A dual-functional lanthanide nanoprobe for both living cell imaging and ICP-MS quantification of active protease

Duan, Feng; Tian, Fang; Qin, Weijie; Qian, Xiaohong

doi:10.1039/c5sc03363b

Cited by 36 publications

(22 citation statements)

References 48 publications

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“…Some works have been published about the bimodal detection concept using fluorescence and ICP-MS [8,[27][28][29]. Bustos et al [8] reported a comparison of both detection modes with CdSe/ZnS QDs through the measurement of Cd in the case of ICP-MS. Other examples include the use of a nanoprobe composed of europium chelated by 1,10-bis(50-chlorosulfo-thiophene-20-yl)-4,4,5,5,6,6,7,7-octafluorodecane-1,3,8,10-tetraone [28], the synthesis of hybrid labels containing both a DTPA chelate (coordinated with either 165 Ho or 111 In) and a Cy5 fluorescent dye [27], or by combining a fluorescent dye (Cy3) with upconversion NPs [29]. Those probes are compose either of just one metal atom or of atoms of different heteroatoms (e.g.…”

Section: Introductionmentioning

confidence: 99%

Bimodal determination of immunoglobulin E by fluorometry and ICP-MS by using platinum nanoclusters as a label in an immunoassay

et al. 2019

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The authors describe the use of platinum nanoclusters (PtNCs) as bimodal labels in a competitive immunoassay for immunoglobulin E (IgE). Both fluorometry and inductively coupled plasmamass spectrometry (ICP-MS) are used. Optimization of the PtNCs synthesis process using lipoic acid as ligand was carried out. The time for synthesis and the effect of NaOH added to the PtNCs precursor mixture was optimized with the aim to obtain PtNCs with strong fluorescence and low size dispersity. Maximal fluorescence was obtained at excitation/emission wavelengths of 455/620 nm. The average diameter (1.5 nm) and crystal structure (face-centered cubic structure) of the PtNCs were determined by HR-TEM. It was calculated that each PtNC contains 116 Pt atoms at average. Labelling of the antibody (Ab) against IgE with PtNCs was optimized in terms of recognition capabilities and fluorescence signal. A molar ratio (Ab:PtNCs) of 1:11 is found to be best. A competitive immunoassay for IgE was developed and detection was carried out by using both ICP-MS (by measuring 195 Pt) and fluorometry. The limit of detection (LOD) of the fluoroimmunoassay is 0.6 ng mL-1 of IgE. The LOD of the ICP-MS method is as low as 0.08 ng mL-1. The method was evaluated by analyzing four (spiked) serum samples by ICP-MS. No sample pretreatment excepting dilution is needed. Results compared favorably with those obtained by a commercial ELISA kit.

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Section: Introductionmentioning

confidence: 99%

Bimodal determination of immunoglobulin E by fluorometry and ICP-MS by using platinum nanoclusters as a label in an immunoassay

et al. 2019

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“…This LOD might be improved using a lanthanidecontaining optical and mass signals affordable molecule, for example, Eu-BCTOT for fluorescent imaging and ICPMS quantification because of the luminescent property of the Eucomplex. 35 The dynamic range of the Sias number against 10 B ICPMS intensity was from 10 −13 to 10 −8 mol Sia (R = 0.9990, RSD = 5.5% at 10 −9 level, n = 5) (Figure 3c).…”

mentioning

confidence: 96%

“…In total, 1.41 × 10 11 Sias (2.35 × 10 –13 mol) per SMMC-7721cell (RSD = 5.9%, n = 5) and 4.71 × 10 8 Sias (7.81 × 10 –16 mol) per LO2 cell (RSD = 6.0%, n = 5) were quantified with 0.24 pmol LOD (3σ) of B-tagged Sias, which were well in agreement with those using DBCO-DOTA-Eu, although the LOD (3σ) was almost 27-times higher. This LOD might be improved using a lanthanide-containing optical and mass signals affordable molecule, for example, Eu-BCTOT for fluorescent imaging and ICPMS quantification because of the luminescent property of the Eu-complex . The dynamic range of the Sias number against 10 B ICPMS intensity was from 10 –13 to 10 –8 mol Sia ( R = 0.9990, RSD = 5.5% at 10 –9 level, n = 5) (Figure c).…”

