A dual marker label free electrochemical assay for Flavivirus dengue diagnosis

Santos, Alexandre Leme Godoy dos; Bueno, Paulo Agenor Alves; Davis, Jason J.

doi:10.1016/j.bios.2017.09.014

Cited by 48 publications

(23 citation statements)

References 53 publications

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“…100 Hz optimized frequency was found, and the target-responsive data is shown in Fig. 2 E ( Santos et al, 2018 ). Nguyen et al used serotype 2 (Denv2) as an analyte in the EIS system and explored that resistance and capacitance are effective to detect Denv2.…”

Section: Electrochemical Biosensors For Virus Detectionmentioning

confidence: 96%

“…2 (A–D) Schematic construction of the anti-NS1 and NS1 modified electrode surfaces, (E) Example of 1/C″ immittance function investigation for NS1 detection in PBS, the optimized frequency, 100 Hz, corresponds to the most sensitive response with R 2 > 0.96. Adapted from ( Santos et al, 2018 ). (F) Consecutive experimental steps using the same nano biosensor, investigated after 30 min incubations of CHIKV, WNV, and DENV-2 respectively.…”

Section: Electrochemical Biosensors For Virus Detectionmentioning

confidence: 99%

See 1 more Smart Citation

Ultrasensitive detection of pathogenic viruses with electrochemical biosensor: State of the art

Khan¹,

Hasan

Hossain

et al. 2020

Biosensors and Bioelectronics

174

108

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Last few decades, viruses are a real menace to human safety. Therefore, the rapid identification of viruses should be one of the best ways to prevent an outbreak and important implications for medical healthcare. The recent outbreak of coronavirus disease (COVID-19) is an infectious disease caused by a newly discovered coronavirus which belongs to the single-stranded, positive-strand RNA viruses. The pandemic dimension spread of COVID-19 poses a severe threat to the health and lives of seven billion people worldwide. There is a growing urgency worldwide to establish a point-of-care device for the rapid detection of COVID-19 to prevent subsequent secondary spread. Therefore, the need for sensitive, selective, and rapid diagnostic devices plays a vital role in selecting appropriate treatments and to prevent the epidemics. During the last decade, electrochemical biosensors have emerged as reliable analytical devices and represent a new promising tool for the detection of different pathogenic viruses. This review summarizes the state of the art of different virus detection with currently available electrochemical detection methods. Moreover, this review discusses different fabrication techniques, detection principles, and applications of various virus biosensors. Future research also looks at the use of electrochemical biosensors regarding a potential detection kit for the rapid identification of the COVID-19.

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Section: Electrochemical Biosensors For Virus Detectionmentioning

confidence: 96%

Section: Electrochemical Biosensors For Virus Detectionmentioning

confidence: 99%

Ultrasensitive detection of pathogenic viruses with electrochemical biosensor: State of the art

Khan¹,

Hasan

Hossain

et al. 2020

Biosensors and Bioelectronics

174

108

View full text Add to dashboard Cite

show abstract

“…Typically, the obtained impedance spectra are fitted to an equivalent circuit using a Nyquist plot for illustration, and the change in charge transfer resistance is correlated to the target biological material concentration [62]. For the situation of an electrode in contact with an electrolyte, the so-called Randles circuit is used (Figure 1), comprising the double-layer capacitance C dl, the solution resistance R s , the charge transfer resistance R ct , and the Warburg impedance W. (measuring the conductivity or resistance) [44], amperometry/voltammetry (current measurement as a function of imposed potential) [28,[45][46][47], and EIS (measuring the impedance of a system) [48][49][50]. The majority of publications over the past decades focus on voltammetric and EIS techniques to obtain label-free biosensors with a high sensitivity and, therefore, low limit of detection (LOD), which are the main figures of merit of biosensors.…”

Section: Electrochemical Impedance Spectroscopy and Conductometrymentioning

confidence: 99%

“…The majority of publications are devoted to registration of the resultant current of the redox probe corresponding to changing electrode potential. Ferri/ferrocyanide, ferrocene, ferrocenethanol systems, and ruthenium complexes are the most frequently used redox probes [50,69,70]. The electrochemical behavior of redox probes depends on the receptor layer and the biosensor operating principle.…”

Section: Voltammetry Amperometrymentioning

confidence: 99%

Label-Free Electrochemical Biosensors for the Determination of Flaviviruses: Dengue, Zika, and Japanese Encephalitis

Khristunova

Dorozhko

Короткова

et al. 2020

Sensors

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A highly effective way to improve prognosis of viral infectious diseases and to determine the outcome of infection is early, fast, simple, and efficient diagnosis of viral pathogens in biological fluids. Among a wide range of viral pathogens, Flaviviruses attract a special attention. Flavivirus genus includes more than 70 viruses, the most familiar being dengue virus (DENV), Zika virus (ZIKV), and Japanese encephalitis virus (JEV). Haemorrhagic and encephalitis diseases are the most common severe consequences of flaviviral infection. Currently, increasing attention is being paid to the development of electrochemical immunological methods for the determination of Flaviviruses. This review critically compares and evaluates recent research progress in electrochemical biosensing of DENV, ZIKV, and JEV without labelling. Specific attention is paid to comparison of detection strategies, electrode materials, and analytical characteristics. The potential of so far developed biosensors is discussed together with an outlook for further development in this field.

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“…Aptamers are oligonucleotide or protein recognition probes that are selected for binding a target molecule from a large random sequence pool. Davis’ group has done interesting work on EIS-based label-free immunoassays that have catalyzed progress in unlabeled immunoassays and can be adapted to printed electrodes [ 116 , 117 , 118 , 119 , 120 , 121 ].…”

Section: Immunoassay Techniques For Printed Electrodesmentioning

confidence: 99%

Printed Electrodes in Microfluidic Arrays for Cancer Biomarker Protein Detection

et al. 2020

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Medical diagnostics is trending towards a more personalized future approach in which multiple tests can be digitized into patient records. In cancer diagnostics, patients can be tested for individual protein and genomic biomarkers that detect cancers at very early stages and also be used to monitor cancer progression or remission during therapy. These data can then be incorporated into patient records that could be easily accessed on a cell phone by a health care professional or the patients themselves on demand. Data on protein biomarkers have a large potential to be measured in point-of-care devices, particularly diagnostic panels that could provide a continually updated, personalized record of a disease like cancer. Electrochemical immunoassays have been popular among protein detection methods due to their inherent high sensitivity and ease of coupling with screen-printed and inkjet-printed electrodes. Integrated chips featuring these kinds of electrodes can be built at low cost and designed for ease of automation. Enzyme-linked immunosorbent assay (ELISA) features are adopted in most of these ultrasensitive detection systems, with microfluidics allowing easy manipulation and good fluid dynamics to deliver reagents and detect the desired proteins. Several of these ultrasensitive systems have detected biomarker panels ranging from four to eight proteins, which in many cases when a specific cancer is suspected may be sufficient. However, a grand challenge lies in engineering microfluidic-printed electrode devices for the simultaneous detection of larger protein panels (e.g., 50–100) that could be used to test for many types of cancers, as well as other diseases for truly personalized care.

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A dual marker label free electrochemical assay for Flavivirus dengue diagnosis

Cited by 48 publications

References 53 publications

Ultrasensitive detection of pathogenic viruses with electrochemical biosensor: State of the art

Ultrasensitive detection of pathogenic viruses with electrochemical biosensor: State of the art

Label-Free Electrochemical Biosensors for the Determination of Flaviviruses: Dengue, Zika, and Japanese Encephalitis

Printed Electrodes in Microfluidic Arrays for Cancer Biomarker Protein Detection

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