A facile and sensitive method for detecting pathogenic bacteria using personal glucose meters

Joo, Jinmyoung; Kwon, Donghoon; Shin, Hwa Hui; Park, Ki-Hwan; Joon, Hyung; Jeon, Sangmin

doi:10.1016/j.snb.2013.08.027

Cited by 42 publications

(20 citation statements)

References 16 publications

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“…After washing away those components that do not bind to the micro-beads or micro-plates, the enzyme contained in the sandwich complex can be used to generate glucose and establish a direct correlation between the concentration of the target and the glucose monitored by a BGM. Using the sandwich format, a number of sensitive and selective BGM-based immunoassays have been developed for the detection of streptavidin and prostate-specific antigen (PSA) (Xiang and Lu, 2012a), insulin and glycated hemoglobin (HbA1c) (Xiang et al, 2014), alpha-fetoprotein (AFP) (Wu et al, 2015), carcinoembryonic antigen (CEA) (Su et al, 2012), phosphorylated p53 (on Serine 15, phospho-p53) (Zhao et al, 2015b), neuron-specic enolase (NSE) (Fu et al, 2014, Fu et al, 2015), albumin (Zhao et al, 2015a), and Salmonella (Joo et al, 2013). In some cases, additional signal amplification was provided by using nanomaterials, such as gold nanoparticles and nanoflowers (Fu et al, 2014, Fu et al, 2015), dendritic polyaniline nanofiber (DPANF) (Wu et al, 2015), and graphene-oxide-gold nanocomposite (Zhao et al, 2015a), to increase enzyme loading and hence higher sensitivity.…”

Section: Design Of the Bgm-based Biosensors For Ivds Of Non-glucosmentioning

confidence: 99%

Transforming the blood glucose meter into a general healthcare meter for in vitro diagnostics in mobile health

Lan

Zhang

2016

Biotechnology Advances

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Recent advances in mobile network and smartphones have provided an enormous opportunity for transforming in vitro diagnostics (IVD) from central labs to home or other points of care (POC). A major challenge to achieving the goal is a long time and high costs associated with developing POC IVD devices in mobile Health (mHealth). Instead of developing a new POC device for every new IVD target, we and others are taking advantage of decades of research, development, engineering and continuous improvement of the blood glucose meter (BGM), including those already integrated with smartphones, and transforming the BGM into a general healthcare meter for POC IVDs of a wide range of biomarkers, therapeutic drugs and other analytical targets. In this review, we summarize methods to transduce and amplify selective binding of targets by antibodies, DNA/RNA aptamers, DNAzyme/ribozymes and protein enzymes into signals such as glucose or NADH that can be measured by commercially available BGM, making it possible to adapt many clinical assays performed in central labs, such as immunoassays, aptamer/DNAzyme assays, molecular diagnostic assays, and enzymatic activity assays onto BGM platform for quantification of non-glucose targets for a wide variety of IVDs in mHealth.

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Section: Design Of the Bgm-based Biosensors For Ivds Of Non-glucosmentioning

confidence: 99%

Transforming the blood glucose meter into a general healthcare meter for in vitro diagnostics in mobile health

Lan

Zhang

2016

Biotechnology Advances

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“…270 Magnetic NP clusters are functionalized with APTES and GLD followed by the immobilization of monoclonal anti-Salmonella Abs. These clusters then bind to Salmonella in milk and are magnetically separated from solution using a magnet.…”

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confidence: 99%

Immobilization of Antibodies and Enzymes on 3-Aminopropyltriethoxysilane-Functionalized Bioanalytical Platforms for Biosensors and Diagnostics

et al. 2014

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“…The use of glucometers to monitor non-glucose targets was pioneered by Lu’s group, who adapted this device for aptamer- or DNAzyme-based detection of cocaine, ATP and metal ions 22 23 24 25 . These and other efforts 26 27 28 29 30 inspired our more generalizable approach to molecular diagnostics. The addition of a thermostable invertase allows all reactions to occur in the same tube at a wide temperature range, and the simplicity of the format ultimately yields atto-molar (or low zepto-mole) detection of pathogens such as MERS-CoV or Zaire Ebolavirus (ZEBOV or Zaire Ebola), even in sputum or saliva samples.…”

mentioning

confidence: 99%

A Sweet Spot for Molecular Diagnostics: Coupling Isothermal Amplification and Strand Exchange Circuits to Glucometers

Hughes

Bhadra

et al. 2015

Sci Rep

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Strand exchange nucleic acid circuitry can be used to transduce isothermal nucleic acid amplification products into signals that can be readable on an off-the-shelf glucometer. Loop-mediated isothermal amplification (LAMP) is limited by the accumulation of non-specific products, but nucleic acid circuitry can be used to probe and distinguish specific amplicons. By combining this high temperature isothermal amplification method with a thermostable invertase, we can directly transduce Middle-East respiratory syndrome coronavirus and Zaire Ebolavirus templates into glucose signals, with a sensitivity as low as 20–100 copies/μl, equating to atto-molar (or low zepto-mole). Virus from cell lysates and synthetic templates could be readily amplified and detected even in sputum or saliva. An OR gate that coordinately triggered on viral amplicons further guaranteed fail-safe virus detection. The method describes has potential for accelerating point-of-care applications, in that biological samples could be applied to a transducer that would then directly interface with an off-the-shelf, approved medical device.

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A facile and sensitive method for detecting pathogenic bacteria using personal glucose meters

Cited by 42 publications

References 16 publications

Transforming the blood glucose meter into a general healthcare meter for in vitro diagnostics in mobile health

Transforming the blood glucose meter into a general healthcare meter for in vitro diagnostics in mobile health

Immobilization of Antibodies and Enzymes on 3-Aminopropyltriethoxysilane-Functionalized Bioanalytical Platforms for Biosensors and Diagnostics

A Sweet Spot for Molecular Diagnostics: Coupling Isothermal Amplification and Strand Exchange Circuits to Glucometers

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