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confidence: 99%

Metabolism-Based Click-Mediated Platform for Specific Imaging and Quantification of Cell Surface Sialic Acids

et al. 2016

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Although we believe that the cell surface sialic acids (Sias) are playing an important role in cell-cell interactions and related tumor metastasis processes, acquisition of their quantitative information has yet been a challenge to date. Here, we reported the construction of a new analytical platform for Sias-specific imaging and quantification. We used N-azidoacetyl-mannosamine tetraacylated as a metabolic sugar substrate to bioassemble azido-Sias on the surface of cells via the metabolic pathway of Sias de novo synthesis. These azido-Sias allow us to perform a duplex Sias-specific analysis with various fluorescent and elemental reporters such as DIBO-Alexa Fluor 647, DBCO-DOTA-Eu, and DBCO-PEG-BODIPY, which can be easily labeled and/or tagged through an effective copper-free bioorthogonal click reaction. Compared to the previous reported strategies, we quantified the cell surface Sias with the LODs (3σ) down to 8.9 fmol and 0.24 pmol using Eu- andB-species unspecific isotope dilution ICPMS, in addition to their red- and green-CLSM profiling. Such a platform enables us to evaluate Sias regulation under the administration of paclitaxel, finding that 1 μM paclitaxel induced a significant Sias decrease of 67% on the surface of hepatic tumor cell SMMC-7721, while had no obvious adverse effect to that of para-carcinomatous liver cell LO2. Besides Sias, we believe that this metabolism-based click-mediated platform will provide opportunities to study other monosaccharides and their corresponding biological roles when more corresponding chemically modified sugar substrates and specific bioorthogonal reactions are developed.

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“…Besides outstanding luminescent properties, the state-of-the-art fluorescent materials, involving metal–organic frameworks, quantum dots, noble metal nanoclusters, and upconversion lanthanides nanoparticles, often intrinsically contain a great number of metal stable isotopes. , The metal stable isotopes can be efficiently quantified by ICPMS. Consequently, bimodal detection is potentially available by utilizing both fluorescence spectrometry and mass spectrometry, which would integrate the advantages of the two analytical techniques and may promote our understanding on explosive monitoring processes.…”

mentioning

confidence: 99%

“…18,19 The metal stable isotopes can be efficiently quantified by ICPMS. Consequently, bimodal detection is potentially available by utilizing both fluorescence spectrometry and mass spectrometry, 20 which would integrate the advantages of the two analytical techniques and may promote our understanding on explosive monitoring processes. Herein, we report the design and construction of a bimodal methodology for both sensitive in-field fluorescence detection and accurate laboratory mass spectrometric quantification of explosives by taking advantage of the characteristic fluorescence and ICPMS response of copper nanoparticles (CuNPs).…”

mentioning

confidence: 99%

Poly(thymine)-CuNPs: Bimodal Methodology for Accurate and Selective Detection of TNT at Sub-PPT Levels

Wang

Liu

et al. 2018

Anal. Chem.

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Accurate, sensitive, and selective detection of explosives is of vital importance in antiterrorism and homeland security. Fluorescence sensors are prevalent for sensitive and fast in-field explosive detection but are sometimes compromised by accuracy and stability due to the similar structures of explosives, photobleaching, and complex sample matrixes. Herein, we developed a first bimodal methodology capable of both sensitive in-field fluorescence detection and accurate laboratory mass spectrometric quantification of 2,4,6-trinitrotoluene (TNT) by utilizing the characteristic fluorescent and mass spectrometric response of copper nanoparticles (CuNPs). An excellent selectivity was also realized by involving aptamer recognition. The methodology is capable of detecting TNT at subpart per trillion (PPT) levels, with a detection limit of 0.32 pg mL −1 by inductively coupled plasma mass spectrometry (ICPMS) and 0.17 ng mL −1 by fluorimetry. The signal response was accurate and stable for at least 60 days by ICPMS. Thanks to the biospecificity of the aptamer, this bimodal methodology is potentially applicable to a large panel of explosives.

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A dual-functional lanthanide nanoprobe for both living cell imaging and ICP-MS quantification of active protease

Abstract: A novel nanoprobe is successfully developed for simultaneous living cell imaging and sensitive quantification of caspase-3 in cancer cells.

Cited by 36 publications

References 48 publications

Bimodal determination of immunoglobulin E by fluorometry and ICP-MS by using platinum nanoclusters as a label in an immunoassay

Bimodal determination of immunoglobulin E by fluorometry and ICP-MS by using platinum nanoclusters as a label in an immunoassay

Metabolism-Based Click-Mediated Platform for Specific Imaging and Quantification of Cell Surface Sialic Acids

Poly(thymine)-CuNPs: Bimodal Methodology for Accurate and Selective Detection of TNT at Sub-PPT Levels

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