A Drimaren Blue X3LR dye decolorizing enzyme from Funalia trogii: one step isolation and identification

Evaluation of some white-rot fungi for their potential to decolourise industrial dyes

“…Recently, white-rot fungi and their enzymes have been reported to reduce the toxicity of anthraquinonic and triphenylmethane dyes [5,16]. The studies highlight the role of purified laccases in detoxification of malachite green and remazol brilliant blue R. Further the bacterial degradation of these dyes requires their intracellular uptake; whereas fungal systems, especially the white-rot, completely degrade by their extracellular enzymes which are capable of breaking a variety of heterogeneous molecular bonds [16,17]. The better effectivity of fungi for bioremediation of dye-polluted sites is attributed to the penetration ability of their hyphae [18] and their capability to degrade a wide variety of toxic compounds [19].…”

Decolorization of Azo Dyes by White Rot Fungi

“…The pigments found in other lignocellulosic substrates, such as wheat bran and wheat straw, could interfere in the dye decolorization experiments [13]. SSF containing wheat bran and soybean as a substrate was chosen for the production of ligninolytic enzymes for Funalia trogii ATCC200800 [18] as it mimics the natural environment of the WRF and permits the concentration of dyes by absorption process prior to biological treatment [4,38,39]. It is possible to stimulate the yield of laccase activity of Trametes versicolor by using several agricultural wastes [40].…”

“…The degradation of the disazo dye Chicago Sky Blue 6B by a purified laccase from Pycnoporus cinnabarinus showed a band having a molecular size of 63 kDa determined by SDS-PAGE [50]. Unyayar et al had reported the Drimarene Blue X3LR decolourizing enzymatic activity in the culture filtrate of F. trogii by using SDS-PAGE [18]. In this method, two SDS-PAGEs were performed.…”

“…This colourless zone turned into orange colour after incubation with guaicol, which is a classical indicator and substrate of laccase (Lane C, Fig. 3), and so it was concluded that this enzyme responsible for decolourization of Drimarene Blue X3LR was a laccase [18].…”

“…The use of lignin-degrading white rot fungi (WRF) has attracted increasing scientific attention, as these organisms are able to degrade a wide range of recalcitrant organic compounds. Their lignin modifying enzymes (LME), that is MnP, LiP and laccases, are directly involved in the degradation of not only lignin in their natural lignocellulosic substrates [9,10] but also various xenobiotic compounds [11,12] including dyes [13][14][15][16][17][18]. Peroxidases and laccases of WRF are oxidative enzymes, which do not need any other cellular components to work.…”