A dual role for H2A.Z.1 in modulating the dynamics of RNA Polymerase II initiation and elongation

Mylonas, Constantine; Auld, Alexander L.; Lee, Choongman; Cissé, Ibrahima; Boyer, Laurie A.

doi:10.1101/2020.06.22.165373

2020

DOI: 10.1101/2020.06.22.165373

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A dual role for H2A.Z.1 in modulating the dynamics of RNA Polymerase II initiation and elongation

Constantine Mylonas

Alexander L. Auld

Choongman Lee

et al.

Abstract: 15RNAPII pausing immediately downstream of the transcription start site (TSS) is a critical rate 16 limiting step at most metazoan genes that allows fine-tuning of gene expression in response to 17 diverse signals 1-5 . During pause-release, RNA Polymerase II (RNAPII) encounters an H2A.Z.

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Newly synthesized RNA Sequencing Characterizes Transcription Dynamics in Three Pluripotent States

Shao

Kumar

Liedschreiber

et al. 2021

Preprint

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Unique transcriptomes define naïve, primed and paused pluripotent states in mouse embryonic stem cells. Here we perform transient transcriptome sequencing (TT-seq) to de novo define and quantify coding and non-coding transcription units (TUs) in different pluripotent states. We observe a global reduction of RNA synthesis, total RNA amount and turnover rates in ground state naïve cells (2i) and paused pluripotency (mTORi). We demonstrate that elongation velocity can be reliably estimated from TT-seq nascent RNA and RNA polymerase II occupancy and observe a transcriptome-wide attenuation of elongation velocity in the two inhibitor-induced states. We also discover a relationship between elongation velocity and termination read-through distance. Our analysis suggests that steady-state transcriptomes in mouse ES cells are controlled predominantly on the level of RNA synthesis, and that signaling pathways governing different pluripotent states immediately control key parameters of transcription.

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Newly synthesized RNA Sequencing Characterizes Transcription Dynamics in Three Pluripotent States

Shao

Kumar

Liedschreiber

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

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A dual role for H2A.Z.1 in modulating the dynamics of RNA Polymerase II initiation and elongation

Abstract: 15RNAPII pausing immediately downstream of the transcription start site (TSS) is a critical rate 16 limiting step at most metazoan genes that allows fine-tuning of gene expression in response to 17 diverse signals 1-5 . During pause-release, RNA Polymerase II (RNAPII) encounters an H2A.Z.

Cited by 1 publication

References 64 publications

Newly synthesized RNA Sequencing Characterizes Transcription Dynamics in Three Pluripotent States

Newly synthesized RNA Sequencing Characterizes Transcription Dynamics in Three Pluripotent States

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