A Dynamic Defect Generation Strategy for Efficient Enzyme Immobilization in Robust Metal‐Organic Frameworks for Catalytic Hydrolysis and Chiral Resolution

Abstract

Enzyme immobilization has been demonstrated to be a favorable protocol for promoting the industrialization of bioactive molecules, but still with formidable challenge. Addressing this challenge, we create a dynamic defect generation strategy for enzyme immobilization by using the dissociation equilibrium of metal‐organic frameworks (MOFs) mediated by enzymes. Enzymes can act as “macro ligands” to generate competitive coordination against original ligands, along with the release of metal clusters of MOFs to generate defects, hence promoting the gradual transport of enzymes from the surface to inside. Various enzymes can be efficiently immobilized in MOFs to afford composites with good enzymatic activities, protective performances and exceptional reusabilities. Moreover, multienzyme bioreactors capable of efficient cascade reactions can also be generated. This study provides new opportunities to construct highly efficient biocatalysts incorporating different types of enzymes.

Bioprocessos: Processos Fermentativos Para Produção De Enzimas E Imobilização Para Aplicação Industrial

“…Processos com enzimas imobilizadas em biorreatores permitem uma rápida recuperação dos produtos e das enzimas, onde elas podem ser reutilizadas nos processos enzimáticos contínuos e uma gama mais ampla de designs. Os biorreatores apresentam um papel crucial na produção industrial, por permitirem o cultivo controlado de microrganismos ou de células geneticamente modificadas (BORNFELT, 2023).…”

Defect-containing metal–organic framework materials for sensor applications

“…However, conventional carrier materials might encounter limitations in certain scenarios, where in this interdisciplinary field, defect-engineered MOFs are emerging as a novel class of materials. In a related study, Feng et al 110 present an innovative dynamic strategy for enzyme immobilization within MOFs to address challenges in industrializing bioactive molecules. This approach leverages enzyme-mediated MOF dissociation, with enzymes acting as “macro ligands,” competing with original ligands.…”

Hollow Hierarchical Porous and Antihydrolytic Spherical Zeolitic Imidazolate Frameworks for Enzyme Encapsulation and Biocatalysis

“…Metal−organic frameworks (MOFs) are a class of porous materials that are self-assembled from metal ions or metal clusters with organic ligands. 3 Due to their diverse topologies and properties, ultrahigh specific surface area, abundant and easily adjustable pore structure, and good stability, MOFs have received much attention in the fields of gas adsorption, biomedicine, biosensing, and enzyme immobilization catalysis. 4 Zeolitic imidazolate frameworks (ZIFs), a subfamily of MOFs, are porous crystalline materials with extended threedimensional (3D) structures formed by the coordination of transition metal ions with imidazole derivative ligands, which have been widely reported as an ideal platform for enzyme immobilization due to its mild synthesis conditions and biocompatibility.…”

“…Metal–organic frameworks (MOFs) are a class of porous materials that are self-assembled from metal ions or metal clusters with organic ligands . Due to their diverse topologies and properties, ultrahigh specific surface area, abundant and easily adjustable pore structure, and good stability, MOFs have received much attention in the fields of gas adsorption, biomedicine, biosensing, and enzyme immobilization catalysis .…”