A facile strategy for fabricating composite patch scaffold by using porcine acellular dermal matrix and gelatin for the reconstruction of abdominal wall defects

Abstract

Defects of the abdominal wall continue to be a significant issue in regenerative medicine and tissue engineering. Porcine acellular dermal matrix (P-ADM) and gelatin have been widely used for fabricating engineered abdominal wall due to their good biocompatibility and integration with host tissues. In this study, we demonstrate a novel and reproducible approach of using gelatin and P-ADM powder to fabricate composite patch scaffolds by combining solvent casting and enzymatic cross-linking for the abdominal wall regeneration in rats. Different concentration ratios of gelatin and P-ADM and different shapes of engineered scaffolds are studied for various abdominal wall defects. Skeletal muscle cells and human umbilical vein endothelial cells can successfully attach to the surface of the scaffolds and maintain high viability after culturing for five days. In vivo experiments demonstrate that there are no signs of infection, intra-abdominal adhesion, and severe inflammation after two weeks, which indicate good repairability of the scaffolds. In conclusion, we propose a novel method that holds great potential for building engineered scaffolds to meet the different requirements of abdominal wall regeneration.

A Review of Advanced Abdominal Wall Hernia Patch Materials

“…It is commonly used as a substitute for Col in healthcare and biomaterial applications. [154][155][156] Hydrolysis of Col's rod-like triple helical structure generates its separation into random and locally fragmented molecular structure, [150] causing Col to decompose into linear structures with molecular weights ranging from 15 to 250 kDa. [155] While the amino acid composition of GT bears some resemblance to that of Col, their architectures and qualities can differ considerably depending on the amino acid sequence and pretreatment.…”

Designing Double‐Layer Multimaterial Composite Patch Scaffold with Adhesion Resistance for Hernia Repair

“…Moreover, a composite hydrogel patch scaffold were developed by using gelatin and porcine acellular dermal matrix powder based on solvent casting and enzymatic cross-linking; although the scaffold shown excellent biocompatibility, lack of mechanical and sutural properties. [11] In order to solve the above situation, Pascual et al added oxidized collagen/chitosan (CS) coating on polypropylene (PP) patches, and this patch showed good anti-adhesive effect and tissue infiltration. [12] The coating of oxidized regenerated cellulose (ORC) on PP patches by Aydemir Sezer et al resulted in good antiadhesive effect and mechanical properties of the patches.…”

3D Printing GelMA/PVA Interpenetrating Polymer Networks Scaffolds Mediated with CuO Nanoparticles for Angiogenesis

“…In addition, angiogenesis is a key process for the repair and regeneration of damaged tissues, and angiogenesis of tissueengineering scaffold is also one of the great challenges for large-volume construction. [30] Recently, many strategies have been reported to solve this issue, such as construction of pre-vascularized channel within scaffolds [31][32][33][34] or using angiogenesis agents to spontaneously form endothelial cell vascular networks, [35][36][37][38] where angiogenesis agents exhibited greater potential in self-promote vascularization of tissue-engineering scaffold. [39] For many years, researchers have discovered that the angiogenic properties of conventional angiogenesis agents (e.g., growth factors) are primarily dependent on reactive oxygen species (ROS).…”