A dynamic atlas of immunocyte migration from the gut

Abstract

Dysbiosis in the gut microbiota impacts several systemic diseases. One possible mechanism is the migration of perturbed intestinal immunocytes to extra-intestinal tissues. Combining the Kaede photoconvertible mouse model and single-cell genomics, we generated a detailed map of migratory trajectories from the colon, at baseline and during intestinal and extra-intestinal inflammation. All colonic lineages emigrated from the colon in an S1P-dependent manner, dominated by B lymphocytes with a large continuous circulation of follicular B cells, which carried a gut-imprinted transcriptomic signature. T cell emigration was more selective, with distinct groups of RORγ+or IEL-like CD160+cells in the spleen. Gut inflammation curtailed emigration, except for DCs disseminating to lymph nodes. Colon emigrating cells distributed differentially to tumor, skin inflammation, or arthritic synovium, the former dominated by myeloid cells in a chemokine-dependent manner. These results thus reveal specific cellular trails originating in the gut, influenced by microbiota, which can shape peripheral immunity.

Single-cell transcriptomics reveals colonic immune perturbations during amyloid-β driven Alzheimer’s disease in mice

“…2A, S3B). A similar expression signature was recently reported in Kaede mice including Cxcr4, Itgb7, Gpr174 and, transiently AP-1 factor, Jun, which defined a gut-imprinted transcriptomic signature in colon B cell emigrants in the spleen under homeostatic conditions [53]. Isolated to the receding cluster 5, we also found Klf family genes ( Klf2, Klf4, Klf5, Klf6 ) (Fig.…”

“…1H, S4A-B). Although this did not rule out stress-induced apoptosis of any short-lived PBs within cluster 5, an abundance of migratory signatures [53] within this cluster and previous reports of neuroinflammation driven gut-brain PC migration [27,54] support plausible colon PB emigration in the 5XFAD model.…”

The gut microbiota in alopecia areata: Potential predictive biomarkers and therapy targets?

“…Imbalanced immune tolerance, an abundance of antigen profiles, abnormal activation of immune cells, and metabolism disorders in the gut have been strongly linked to autoimmune diseases. The updated investigation demonstrated that immunocytes from the colon can migrate into extraintestinal tissues, depending on sphingosine‐1‐phosphate (S1P), and could be disrupted by antibiotics 4 . The distribution pattern of gut immunocytes significantly varies in different inflammatory organs 4 .…”

“…The updated investigation demonstrated that immunocytes from the colon can migrate into extraintestinal tissues, depending on sphingosine‐1‐phosphate (S1P), and could be disrupted by antibiotics 4 . The distribution pattern of gut immunocytes significantly varies in different inflammatory organs 4 . Furthermore, certain gut microbiota were widely identified as potential biomarkers for early diagnosis, disease progression, or intervention targets in autoimmune diseases and cancer.…”

“…4 The distribution pattern of gut immunocytes significantly varies in different inflammatory organs. 4 Furthermore, certain gut microbiota were widely identified as potential biomarkers for early diagnosis, disease progression, or intervention targets in autoimmune diseases and cancer.…”