2024
DOI: 10.1002/cbic.202400866
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A Facile Label‐Free Colorimetric Assay for DNase I Activity Based on the Peroxidase‐Like Activity of Functional Nucleic Acids

Yi Song,
Xu‐Xia Yan,
Shao‐Yu Sun
et al.

Abstract: DNase I plays a crucial role in fundamental biological processes, including gene expression, DNA repair, and apoptosis. Therefore, it is important to develop a simple and efficient method to detect DNase I activity. In this work, a colorimetric assay based on functional nucleic acid for sensitive DNase I detection was reported. The functional nucleic acids with G‐rich bases exhibit peroxidase‐like activity under acetic acid conditions, capable of catalyzing the oxidation of chromogenic substrate 3,3',5,5'‐tetr… Show more

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