A facile electrochemical aptasensor for lysozyme detection based on target-induced turn-off of photosensitization

Chen, Zhaoxia; Xu, Qiao; Tang, Gangxu; Liu, Shuang; Xu, Shoufang; Zhang, Xinfeng

doi:10.1016/j.bios.2018.09.074

Cited by 28 publications

(14 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…First, the devices should feature miniaturized size and a high signal-noise ratio to monitor lysozyme concentration change in mobile and noisy scenarios. Lysozyme sensors based on different detection techniques, such as chromatography, immunoassay, electrochemical analysis, and optical methods, have been reported in the last decades [6][7][8][9]. Among them, Loncaric reported a USB-based electrochemical lysozyme sensor, which was composed of a potentiostat and I/V converter, an analog/digital converter, and a microcontroller unit [3].…”

Section: Introductionmentioning

confidence: 99%

Wireless Network of Silver Film Lysozyme Aptasensor Based on Fractal Measurement

Wang

2021

Security and Communication Networks

View full text Add to dashboard Cite

In this work, we reported a wireless network composed of silver film-based graphene oxide-fluorescence resonance energy transfer (GO-FRET) lysozyme aptasensor nodes. At the sensor node level, we optimized silver substrate structure, concentrations of the aptamers, and graphene oxide and tested lysozyme detection performance with a model analyte. At the network level, we analyzed the complexity and transmission success rate using fractal measurements. We implemented the wireless network composed of the aptasensor with a portable Wi-Fi fluorescent reader. Transmission success rate testing results show that an increase in node hops can promote the rate of transmission success dramatically. When the hop count is larger than 6, the rate of transmission success can reach more than 90% if the transmission failure probability and sleep probability are 0.1 and 0.5, respectively.

show abstract

Section: Introductionmentioning

confidence: 99%

Wireless Network of Silver Film Lysozyme Aptasensor Based on Fractal Measurement

Wang

2021

Security and Communication Networks

View full text Add to dashboard Cite

show abstract

“…The same group used a similar approach for the detection of lysozyme protein: in this case the electrode was functionalized with a DNA complementary to the lysozyme aptamer. In the presence of the enzyme, the aptamer is sequestered from the dsDNA complex, resulting in a higher blockade of the electrode ( Figure 14 B) [ 210 ]. In a relatable manner, molecular beacon structures were employed for the detection of target DNA through cyclic voltammetry, using dopamine as a reporter.…”

Section: 1 O 2 In Bioorganic Chemistry Ap...mentioning

confidence: 99%

A Photosensitized Singlet Oxygen (1O2) Toolbox for Bio-Organic Applications: Tailoring 1O2 Generation for DNA and Protein Labelling, Targeting and Biosensing

et al. 2022

View full text Add to dashboard Cite

Singlet oxygen (1O2) is the excited state of ground, triplet state, molecular oxygen (O2). Photosensitized 1O2 has been extensively studied as one of the reactive oxygen species (ROS), responsible for damage of cellular components (protein, DNA, lipids). On the other hand, its generation has been exploited in organic synthesis, as well as in photodynamic therapy for the treatment of various forms of cancer. The aim of this review is to highlight the versatility of 1O2, discussing the main bioorganic applications reported over the past decades, which rely on its production. After a brief introduction on the photosensitized production of 1O2, we will describe the main aspects involving the biologically relevant damage that can accompany an uncontrolled, aspecific generation of this ROS. We then discuss in more detail a series of biological applications featuring 1O2 generation, including protein and DNA labelling, cross-linking and biosensing. Finally, we will highlight the methodologies available to tailor 1O2 generation, in order to accomplish the proposed bioorganic transformations while avoiding, at the same time, collateral damage related to an untamed production of this reactive species.

show abstract

“…Therefore, we went on to determine the selectivity of the lysozyme sensor by characterizing its performance in the presence of various biomolecules individually and in a mixture. For this purpose, we selected previously reported interfering biomolecules: bovine serum albumin (BSA), glucose, and cytochrome C (Cyt-C) separately and in a mixture [24][25][26]30]. To make this study relevant to the biological context, concentrations of each of the interfering biomolecules were kept similar to their biological concentrations [54][55][56].…”

Section: Selectivity Of Lysozyme Aptasensormentioning

confidence: 99%

“…A number of techniques have been demonstrated for the detection of lysozyme including classical analytical methods such as chromatography [21] and enzyme-linked immunosorbent assay (ELISA) [22,23]. Recent techniques for lysozyme analysis include electrochemical [2,[24][25][26], optical [27][28][29], colorimetric [5,30,31], and surface plasmon resonance (SPR) [32,33], some of which are highly sensitive with detection limits in the picomolar to femtomolar ranges [1,2,24,26,27]. Nevertheless, most of these detection methods suffer from one or more problems such as low-selectivity, complex sample pre-treatment, time-consuming immobilizing processes, slow response time, etc.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

FRET-Based Aptasensor for the Selective and Sensitive Detection of Lysozyme

Sapkota¹,

Dhakal²

2020

Sensors

View full text Add to dashboard Cite

Lysozyme is a conserved antimicrobial enzyme and has been cited for its role in immune modulation. Increase in lysozyme concentration in body fluids is also regarded as an early warning of some diseases such as Alzheimer’s, sarcoidosis, Crohn’s disease, and breast cancer. Therefore, a method for a sensitive and selective detection of lysozyme can benefit many different areas of research. In this regard, several aptamers that are specific to lysozyme have been developed, but there is still a lack of a detection method that is sensitive, specific, and quantitative. In this work, we demonstrated a single-molecule fluorescence resonance energy transfer (smFRET)-based detection of lysozyme using an aptamer sensor (also called aptasensor) in which the binding of lysozyme triggers its conformational switch from a low-FRET to high-FRET state. Using this strategy, we demonstrated that the aptasensor is sensitive down to 2.3 picomoles (30 nM) of lysozyme with a dynamic range extending to ~2 µM and has little to no interference from similar biomolecules. The smFRET approach used here requires a dramatically small amount of aptasensor (~3000-fold less as compared to typical bulk fluorescence methods), and it is cost effective compared to enzymatic and antibody-based approaches. Additionally, the aptasensor can be readily regenerated in situ via a process called toehold mediated strand displacement (TMSD). The FRET-based aptasensing of lysozyme that we developed here could be implemented to detect other protein biomarkers by incorporating protein-specific aptamers without the need for changing fluorophore-labeled DNA strands.

show abstract

A facile electrochemical aptasensor for lysozyme detection based on target-induced turn-off of photosensitization

Cited by 28 publications

References 36 publications

Wireless Network of Silver Film Lysozyme Aptasensor Based on Fractal Measurement

Wireless Network of Silver Film Lysozyme Aptasensor Based on Fractal Measurement

A Photosensitized Singlet Oxygen (1O2) Toolbox for Bio-Organic Applications: Tailoring 1O2 Generation for DNA and Protein Labelling, Targeting and Biosensing

FRET-Based Aptasensor for the Selective and Sensitive Detection of Lysozyme

Contact Info

Product

Resources

About