A drug-free strategy to combat bacterial infections with magnetic nanoparticles biosynthesized in bacterial pathogens

Ghosh, Deepa; Kaushik, Swati; Thomas, Jijo; Panwar, Vineeta; Murugesan, Preethi; Chopra, Vianni; Salaria, Navita; Singh, Rohit; Roy, Himadri Shekhar; Kumar, Rajesh; Gautam, Vikas

doi:10.1039/d1nr07435k

Cited by 6 publications

(5 citation statements)

References 71 publications

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“…Hydrogen peroxide (200 µM) was used as a positive control. The ROS generated in the supernatant was determined at 488nm excitation and 530nm emission using Tecan plate reader (23).…”

Section: Determination Of Ros Generation In Bacterial Specimens By Vg...mentioning

confidence: 99%

VG111, A novel formulation (VG111) demonstrating clinical evidence of anti-pathogenic activity and accelerated wound healing in humans and companion animals

Singhal¹,

Kaushik

Panda

et al. 2023

Preprint

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VG111, a novel natural product formulation developed at PGIMER, Chandigarh is an attempt to meet the requirements of an ideal wound healing product. The present study demonstrates the efficacy of VG111 in antimicrobial assay, in tissue-regeneration,and in direct application-based wound healing. VG111 was able to kill major human pathogens, including methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli, carbapenem-resistant Acinetobacter baumannii (CRAB), while biofilm clearance was observed for Pseudomonas aeruginosa PA14 and Stenotrophomonas sepilia. Scratch assay and cytokine estimation in VG111-treated RAW464.7 cell lines show that it enhances cell migration and limits host inflammatory response by lowering IL-6 and TNF-α levels. VG111 application in canine and human patients’ representative cases provides evidence of remarkable wound healing and improved recovery. In particular, its potential to treat aggravated wounds in diabetic patients without the need of administering additional antibiotics in patients on steroids and obviating the need of skin graft adds on to make this natural product formulation to be a game-changer in the segment.

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“…Hydrogen peroxide (200 µM) was used as a positive control. The ROS generated in the supernatant was determined at 488nm excitation and 530nm emission using Tecan plate reader (23).…”

Section: Determination Of Ros Generation In Bacterial Specimens By Vg...mentioning

confidence: 99%

VG111, A novel formulation (VG111) demonstrating clinical evidence of anti-pathogenic activity and accelerated wound healing in humans and companion animals

Singhal¹,

Kaushik

Panda

et al. 2023

Preprint

Self Cite

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“…Magnetic hyperthermia was originally proposed in 1957 by Gilchrist et al It involves the use of the interaction of magnetic nanoparticles (MNPs) and alternating or radio magnetic fields to generate localized thermal energy for combating harmful cells. As a promising technique, the most famous and broad application of magnetic hyperthermia is noninvasive and targeted treatment for various types of cancers. − Nowadays, magnetic hyperthermia is also employed to kill pathogenic bacteria and treat infectious diseases. − Unlike direct killing of harmful cells by high heat, magnetic hyperthermia also stimulates drug release to achieve the goal of disease treatment, activities membrane receptor proteins or gene promoters, − and controls neural activity . Obviously, the principle behind these applications is the high temperature generated by magnetic hyperthermia.…”

Section: Introductionmentioning

confidence: 99%

Ca²⁺ Overload Decreased Cellular Viability in Magnetic Hyperthermia without a Macroscopic Temperature Rise

Chen,

Wang

et al. 2024

ACS Biomater. Sci. Eng.

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Magnetic hyperthermia is a crucial medical engineering technique for treating diseases, which usually uses alternating magnetic fields (AMF) to interplay with magnetic substances to generate heat. Recently, it has been found that in some cases, there is no detectable temperature increment after applying an AMF, which caused corresponding effects surprisingly. The mechanisms involved in this phenomenon are not yet fully understood. In this study, we aimed to explore the role of Ca 2+ overload in the magnetic hyperthermia effect without a perceptible temperature rise. A cellular system expressing the fusion proteins TRPV1 and ferritin was prepared. The application of an AMF (518 kHz, 16 kA/m) could induce the fusion protein to release a large amount of iron ions, which then participates in the production of massive reactive oxygen radicals (ROS). Both ROS and its induced lipid oxidation enticed the opening of ion channels, causing intracellular Ca 2+ overload, which further led to decreased cellular viability. Taken together, Ca 2+ overload triggered by elevated ROS and the induced oxidation of lipids contributes to the magnetic hyperthermia effect without a perceptible temperature rise. These findings would be beneficial for expanding the application of temperature-free magnetic hyperthermia, such as in cellular and neural regulation, design of new cancer treatment methods.

