A dual enzyme-phosphate hybrid nanoflower for glutamate detection

Li, Peikun; Jia, Jiahui; Geng, Zixin; Pang, Saizhao; Wang, Ruirui; Bilal, Muhammad; Bian, Hongjie; Cui, Jiandong; Jia, Shiru

doi:10.1016/j.partic.2023.02.008

Cited by 22 publications

(9 citation statements)

References 33 publications

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“…14,15 Compared to free enzymes, the asprepared HNFs exhibit significantly enhanced activity and stability and can be easily separated from the reaction system for recycling. 16,17 The efficient enzymatic catalytic performance of HNFs is mainly attributed to two reasons: one is that HNFs are flower-like structures composed of multiple layers, which is more conducive to macromolecular diffusion and substrate transport; second, the Fenton-like effect of Cu 2+ and the Cu 2+ activation effect of enzyme molecules, which has been confirmed in our previous reports. 18 Such an activation effect of metal cations on enzymes was confirmed by studying the properties of CaHPO 4 -α-amylase and FeP 4 -horseradish peroxidase (HRP) HNFs.…”

Section: ■ Introductionsupporting

confidence: 72%

“…In 2012, Zare and co-workers first reported a simple organic–inorganic hybrid nanoflower (HNF) technology. HNFs are three-dimensional flower-like nanospheres formed by laccase and copper phosphate crystals through biomineralization. , Compared to free enzymes, the as-prepared HNFs exhibit significantly enhanced activity and stability and can be easily separated from the reaction system for recycling. , The efficient enzymatic catalytic performance of HNFs is mainly attributed to two reasons: one is that HNFs are flower-like structures composed of multiple layers, which is more conducive to macromolecular diffusion and substrate transport; second, the Fenton-like effect of Cu 2+ and the Cu 2+ activation effect of enzyme molecules, which has been confirmed in our previous reports . Such an activation effect of metal cations on enzymes was confirmed by studying the properties of CaHPO 4 -α-amylase and FeP 4 -horseradish peroxidase (HRP) HNFs. , The α-amylase and HRP are metalloenzymes with active centers containing calcium and iron ions, and the introduction of Ca 2+ and Fe 2+ can interact with the amino acid residues of the enzyme, which can lead to activation of the enzyme.…”

Section: Introductionmentioning

confidence: 99%

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Controllable Synthesis of Hemoglobin–Metal Phosphate Organic–Inorganic Hybrid Nanoflowers and Their Applications in Biocatalysis

2023

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In recent years, organic–inorganic hybrid nanoflower technology has become an effective method for enzyme immobilization. Here, seven hierarchical flower-like hemoglobin-phosphate organic-inorganic hybrid nanomaterials (Hb-M3(PO4)2 ·nH2O HNFs) were synthesized through an improved universal one-pot wet-chemical method, with Ca2+, Mn2+, Fe2+, Co2+, Ni2+, Cu2+ and Zn2+ as inorganic components. In this synthesis process, the metal cations are successively involved in the coordination reaction with Hb and the metathesis reaction to generate phosphate precipitation. The coordination ability of metal cations and the generation rate of phosphate precipitations were evaluated, then the progress of the two chemical reactions was controlled synchronously by adjusting the phosphate buffer (PB) concentration, and finally a flower-like structure conducive to substrate diffusion and transport was obtained. Due to the conformational transformation of hemoglobin and the abundant Cu2+/Fe3+ active sites, the hemoglobin-Cu3(PO4)2·3H2O nanoflowers have extremely high catalytic activity, which is ∼14 times that of Hb. Importantly, this method is suitable for the monometallic-ionic, polymetallic-ionic and polyvalent metal-ion nanoflowers, which broadens the chemical composition and structural diversity of nanoflowers.

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Section: ■ Introductionsupporting

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Controllable Synthesis of Hemoglobin–Metal Phosphate Organic–Inorganic Hybrid Nanoflowers and Their Applications in Biocatalysis

