The transportation sector is a major contributor to greenhouse gas (GHG) emissions, accounting for 14% of global emissions in 2010 according to the United States Environmental Protection Agency. In Quebec, this share amounts to 43%, of which 80% is caused by road transport according to the MinistÉre de l’Environnement et de la Lutte contre les changements climatiques of QuÅbec. It is therefore essential to support the actions taken to reduce GHGs emissions from this sector and to quantify the impact of these actions. To do so, accurate and reliable emission models are needed. Driving cycles are defined as speed profiles over time and they are a key element of emission models. They represent driving behaviors specific to various road types in each region. The most widely used method to construct driving cycles is based on Markov chains and consists of concatenating small sections of speed profiles, called microtrips, following a transition matrix. Two of the main steps involved in the development of driving cycles are microtrip segmentation and microtrip classification. In this study, several combinations of segmentation and clustering methods are compared to generate the most reliable driving cycle. Results show that segmentation of microtrips with a fixed distance of 250 m and clustering of the microtrips by applying a principal component analysis on many key parameters related to their speed and acceleration provide the most accurate driving cycles.