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“…MNPs are used in a wide variety of fields, especially in biomedical applications [ 5 ] ( Figure 1 ). MNPs have demonstrated utility as a medical imaging contrast agent [ 6 , 7 , 8 ], as a carrier for pharmaceutical agents [ 9 , 10 ], and in cancer therapy through magnetically induced hyperthermia (MHT) [ 11 , 12 , 13 ], photodynamic therapy (PDT) [ 6 , 14 , 15 ], and photothermal therapy (PTT) [ 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

“…MNPs are used in a wide variety of fields, especially in biomedical applications [5] (Figure 1). MNPs have demonstrated utility as a medical imaging contrast agent [6][7][8], as a carrier for pharmaceutical agents [9,10], and in cancer therapy through magnetically induced hyperthermia (MHT) [11][12][13], photodynamic therapy (PDT) [6,14,15], and photothermal therapy (PTT) [16][17][18]. On the other hand, MNPs have garnered significant interest as valuable tools for in vitro diagnosis with a variety of appealing applications such as trace analysis, magnetic separation, quantitative detection, and rapid testing, making them highly suitable for a range of biomedical uses [19].…”

Section: Introductionmentioning

confidence: 99%

The Recent Applications of Magnetic Nanoparticles in Biomedical Fields

Hong,

Wang,

Zheng

et al. 2024

Materials

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Magnetic nanoparticles (MNPs) have found extensive application in the biomedical domain due to their enhanced biocompatibility, minimal toxicity, and strong magnetic responsiveness. MNPs exhibit great potential as nanomaterials in various biomedical applications, including disease detection and cancer therapy. Typically, MNPs consist of a magnetic core surrounded by surface modification coatings, such as inorganic materials, organic molecules, and polymers, forming a nucleoshell structure that mitigates nanoparticle agglomeration and enhances targeting capabilities. Consequently, MNPs exhibit magnetic responsiveness in vivo for transportation and therapeutic effects, such as enhancing medical imaging resolution and localized heating at the site of injury. MNPs are utilized for specimen purification through targeted binding and magnetic separation in vitro, thereby optimizing efficiency and expediting the process. This review delves into the distinctive functional characteristics of MNPs as well as the diverse bioactive molecules employed in their surface coatings and their corresponding functionalities. Additionally, the advancement of MNPs in various applications is outlined. Additionally, we discuss the advancements of magnetic nanoparticles in medical imaging, disease treatment, and in vitro assays, and we anticipate the future development prospects and obstacles in this field. The objective is to furnish readers with a thorough comprehension of the recent practical utilization of MNPs in biomedical disciplines.

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A drug-free strategy to combat bacterial infections with magnetic nanoparticles biosynthesized in bacterial pathogens

Abstract: The extensive and indiscriminate use of antibiotics in the ongoing COVID-19 pandemic might significantly contribute to the growing number of multiple drug resistant (MDR) bacteria. With the dwindling pipeline of...

Cited by 6 publications

References 71 publications

VG111, A novel formulation (VG111) demonstrating clinical evidence of anti-pathogenic activity and accelerated wound healing in humans and companion animals

VG111, A novel formulation (VG111) demonstrating clinical evidence of anti-pathogenic activity and accelerated wound healing in humans and companion animals

Ca²⁺ Overload Decreased Cellular Viability in Magnetic Hyperthermia without a Macroscopic Temperature Rise

The Recent Applications of Magnetic Nanoparticles in Biomedical Fields

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A drug-free strategy to combat bacterial infections with magnetic nanoparticles biosynthesized in bacterial pathogens

Abstract: The extensive and indiscriminate use of antibiotics in the ongoing COVID-19 pandemic might significantly contribute to the growing number of multiple drug resistant (MDR) bacteria. With the dwindling pipeline of...

Cited by 6 publications

References 71 publications

VG111, A novel formulation (VG111) demonstrating clinical evidence of anti-pathogenic activity and accelerated wound healing in humans and companion animals

VG111, A novel formulation (VG111) demonstrating clinical evidence of anti-pathogenic activity and accelerated wound healing in humans and companion animals

Ca2+ Overload Decreased Cellular Viability in Magnetic Hyperthermia without a Macroscopic Temperature Rise

The Recent Applications of Magnetic Nanoparticles in Biomedical Fields

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Product

Resources

About

Ca²⁺ Overload Decreased Cellular Viability in Magnetic Hyperthermia without a Macroscopic Temperature Rise