2023

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“…In another study, Sharma et al fabricated an immunosensor using a gold–chitosan nanocomposite to immobilize the antibody against MSG [ 14 ]. Moreover, a GluO x -based hybrid nanoflower and horseradish peroxidase were successfully prepared to design an MSG biosensor [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Development of an Enzymatic Biosensor Using Glutamate Oxidase on Organic–Inorganic-Structured, Electrospun Nanofiber-Modified Electrodes for Monosodium Glutamate Detection

et al. 2023

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Herein, dendrimer-modified montmorillonite (Mt)-decorated poly-Ɛ-caprolactone (PCL) and chitosan (CHIT)-based nanofibers were prepared. Mt was modified with a poly(amidoamine) generation 1 (PAMAMG1) dendrimer, and the obtained PAMAMG1–Mt was incorporated into the PCL–CHIT nanofiber’s structure. The PCL–CHIT/PAMAMG1–Mt nanofibers were conjugated with glutamate oxidase (GluOx) to design a bio-based detection system for monosodium glutamate (MSG). PAMAMG1–Mt was added to the PCL–CHIT backbone to provide a multipoint binding side to immobilize GluOx via covalent bonds. After the characterization of PCL–CHIT/PAMAMG1–Mt/GluOx, it was calibrated for MSG. The linear ranges were determined from 0.025 to 0.25 mM MSG using PCL–CHIT/Mt/GluOx and from 0.0025 to 0.175 mM MSG using PCL–CHIT/PAMAMG1–Mt/GluOx (with a detection limit of 7.019 µM for PCL–CHIT/Mt/GluOx and 1.045 µM for PCL–CHIT/PAMAMG1–Mt/GluOx). Finally, PCL–CHIT/PAMAMG1–Mt/GluOx was applied to analyze MSG content in tomato soup without interfering with the sample matrix, giving a recovery percentage of 103.125%. Hence, the nanofiber modification with dendrimer-intercalated Mt and GluOx conjugation onto the formed nanocomposite structures was performed, and the PCL–CHIT/PAMAMG1–Mt/GluOx system was successfully developed for MSG detection.

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“…Among the various enzyme nanoflowers studied, there has been a significant focus on single-enzyme HNFs, with enzymes such as laccase [49], lipase [50], horseradish peroxidase (HRP) [51], glucose oxidase (GOx) [52], α-amylase [53], urease (Ur) [54], and papain [55] being successfully prepared in the form of single-enzyme HNFs [3] (see Table 1). Galactose oxidase and horseradish peroxidase Mn 2+ Detection of glutamic acid Enhanced reusability [65] Horseradish peroxidase and glucose oxidase Cu 2+ Degradation of acridine and wastewater treatment Enhanced pH stability [66] Nucleoside kinase and polyphosphate kinase Cu 2+ Generation of nucleotides Enhanced reusability [67] Glucose oxidase and lipase Cu 2+ Epoxidation of alkenes Enhanced reusability [68] Streptavidin and horseradish peroxidase Cu 2+…”

Section: Conventional Hnfs Immobilized Enzymesmentioning

confidence: 99%

Recent Advances in the Strategies of Simultaneous Enzyme Immobilization Accompanied by Nanocarrier Synthesis

Hao,

Liu,

Chu

2024

Applied Sciences

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In recent years, with advancements in nanotechnology and materials science, new enzyme immobilization strategies based on nanomaterials have continuously emerged. These strategies have shown significant effects on enhancing enzyme catalytic performance and stability due to their high surface area, good chemical stability, and ease of enzyme binding, demonstrating tremendous potential for industrial applications. Those methods that can rapidly synthesize nanocarriers under mild conditions allow for the one-step synthesis of nanocarriers and enzyme complexes, thereby exhibiting advantages such as simplicity of process, minimal enzyme damage, short processing times, and environmental friendliness. This paper provides an overview of simultaneous enzyme immobilization strategies accompanied by nanocarrier synthesis, including organic–inorganic hybrid nano-flowers (HNFs), metal–organic frameworks (MOFs), and conductive polymers (CPs). It covers their preparation principles, post-immobilization performance, applications, and existing challenges.

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A dual enzyme-phosphate hybrid nanoflower for glutamate detection

Cited by 22 publications

References 33 publications

Controllable Synthesis of Hemoglobin–Metal Phosphate Organic–Inorganic Hybrid Nanoflowers and Their Applications in Biocatalysis

Controllable Synthesis of Hemoglobin–Metal Phosphate Organic–Inorganic Hybrid Nanoflowers and Their Applications in Biocatalysis

Development of an Enzymatic Biosensor Using Glutamate Oxidase on Organic–Inorganic-Structured, Electrospun Nanofiber-Modified Electrodes for Monosodium Glutamate Detection

Recent Advances in the Strategies of Simultaneous Enzyme Immobilization Accompanied by Nanocarrier Synthesis

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