A facile strategy to functionalize gold nanorods with polycation brushes for biomedical applications

Peng, Yan; Zhao, Nana; Hu, Hao; Lin, Xinyi; Liu, Fusheng; Xu, Fu‐Jian

doi:10.1016/j.actbio.2014.05.002

Cited by 41 publications

(57 citation statements)

References 37 publications

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“…Synthesis of Au NRs : The synthetic details on the preparation of Au NRs through seed‐mediated approach were reported in our earlier work . In brief, Au seeds were prepared by adding the ice‐cold NaBH 4 solution to the mixture of CTAB and HAuCl 4 , which were placed in a water bath at 27 °C for 1 h before use.…”

Section: Methodsmentioning

confidence: 99%

“…Cytotoxicity Assay : The cytotoxicity of the nanohybrids was evaluated using MTT assay in HeLa and C6 cell lines following the procedure described in our previous work . The cells were cultured with 100 μL of fresh Dulbecco's modified eagle medium (DMEM) containing PGED/pDNA, PGEA/pDNA, Au‐PGED/pDNA, or Au‐PGEA/pDNA complexes at different N/P ratios and kept for 4 h. Then, the complexes were removed completely and the cells were cultured in fresh normal medium for additional 20 h. The final absorbance of the produced formazan in DMSO at a wavelength of 570 nm was measured using a Bio‐Rad Model 680 Microplate Reader (UK).…”

Section: Methodsmentioning

confidence: 99%

“…It was demonstrated that profound gene carriers could be fabricated by linking cationic polymers with inorganic NPs to take advantage of both . Particularly, a strategy was developed to functionalize Au NRs with poly(2‐( N,N ‐dimethyl amino)ethyl methacrylate) (PDMAEMA) via “grafting‐from” approach . Although the as‐prepared Au NRs exhibited great potential for GT and computed tomography (CT) imaging in vitro, the well‐known high cytotoxicity of PDMAEMA limits them for future clinical applications.…”

Section: Introductionmentioning

confidence: 99%

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Hierarchical Nanohybrids of Gold Nanorods and PGMA‐Based Polycations for Multifunctional Theranostics

Zhao

Zhou

et al. 2016

Adv Funct Materials

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Organic/inorganic nanohybrids hold great importance in fabricating multifunctional theranostics to integrate therapeutic functions with real-time imaging. Although Au nanorods (NRs) have been employed for theranostics, complicated design of materials limits their practical applications. In this work, new multifunctional theranostic agents are designed and synthesized employing Au NRs with desirable near-infrared absorbance as the cores. A facile "grafting-onto" approach is put forward to prepare the series of hierarchical nanohybrids (Au-PGEA and Au-PGED) of Au NRs and poly(glycidyl methacrylate)-based polycations. The resultant nanohybrids can be utilized as gene carriers with high gene transfection performances. The structural effect of polycations on gene transfection is investigated in detail, and Au-PGEA with abundant hydroxyl groups on the surface exhibits superior performance. Au-PGEA nanohybrids are further validated to possess remarkable capability of combined photothermal therapy (PTT) and gene therapy (GT) for complementary tumor treatment. Moreover, signifi cantly enhanced computed tomography (CT)/photoacoustic (PA) signals are detected both in vitro and in vivo, verifying the potential of Au-PGEA for dual-modal imaging with precise and accurate information. Therefore, these multifunctional nanohybrids fabricated from a simple and straightforward strategy are promising for in vivo dualmodal CT/PA imaging guided GT/PTT therapy with high antitumor effi cacy.advantage of both. [ 20,21 ] Particularly, a strategy was developed to functionalize Au NRs with poly(2-( N,N -dimethyl amino)ethyl methacrylate) (PDMAEMA) via "grafting-from" approach. [ 22,23 ] Although the as-prepared Au NRs exhibited great potential for GT and computed tomography (CT) imaging in vitro, the wellknown high cytotoxicity of PDMAEMA limits them for future clinical applications. Our recent study illustrates that compared with PDMAEMA, ethanolamine (EA) or ethylenediamine (ED)functionalized poly(glycidyl methacrylate) (PGMA) (denoted by BUCT-PGEA or PGED) exhibit lower cytotoxicity and superior gene transfection performances. [24][25][26] Besides the identical cationic pedant secondary amine and hydroxyl groups of PGEA, PGED also contains fl anking primary amine groups, [ 25 ] which allows tailoring the surface property of Au NRs through structural effects. These results inspire us to design Au NRs functionalized with PGMA-based polycations for theranostics.Herein, we exploit a facile preparation of organic/inorganic hierarchical nanohybrids based on PGEA-and PGED-modifi ed Au NRs for dual-modal imaging-guided combination of PTT/GT cancer treatment. It is noted that if the "grafting-from" strategy was applied, Au NRs would endure clustering and aggregation during preparation, resulting in loss of the NIR absorption property. In order to overcome this obstacle, the "grafting-onto" approach was put forward for the surface functionalization. In this regard, lipoic acid (LA) or lipoic acid anhydride (LA-LA) was successfully linked with PGEA...

