaculture. Overall, we envision that the information put forth in this review will contribute to developing effective control strategies against iridovirus infections in aquaculture.

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A double antibody sandwich enzyme-linked immunosorbent assay for detection of soft-shelled turtle iridovirus antigens

Cited by 11 publications

References 22 publications

Ranaviruses and reptiles

Ranaviruses and reptiles

Production and application of polyclonal and monoclonal antibodies against Spiroplasma eriocheiris

Megalocytivirus and Other Members of the Family Iridoviridae in Finfish: A Review of the Etiology, Epidemiology, Diagnosis, Prevention and Control

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