A double-modification strategy for enhancing charge density of mono-sized beads for facilitated refolding of like-charged protein

Yang, Chunyan; Li, Ming; Dong, Xiaoyan; Sun, Yan

doi:10.1016/j.chroma.2013.05.059

Cited by 10 publications

(16 citation statements)

References 34 publications

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“…However, because of the limited specific surface area of the nonporous microspheres, the charge density achieved was low even with a doublemodification procedure. 5 Inspired by these findings, we propose that nanomaterials with an extremely high specific surface area and charge density would be more favorable in assisting the refolding of like-charged proteins.…”

Section: Introductionmentioning

confidence: 86%

Enhanced Protein Adsorption and Facilitated Refolding of Like-Charged Protein with Highly Charged Silica Nanoparticles Fabricated by Sequential Double Modifications

2015

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Silica nanoparticles (SNPs) were sequentially modified with poly(ethylenimine) (PEI) and 2-diethylaminoethyl chloride (DEAE) to prepare a series of positively charged SNPs-PEI and SNPs-PEI-DEAE. The sequential double-modification strategy produced a charge density as high as 1740 μmol/g (4524 μmol/mL), which offered a very high adsorption capacity for bovine serum albumin (314 mg/g). Most importantly, the highly charged SNPs-PEI and SNPs-PEI-DEAE could efficiently facilitate the refolding of like-charged protein at extremely low utilization. For instance, in the refolding of 1 mg/mL lysozyme, the refolding yield reached 75% with only 3.3 μL/mL SNPs-PEI-DEAE. The bead consumption was reduced by nearly 96% as compared to that of the charged microspheres used previously to reach a similar yield. The results proved that the polyelectrolyte-modified SNPs were promising for applications in facilitating like-charged protein refolding, and the research opened up a new way for biotechnology applications of highly charged nanoparticles.

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Section: Introductionmentioning

confidence: 86%

Enhanced Protein Adsorption and Facilitated Refolding of Like-Charged Protein with Highly Charged Silica Nanoparticles Fabricated by Sequential Double Modifications

2015

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“…PEI–CS–MSs were prepared according to the method of Yang et al with modifications. Typically, 1 g CS–MSs were added into 3 mL glutaraldehyde solution (5 %, L/L), followed by shaking at 30 °C and 100 r/min for 3 h. The microspheres were then collected and washed fully with deionized water.…”

Section: Methodsmentioning

confidence: 99%

Enhanced adsorption of xylenol orange from aqueous solutions by polyethylenimine‐grafted chitosan microspheres

Jing

Gao

2018

Can J Chem Eng

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To enhance the adsorption performance, chitosan microspheres (CS-MSs) were grafted with polyethylenimine (PEI), a cationic compound with abundant amine groups. The resulting PEI-grafted CS-MSs (PEI-CS-MSs) were used for the adsorption of xylenol orange (XO), an anionic dye. The results show that PEI-CS-MSs were in regular spherical shape without porous structure. XO molecules were adsorbed onto the amine groups of PEI-CS-MSs through electrostatic interaction. The maximum adsorption capacity of XO onto PEI-CS-MSs was 816.33 mg/g at 303 K, being much higher than that onto CS-MSs. The adsorption process could be properly described by the pseudo-second-order kinetic and Langmuir models. The adsorption of XO was a spontaneous process with exothermic nature. In addition, the adsorption capacity only decreased by 0.52 % after seven cycles of adsorption-desorption, indicating the excellent reusability of PEI-CS-MSs.

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“…The CS–PEI microspheres were prepared following the method reported previously with modifications . Typically, CS microspheres (0.34 g) were added into 20 mL of 10% (v/v) glutaraldehyde solution (50 mmol/L borax–boric acid buffer, pH 9.0), and the mixture was stirred at 30 °C and 100 rpm for 2 h. Afterwards, the microspheres were washed with deionized water to remove unreacted glutaraldehyde.…”

Section: Methodsmentioning

confidence: 99%

Chitosan microspheres modified with poly(ethylenimine) enhance the adsorption of methyl orange from aqueous solutions

Jing

Gao

Yang

2016

Asia-Pacific J Chem Eng

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Chitosan (CS) microspheres were prepared by emulsion crosslinking, and the surfaces of the spheres were modified with poly(ethylenimine) (PEI) for preparing efficient adsorbents. Consequently, a series of PEI-modified CS (CS-PEI) microspheres with different ion exchange capacities (IECs) was prepared and used for the adsorption of methyl orange (MO). The adsorption performance of CS-PEI increased significantly with the increase of the sphere IECs, and the maximum adsorption capacity of 400.00 mg/g was achieved at 303 K with CS-PEI-1275, which had the highest IEC in the experiments. The adsorption process of MO well fitted the pseudo-second-order kinetic and Langmuir isotherm models, and the process was favorable at low temperature. Thermodynamic analyses showed that the adsorption of MO by CS-PEI was a spontaneous and exothermic reaction. Furthermore, CS-PEI exhibited good reusability within seven cycles of adsorption-desorption.

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A double-modification strategy for enhancing charge density of mono-sized beads for facilitated refolding of like-charged protein

Cited by 10 publications

References 34 publications

Enhanced Protein Adsorption and Facilitated Refolding of Like-Charged Protein with Highly Charged Silica Nanoparticles Fabricated by Sequential Double Modifications

Enhanced Protein Adsorption and Facilitated Refolding of Like-Charged Protein with Highly Charged Silica Nanoparticles Fabricated by Sequential Double Modifications

Enhanced adsorption of xylenol orange from aqueous solutions by polyethylenimine‐grafted chitosan microspheres

Chitosan microspheres modified with poly(ethylenimine) enhance the adsorption of methyl orange from aqueous solutions

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