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hierarchical Nanohybrids of Gold Nanorods and PGMA‐Based Polycations for Multifunctional Theranostics

Zhao

Zhou

et al. 2016

Adv Funct Materials

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“…Therefore, functionalization enhanced the image contrast by particular binding to Hydroxyapatite crystals. Gold nanorods can be used as CT imaging agents as well as gene carriers through functionalization of interested gene through poly(2‐( N , N ‐dimethyl amino)ethyl methacrylate) 64. Lalwani et al incorporated manganese ions within the graphene sheets and functionalized with iodine in equimolar concentration to create dual probes, enabling MR imaging and PET 65.…”

Section: Functionalization To Enhance Imagingmentioning

confidence: 99%

Nanoparticle Functionalization and Its Potentials for Molecular Imaging

et al. 2016

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Functionalization enhances the properties and characteristics of nanoparticles through surface modification, and enables them to play a major role in the field of medicine. In molecular imaging, quality functional images are required with proper differentiation which can be seen with high contrast to obtain viable information. This review article discusses how functionalization enhances molecular imaging and enables multimodal imaging by which images with combination of functions particular to each modality can be obtained. This also explains how nanoparticles interacting at molecular level, when functionalized with molecules can target the cells of interest or substances with high specificity, reducing background signal and allowing simultaneous therapies to be carried out while imaging. Functionalization allows imaging for a prolonged period and enables to track the cells over a period of time. Recent researches and progress in functionalizing the nanoparticles to specifically enhance bioimaging with different modalities and their applications are reviewed in this article.

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“…Through appropriate surface functionalization, the hetero‐nanoparticles can be employed to construct organic/inorganic nanohybrids with more integrated functions (e.g., gene delivery, imaging, and chemotherapy) . Nanohybrids taking advantages of both organic and inorganic components were synthesized to realize combined functions of gene therapy and imaging, while the cytotoxicity is still not satisfying . Recently, a series of low‐toxic hydroxyl‐rich polycations based on ethanolamine (EA)‐modified poly(glycidyl methacrylate) (PGMA), namely BUCT‐PGEA, have been demonstrate as a kind of excellent gene carriers with low cytotoxicity .…”

Section: Introductionmentioning

confidence: 99%

NIR‐Responsive Polycationic Gatekeeper‐Cloaked Hetero‐Nanoparticles for Multimodal Imaging‐Guided Triple‐Combination Therapy of Cancer

Duan

Yang

Zhang

et al. 2016

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Responsive multifunctional organic/inorganic nanohybrids are promising for effective and precise imaging-guided therapy of cancer. In this work, a near-infrared (NIR)-triggered multifunctional nanoplatform comprising Au nanorods (Au NRs), mesoporous silica, quantum dots (QDs), and two-armed ethanolamine-modified poly(glycidyl methacrylate) with cyclodextrin cores (denoted as CD-PGEA) has been successfully fabricated for multimodal imaging-guided triple-combination treatment of cancer. A hierarchical hetero-structure is first constructed via integration of Au NRs with QDs through a mesoporous silica intermediate layer. The X-ray opacity and photoacoustic (PA) property of Au NRs are utilized for tomography (CT) and PA imaging, and the imaging sensitivity is further enhanced by the fluorescent QDs. The mesoporous feature of silica allows the loading of a typical antitumor drug, doxorubicin (DOX), which are sealed by the polycationic gatekeepers, low toxic hydroxyl-rich CD-PGEA/pDNA complexes, realizing the co-delivery of drug and gene. The photothermal effect of Au NRs is utilized for photothermal therapy (PTT). More interestingly, such photothermal effect also induces a cascade of NIR-triggered release of DOX through the facilitated detachment of CD-PGEA gatekeepers for controlled chemotherapy. The resultant chemotherapy and gene therapy for glioma tumors are complementary for the efficiency of PTT. This work presents a novel responsive multifunctional imaging-guided therapy platform, which combines fluorescent/PA/CT imaging and gene/chemo/photothermal therapy into one nanostructure.

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A facile strategy to functionalize gold nanorods with polycation brushes for biomedical applications

Cited by 41 publications

References 37 publications

Hierarchical Nanohybrids of Gold Nanorods and PGMA‐Based Polycations for Multifunctional Theranostics

Hierarchical Nanohybrids of Gold Nanorods and PGMA‐Based Polycations for Multifunctional Theranostics

Nanoparticle Functionalization and Its Potentials for Molecular Imaging

NIR‐Responsive Polycationic Gatekeeper‐Cloaked Hetero‐Nanoparticles for Multimodal Imaging‐Guided Triple‐Combination Therapy of Cancer

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