A Dry Artificial Seed System for Brassica Crops

Takahata, Y.; Wakui, Kenji; Kaizuma, Norihiko; Brown, Daniel C.

doi:10.17660/actahortic.1992.319.49

Cited by 8 publications

(8 citation statements)

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“…The frequencies of germination and plant regeneration of desiccated embryos that were not stored were 90% and 89% for rapeseed and 100% and 58% for Chinese cabbage, respectively (Tables1and 2). These results are consistent with our previous ones [8,10].…”

Section: Resultssupporting

confidence: 94%

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Long-term Storage and Cryopreservation of Desiccated Microspore-derived Embryos in Brassica spp.

Wakui

Sato

Takahata

et al. 1998

Plant Biotechnology

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Section: Resultssupporting

confidence: 94%

“…microspore-derived embryos was induced by exogenous application of abscisic acid (ABA) [8][9][10]. Naked desiccated embryos of B, oleracea [9] and B. napes [8] maintained their viability for 3 and 6 months, respectively. However, longevity of the embryos and the influence of storage conditions on embryo viability have not been elucidated.…”

Section: Introductionmentioning

confidence: 99%

Long-term Storage and Cryopreservation of Desiccated Microspore-derived Embryos in Brassica spp.

Wakui

Sato

Takahata

et al. 1998

Plant Biotechnology

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“…Desiccation tolerance of microspore‐derived embryos of B. napus and B. campestris was induced by application of 100–10 µ M ABA for 7 days (Takahata et al., 1992, Wakui et al. 1994).…”

Section: Resultsmentioning

confidence: 99%

“…In order to understand the mechanism of desiccation tolerance of embryos, it is important to know the relationship between induction of desiccation tolerance and exposure time to ABA. The desiccation tolerance of the somatic embryos of several plants such as carrot, alfalfa, celery and Brassica was reported to be induced by more than 5 days exposure to ABA (Kitto and Janick 1985, Senaratna et al. 1990, Kim and Janick 1991, Takahata et al.…”

Section: Discussionmentioning

confidence: 99%

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Isolation and expression of Lea gene in desiccation‐tolerant microspore‐derived embryos in Brassica spp.

Wakui

Takahata

2002

Physiologia Plantarum

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Desiccation tolerance can be induced in microspore-derived embryos of Brassica spp. by application of abscisic acid (ABA). As part of our effort to elucidate the molecular mechanism in induction of desiccation tolerance of embryos, we have isolated Lea genes and examined their expression in microspore-derived embryos of B. napus and B. campestris. The cDNA clones of Lea genes were isolated from desiccation-tolerant microspore-derived embryos of both species. Two cDNA clones of B. napus and B. campestris shared a similar homology of 92.9% identity between the two deduced proteins. These proteins also had high homology with Lea 76 which was isolated from zygotic embryos of B. napus, but were 54 amino acids shorter than Lea 76. Acquisition of desiccation tolerance of the microspore-derived embryos and accumulation of mRNA and its transcripts of the Lea genes began within 12 h of ABA treatment. The embryos treated with ABA for 48-168 h showed a high desiccation tolerance and accumulation of Lea proteins at high levels. The close relationships between the induction of desiccation tolerance of embryos and the expression level of Lea genes suggest an important function of Lea proteins for desiccation tolerance in embryos.

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