A facile preparation of highly interconnected macroporous PLGA scaffolds by liquid–liquid phase separation II

Shin, Kyu Chul; Kim, Bong Sup; Kim, Ji Heung; Park, Tae Gwan; Nam, Jae Do; Lee, Sang Soo

doi:10.1016/j.polymer.2005.02.114

Cited by 53 publications

(35 citation statements)

References 34 publications

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“…The phase separation technique investigated herein has been proven to be very flexible and useful to prepare scaffolds for bone tissue engineering applications. The polymeric gel obtained can be moulded in any shape and size (52)(53)(54)(55). Microporosity of the final object is dependent on the ratio between THF (good solvent for PCL) and water (bad solvent); instead macroporosity is obtained by adding an insoluble material (porogen) with a defined size to the gel phase.…”

Section: Discussionmentioning

confidence: 99%

“…Almost all techniques allow the production of porous, biodegradable scaffolds, which, however, result do not appear to be suitable for bone regeneration: fiber bonding and gas foaming techniques do not allow for a fine control of porosity, while solvent-casting/particulate leaching is used to produce only thin scaffolds (thickness ~3mm) which lack the mechanical property required for the load-bearing tissue. In contrast, the phase separation technique is characterized by high flexibility and could be appealing for obtaining bone tissue-engineered scaffolds: various parameters can be changed to tailor pore size and porosity and scaffolds with controlled morphology, defined shape and size (52)(53)(54)(55).…”

Section: Introductionmentioning

confidence: 99%

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Porous alginate/poly(ε-caprolactone) scaffolds: preparation, characterization and in vitro biological activity

Grandi

Liddo

Paganin³

et al. 2011

Int J Mol Med

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Abstract. In bone tissue engineering, scaffolds with controlled porosity are required to allow cell ingrowth, nutrient diffusion and sufficient formation of vascular networks. The physical properties of synthetic scaffolds are known to be dependent on the biomaterial type and its processing technique. In this study, we demonstrate that the separation phase technique is a useful method to process poly(Â-caprolactone) (PCL) into a desired shape and size. Moreover, using poly(ethylene glycol), sucrose, fructose and Ca 2+ alginate as porogen agents, we obtained PCL scaffolds with three-dimensional porous structures characterized by different pore size and geometry. Scanning electron microscopy and porosity analysis indicated that PCL scaffolds prepared with Ca 2+ alginate threads resemble the porosity and the homogeneous pore size distribution of native bone. In parallel, MicroCT analysis confirmed the presence of interconnected void spaces suitable to guarantee a biological environment for cellular growth, as demonstrated by a biocompatibility test with MC3T3-E1 murine preosteoblastic cells. In particular, scaffolds prepared with Ca 2+ alginate threads increased adhesion and proliferation of MC3T3-E1 cells under basal culture conditions, and upon stimulation with a specific differentiation culture medium they enhanced the early and later differentiated cell functions, including alkaline phosphatase activity and mineralized extracellular matrix production. These results suggest that PCL scaffolds, obtained by separation phase technique and prepared with alginate threads, could be considered as candidates for bone tissue engineering applications, possessing the required physical and biological properties.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Porous alginate/poly(ε-caprolactone) scaffolds: preparation, characterization and in vitro biological activity

Grandi

Liddo

Paganin³

et al. 2011

Int J Mol Med

View full text Add to dashboard Cite

show abstract

“…However, the effect of the molecular weight of the polymer seems to be less prominent (Hua et al, 2003). The phase behavior of the system is also be affected by adding various compounds (Hua et al, 2001;Shin et al, 2005). It has been demonstrated that by adding a surface active material such as Pluronic F127, a tri-block polymeric surfactant, the interfacial energy between two phases can be reduced, effectively stabilizing the porous structure of the scaffolds (Nam & Park, 1999).…”

Section: Polymer Scaffolds Prepared Via Tipsmentioning

confidence: 99%

Scaffolds for Tissue Engineering Via Thermally Induced Phase Separation

Martínez-Pérez¹,

Olivas–Armendáriz²,

Castro-Carmona³

et al. 2011

Advances in Regenerative Medicine

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“…It should be noted that for simplicity reasons, the moduli for the transversely isotropic case (E 3 and C 11 in Eqs. [7][8][9][10][11][12][13] are represented by E z and H A , respectively. Figure 5 shows a comparison between the fits obtained based on purely isotopic and transversely isotropic biphasic models.…”

Section: Biphasic Parameter Estimationmentioning

confidence: 99%

“…A variety of techniques is currently being employed for fabrication of porous polymeric scaffolds, including phase separation [10,11], gas foaming [12], porogen leaching [13], fiber bonding [14], emulsion freeze-drying [15], or a combination of these techniques [16][17][18]. While the conventional scaffold fabrication techniques can produce highly porous scaffolds, they have very limited control over scaffold architecture and pore interconnectivity.…”

Section: Introductionmentioning

confidence: 99%

Design and fabrication of 3D‐plotted polymeric scaffolds in functional tissue engineering

Yousefi¹,

Gauvin²,

Sun³

et al. 2007

Polymer Engineering & Sci

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Regenerating the load‐bearing tissues requires 3D scaffolds that balance the temporary mechanical function with the biological requirements. In functional tissue engineering, designing scaffolds with biomimetic mechanical properties could promote tissue ingrowth since the cells are sensitive to their local mechanical environment. This work aims to design scaffolds that mimic the mechanical response of the biological tissues under physiological loading conditions. Poly(L‐lactide) (PLLA) scaffolds with varying porosities and pore sizes were made by the 3D‐plotting technique. The scaffolds were tested under unconfined ramp compression to compare their stress profile under load with that of bovine cartilage. A comparison between the material parameters estimated for the scaffolds and for the bovine cartilage based on the biphasic theory enabled the definition of an optimum window for the porosity and pore size of these constructs. Moreover, the finite element prediction for the stress distribution inside the scaffolds, surrounded by the host cartilaginous tissue, demonstrated a negligible perturbation of the stress field at the site of implantation. The finite element modeling tools in combination with the developed methodology for optimal porosity/pore size determination can be used to improve the design of biomimetic scaffolds. POLYM. ENG. SCI., 47:608–618, 2007. © 2007 Society of Plastics Engineers.

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A facile preparation of highly interconnected macroporous PLGA scaffolds by liquid–liquid phase separation II

Cited by 53 publications

References 34 publications

Porous alginate/poly(ε-caprolactone) scaffolds: preparation, characterization and in vitro biological activity

Porous alginate/poly(ε-caprolactone) scaffolds: preparation, characterization and in vitro biological activity

Scaffolds for Tissue Engineering Via Thermally Induced Phase Separation

Design and fabrication of 3D‐plotted polymeric scaffolds in functional tissue engineering

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