A facile one-stone-two-birds strategy for fabricating multifunctional 3D nanofibrous scaffolds

Amarjargal, Altangerel; Goudarzi, Zahra Moazzami; Olga, Cegielska; Gradys, Arkadiusz; Kołbuk, Dorota; Kałaska, Bartłomiej; Ruszczyńska, Anna

doi:10.1039/d3bm00837a

Cited by 8 publications

(6 citation statements)

References 65 publications

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“…Additionally, the single-drug loaded nanofibers (EE/OCT) did not meet the expected standards for cell viability percentage, probably due to the release of a large amount of drug during the initial phase of release. 54,55 In fact, the results suggest that monolithic nanofibers have the potential to be cytotoxic. Combining two or three known compounds synergistically can help enhance the effectiveness of therapy while also minimizing the required concentration of individual drugs, which ultimately helps lower the risk of drug toxicity.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

On-Demand Sequential Release of Dual Drug from pH-Responsive Electrospun Janus Nanofiber Membranes toward Wound Healing and Infection Control

Amarjargal,

Cegielska,

Kolbuk

et al. 2023

ACS Appl. Mater. Interfaces

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Drugs against bacteria and abnormal cells, such as antibiotics and anticancer drugs, may save human lives. However, drug resistance is becoming more common in the clinical world. Nowadays, a synergistic action of multiple bioactive compounds and their combination with smart nanoplatforms has been considered an alternative therapeutic strategy to fight drug resistance in multidrugresistant cancers and microorganisms. The present study reports a onestep fabrication of innovative pH-responsive Janus nanofibers loaded with two active compounds, each in separate polymer compartments for synergistic combination therapy. By dissolving one of the compartments from the nanofibers, we could clearly demonstrate a highly yielded anisotropic Janus structure with two faces by scanning electron microscopy (SEM) analysis. To better understand the distinctive attributes of Janus nanofibers, several analytical methods, such as X-ray diffraction (XRD), FTIR spectroscopy, and contact angle goniometry, were utilized to examine and compare them to those of monolithic nanofibers. Furthermore, a drug release test was conducted in pH 7.4 and 6.0 media since the properties of Janus nanofibers correlate significantly with different environmental pH levels. This resulted in the on-demand sequential codelivery of octenidine (OCT) and curcumin (CUR) to the corresponding pH stimulus. Accordingly, the antibacterial properties of Janus fibers against Escherichia coli and Staphylococcus aureus, tested in a suspension test, were pH-dependent, i.e., greater in pH 6 due to the synergistic action of two active compounds, and Eudragit E100 (EE), and highly satisfactory. The biocompatibility of the Janus fibers was confirmed in selected tests.

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Section: ■ Results and Discussionmentioning

confidence: 99%

On-Demand Sequential Release of Dual Drug from pH-Responsive Electrospun Janus Nanofiber Membranes toward Wound Healing and Infection Control

Amarjargal,

Cegielska,

Kolbuk

et al. 2023

ACS Appl. Mater. Interfaces

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“…The MG63 cell line is a human osteoblastic line recommended by the International Organization for Standardization (ISO) for assessment of biocompatibility in 10993-5 Biological evaluation of medical devices, Part 5: Tests for in vitro cytotoxicity. Hence, viability was determined on sample extracts, as in a previous study [ 53 ]. Additionally, MG-63 cells are used in osteogenesis studies because they remain stable in their phenotype over several passages in cell culture.…”

Section: Resultsmentioning

confidence: 99%

Development of Poly(methyl methacrylate)/nano-hydroxyapatite (PMMA/nHA) Nanofibers for Tissue Engineering Regeneration Using an Electrospinning Technique

Zaszczyńska,

Kołbuk,

Gradys

et al. 2024

Polymers

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The study explores the in vitro biocompatibility and osteoconductivity of poly(methyl methacrylate)/nano-hydroxyapatite (PMMA/nHA) composite nanofibrous scaffolds for bone tissue engineering (BTE). Electrospun scaffolds, exhibiting both low and high fiber orientation, were investigated. The inclusion of hydroxyapatite nanoparticles enhances the osteoconductivity of the scaffolds while maintaining the ease of fabrication through electrospinning. SEM analysis confirms the high-quality morphology of the scaffolds, with successful incorporation of nHA evidenced by SEM-EDS and FTIR methods. DSC analysis indicates that nHA addition increases the PMMA glass transition temperature (Tg) and reduces stress relaxation during electrospinning. Furthermore, higher fiber orientation affects PMMA Tg and stress relaxation differently. Biological studies demonstrate the composite material’s non-toxicity, excellent osteoblast viability, attachment, spreading, and proliferation. Overall, PMMA/nHA composite scaffolds show promise for BTE applications.

