A facile one-pot synthesis of acrylated hyaluronic acid

Sigen, A; Xu, Qian; McMichael, Peter; Gao, Yongsheng; Li, Xin; Wang, Xi; Greiser, Udo; Zhou, Daohong; Wang, Wenxin

doi:10.1039/c7cc08648b

Cited by 22 publications

(4 citation statements)

References 25 publications

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“…Hyaluronic acid methacryloyl (HAMA) synthesis refers to an established protocol. 30 In short, 2.0 g of hyaluronic acid was dissolved in reverse osmosis water (150 mL) and stirred for 24 h at room temperature to dissolve completely. Subsequently, 100 mL of N,Ndimethylformamide was added dropwise to hyaluronic acid solution to obtain a water/N,N-dimethylformamide ratio of 3/2 (v/v).…”

Section: Methodsmentioning

confidence: 99%

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A Silk Fibroin Methacryloyl-Modified Hydrogel Promoting Cell Adhesion for Customized 3D Cell-Laden Structures

Huang

Liu

et al. 2022

ACS Appl. Polym. Mater.

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Cell adhesion is the physical basis of tissue formation. Developing a kind of bioink with tunable mechanical properties and the promotion of cell adhesion is a major challenge for tissue engineering. In the present paper, a kind of hydrogel with good printable performance was developed, and cell adhesion was promoted in cell-laden DLP (Digital Light Processing) 3D bioprinting as a flexible solution for customized 3D structures. The hydrogel was prepared by modifying gelatin methacryloyl (GelMA)/hyaluronic acid methacryloyl (HAMA) with silk fibroin methacryloyl (SilMA). The three kinds of materials contain significant components of the extracellular matrix (ECM), such as glycosaminoglycan and proteins. Rheological results indicate that the multicomponent hydrogel precursors exhibit good photocuring behavior and are very suitable for DLP 3D bioprinting. The mechanical and biological properties of hydrogel can be tuned flexibly by SilMA content. Besides this, the SilMA/GelMA/HAMA hydrogel shows higher adhesion strength than the GelMA/HAMA hydrogel. The addition of SilMA into GelMA/HAMA significantly increased cell actin expression and promoted cell adhesion. Furthermore, the cell-laden 3D printed SilMA/GelMA/HAMA porous scaffold structure can remain stable after 7 days of in vitro culture. The survival rate of L929 fibroblasts can be up to 87%, exhibiting a good cell morphology and proliferation. All these results indicate that the SilMA/GelMA/HAMA hydrogel promoting cell adhesion will have broad implications for customized 3D structures in tissue engineering.

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Section: Methodsmentioning

confidence: 99%

“…Hyaluronic acid methacryloyl (HAMA) synthesis refers to an established protocol . In short, 2.0 g of hyaluronic acid was dissolved in reverse osmosis water (150 mL) and stirred for 24 h at room temperature to dissolve completely.…”

Section: Methodsmentioning

confidence: 99%

A Silk Fibroin Methacryloyl-Modified Hydrogel Promoting Cell Adhesion for Customized 3D Cell-Laden Structures

Huang

Liu

et al. 2022

ACS Appl. Polym. Mater.

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“…Initially, we prepared the non-thermoresponsive naturally derived materials. For that, CHTMA, GELMA, LAMMA, and HAMA were synthesized by coupling of chitosan (CHT), gelatin (GEL), laminarin (LAM), and hyaluronic acid (HA), respectively, with methacrylic acid, methacrylic anhydride, or glycidyl methacrylate on the basis of previous procedures. ,− CHTMA, GELMA, LAMMA, and HAMA were obtained as white solids with a degree of substitution (DS) of 33%, 52%, 18%, and 11% or 29%, respectively. The insertion of methacrylate moieties onto the polymer backbones was confirmed and quantified by 1 H NMR or UV–vis spectroscopy analysis (Figures S1–S5).…”

mentioning

confidence: 99%

“…We also evaluated the temperature-responsive performance of methacrylated gelatin (GELMA) in this system. These polymers were chosen as simple and widely known platforms with described cytocompatibility − (Figure ).…”

mentioning

confidence: 99%

Universal Strategy for Designing Shape Memory Hydrogels

Costa

Gomes

et al. 2022

ACS Materials Lett.

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Smart polymeric biomaterials have been the focus of many recent biomedical studies, especially those with adaptability to defects and potential to be implanted in the human body. Herein we report a versatile and straightforward method to convert non-thermoresponsive hydrogels into thermoresponsive systems with shape memory ability. As a proof of concept, a thermoresponsive polyurethane mesh was embedded within a methacrylated chitosan (CHTMA), gelatin (GELMA), laminarin (LAMMA) or hyaluronic acid (HAMA) hydrogel network, which afforded hydrogel composites with shape memory ability. With this system, we achieved good to excellent shape fixity ratios (50–90%) and excellent shape recovery ratios (∼100%, almost instantaneously) at body temperature (37 °C). Cytocompatibility tests demonstrated good viability either with cells on top or encapsulated during all shape memory processes. This straightforward approach opens a broad range of possibilities to convey shape memory properties to virtually any synthetic or natural-based hydrogel for several biological and nonbiological applications.

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Extracellular Matrix‐Mimetic Hydrogels for Treating Neural Tissue Injury: A Focus on Fibrin, Hyaluronic Acid, and Elastin‐Like Polypeptide Hydrogels

Gilbert

2021

Adv Healthcare Materials

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Neurological and functional recovery is limited following central nervous system injury and severe injury to the peripheral nervous system. Extracellular matrix (ECM)-mimetic hydrogels are of particular interest as regenerative scaffolds for the injured nervous system as they provide 3D bioactive interfaces that modulate cellular response to the injury environment and provide naturally degradable scaffolding for effective tissue remodeling. In this review, three unique ECM-mimetic hydrogels used in models of neural injury are reviewed: fibrin hydrogels, which rely on a naturally occurring enzymatic gelation, hyaluronic acid hydrogels, which require chemical modification prior to chemical crosslinking, and elastin-like polypeptide (ELP) hydrogels, which exhibit a temperature-sensitive gelation. The hydrogels are reviewed by summarizing their unique biological properties, their use as drug depots, and their combination with other biomaterials, such as electrospun fibers and nanoparticles. This review is the first to focus on these three ECM-mimetic hydrogels for their use in neural tissue engineering. Additionally, this is the first review to summarize the use of ELP hydrogels for nervous system applications. ECM-mimetic hydrogels have shown great promise in preclinical models of neural injury and future advancements in their design and use can likely lead to viable treatments for patients with neural injury.

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A facile one-pot synthesis of acrylated hyaluronic acid

Cited by 22 publications

References 25 publications

A Silk Fibroin Methacryloyl-Modified Hydrogel Promoting Cell Adhesion for Customized 3D Cell-Laden Structures

A Silk Fibroin Methacryloyl-Modified Hydrogel Promoting Cell Adhesion for Customized 3D Cell-Laden Structures

Universal Strategy for Designing Shape Memory Hydrogels

Extracellular Matrix‐Mimetic Hydrogels for Treating Neural Tissue Injury: A Focus on Fibrin, Hyaluronic Acid, and Elastin‐Like Polypeptide Hydrogels

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