A dynamic flow‐chamber‐based adhesion assay to assess canine platelet–matrix interactions in vitro

Ferkau, Annika; Ecklebe, Silke; Jahn, Kirsten; Calmer, Simone; Theilmeier, Gregor; Mischke, R.

doi:10.1111/vcp.12035

Cited by 3 publications

(2 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Platelet adhesion assays, based on platelet properties to adhere to specific extracellular matrix components, represent another platelet function testing modality used primarily in platelet function research [132]. In the evaluation of platelet adhesion, an advanced method as the flow chamber has been shown to have some use in dogs [133].…”

Section: Platelet Laboratory Testing In Dogsmentioning

confidence: 99%

Platelet Function and Therapeutic Applications in Dogs: Current Status and Future Prospects

Cortese

Christopherson

Pelagalli

2020

Animals

View full text Add to dashboard Cite

Significant progress has been made in the functional characterization of canine platelets in the last two decades. The role of canine platelets in hemostasis includes their adhesion to the subendothelium, activation, and aggregation, leading to primary clot formation at the site of injury. Studies on canine platelet function and advancements in laboratory testing have improved the diagnosis and understanding of platelet-related disorders as well as the knowledge of the mechanisms behind these diseases. This review focuses on the most recent discoveries in canine platelet structure, function, and disorders; and discusses the efficacy of various tests in the diagnosis of platelet-related disorders. With the relatively recent discovery of angiogenetic and reparative effects of growth factors found in platelets, this review also summarizes the use of canine platelet-rich plasma (PRP) alone or in association with stem cells in regenerative therapy. The characterization of proteomic and lipidomic profiles and development of platelet gene therapy in veterinary species are areas of future study with potential for major therapeutic benefits.

show abstract

Section: Platelet Laboratory Testing In Dogsmentioning

confidence: 99%

Platelet Function and Therapeutic Applications in Dogs: Current Status and Future Prospects

Cortese

Christopherson

Pelagalli

2020

Animals

View full text Add to dashboard Cite

show abstract

“…Previous studies in dogs have used flow chamber assays to evaluate platelet interactions with extracellular matrix components (53) and to assess the influence of bacterial infection, lipopolysaccharide and hydroxyethyl starch on platelet function (54). Flow chamber systems have also been used to study human patients with hemophilia, VWD and hereditary platelet function disorders including May-Hegglin anomaly, gray platelet syndrome, and Glanzmann thrombasthenia (55), all of which are disorders of relevance to canine medicine.…”

Section: Figure 3 |mentioning

confidence: 99%

A Novel Microchip Flow Chamber (Total Thrombus Analysis System) to Assess Canine Hemostasis

et al. 2020

View full text Add to dashboard Cite

Hemorrhagic diseases are common in dogs. Current coagulation assays do not model all aspects of in vivo hemostasis and may not predict bleeding risk. The Total-Thrombus Analysis System (T-TAS) is a novel hemostasis assay system in which whole blood flows through microfluidic channels at defined shear rates to provide qualitative and quantitative evaluation of platelet function (PL-chip) and coagulation function (AR-chip). The present study evaluated the T-TAS in dogs with hereditary bleeding disorders and with acquired hemorrhagic syndromes (Group 1), and healthy controls (Group 2). Hereditary defects included von Willebrand's disease (VWD; n = 4), hemophilia A (n = 2), and canine Scott syndrome (n = 2). Acquired hemorrhagic disorders included neoplastic hemoperitoneum (n = 2) and acute hemorrhagic diarrhea syndrome (n = 1). Citrate anticoagulated samples were collected from diseased dogs (Group 1, n = 11) and controls (Group 2, n = 11) for coagulation screening tests, fibrinogen analyses, D-dimer concentration, antithrombin activity, von Willebrand Factor antigen, PFA-100 closure time (PFA-CT), and thromboelastography (TEG). Citrate and hirudin anticoagulated samples were used for T-TAS analyses at two shear rates. Qualitative thrombus formation in each chip was recorded using the T-TAS video camera. Numeric parameters, derived from the instrument software, included occlusion start time (OST; time to 10 kPa), occlusion time (OT; time to 60 kPa (PL-chip) or 80 kPa (AR-chip)), and area under the pressure curve (AUC). Correlations between continuous variables were evaluated by Spearman's rank. Continuous variables were compared between groups by Student's t-test or the Mann-Whitney U-test. Alpha was set at 0.05. In combined analyses of all dogs, significant correlations were identified between T-TAS variables, between the PFA-CT and PL-chip parameters and between TEG variables and AR-chip parameters. The prothrombin time correlated with the AR-chip AUC at both shear rates. In Group 1 dogs, the AR-chip AUC at low shear was significantly reduced compared with Group 2 dogs. Aberrant thrombus formation was seen in video images recorded from dogs with VWD and hemophilia A. The T-TAS AR-chip analysis distinguished dogs with bleeding risk compared to healthy controls. Initial evaluations of the T-TAS suggest it may aid characterization of hemostasis in patients at-risk of bleeding and assist with delineating bleeding phenotypes.

show abstract

Effect of Clopidogrel on Thrombus Formation in an Ex Vivo Parallel Plate Flow Chamber Model Cannot Be Reversed by Addition of Platelet Concentrates or vWF Concentrate

Jahn

Suchodolski

Schäfer

et al. 2017

Anesthesia &Amp; Analgesia

View full text Add to dashboard Cite

show abstract

A dynamic flow‐chamber‐based adhesion assay to assess canine platelet–matrix interactions in vitro

Cited by 3 publications

References 17 publications

Platelet Function and Therapeutic Applications in Dogs: Current Status and Future Prospects

Platelet Function and Therapeutic Applications in Dogs: Current Status and Future Prospects

A Novel Microchip Flow Chamber (Total Thrombus Analysis System) to Assess Canine Hemostasis

Effect of Clopidogrel on Thrombus Formation in an Ex Vivo Parallel Plate Flow Chamber Model Cannot Be Reversed by Addition of Platelet Concentrates or vWF Concentrate

Contact Info

Product

Resources

About