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“…Various manufacturing techniques have been devised to produce porous architectures that have antibacterial properties, such as phase separation, supercritical fluid, hydrogel, and electrospinning (Figure 3) [75][76][77][78][79]. Although electrostatic spinning has become popular in the field of biomedical engineering in recent years, its history can be traced back to the 1600s, when English physicist William Gilbert first described the electrostatic phenomena [80].…”

Section: Electrospinning As a Methods For Wound Dressing Formationmentioning

confidence: 99%

“…Researchers address this issue by integrating antibacterial agents into materials intrinsically endowed with antibacterial properties. These materials encompass various polymers, including chitosan, collagen, alginate, and silver (Ag), copper (Cu), zinc oxide (ZnO), and gold (Au) nanoparticles ( Figure 5 ), as documented in Table 2 and Table 3 [ 76 , 85 , 92 , 96 , 116 , 117 , 118 ]. Dressings endowed with intrinsic antibacterial efficacy offer sustained benefits and reduced cytotoxicity compared to those releasing antibacterial agents, as shown in Figure 3 [ 84 , 85 ].…”

Section: Antibacterial Nanofibers For Wound Dressingmentioning

confidence: 99%

“…Recently, researchers have developed a biomimetic electrospun nanofibrous wound dressing that incorporates both chitosan and Ag NPs to address bacterial infection and promote tissue repair [ 89 ]. Altangerel et al [ 76 ] have developed a simple way to create nanofibrous scaffolds in 3D that have strong antibacterial properties, which are particularly useful in treating slowly healing diabetic wounds. Octenidine (OCT) is a cationic surfactant and antimicrobial agent that can transform a 2D membrane into a hydrophilic 3D scaffold in a “two birds, one stone” manner.…”

Section: Antibacterial Nanofibers For Wound Dressingmentioning

confidence: 99%

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Electrospun Antimicrobial Drug Delivery Systems and Hydrogels Used for Wound Dressings

Moazzami Goudarzi,

Zaszczyńska,

Kowalczyk

et al. 2024

Pharmaceutics

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Wounds and chronic wounds can be caused by bacterial infections and lead to discomfort in patients. To solve this problem, scientists are working to create modern wound dressings with antibacterial additives, mainly because traditional materials cannot meet the general requirements for complex wounds and cannot promote wound healing. This demand is met by material engineering, through which we can create electrospun wound dressings. Electrospun wound dressings, as well as those based on hydrogels with incorporated antibacterial compounds, can meet these requirements. This manuscript reviews recent materials used as wound dressings, discussing their formation, application, and functionalization. The focus is on presenting dressings based on electrospun materials and hydrogels. In contrast, recent advancements in wound care have highlighted the potential of thermoresponsive hydrogels as dynamic and antibacterial wound dressings. These hydrogels contain adaptable polymers that offer targeted drug delivery and show promise in managing various wound types while addressing bacterial infections. In this way, the article is intended to serve as a compendium of knowledge for researchers, medical practitioners, and biomaterials engineers, providing up-to-date information on the state of the art, possibilities of innovative solutions, and potential challenges in the area of materials used in dressings.

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A facile one-stone-two-birds strategy for fabricating multifunctional 3D nanofibrous scaffolds

Abstract: Local bacterial infections lead to delayed wound healing and, in extreme cases, such as diabetic foot ulcers, to non-healing due to the impaired cellular function in such wounds. Thus, many...

Cited by 8 publications

References 65 publications

On-Demand Sequential Release of Dual Drug from pH-Responsive Electrospun Janus Nanofiber Membranes toward Wound Healing and Infection Control

On-Demand Sequential Release of Dual Drug from pH-Responsive Electrospun Janus Nanofiber Membranes toward Wound Healing and Infection Control

Development of Poly(methyl methacrylate)/nano-hydroxyapatite (PMMA/nHA) Nanofibers for Tissue Engineering Regeneration Using an Electrospinning Technique

Electrospun Antimicrobial Drug Delivery Systems and Hydrogels Used for Wound Dressings

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