A Facile Synthesis of a DifluoromethyleneAnalog of β-Aspartyl Phosphate as an Inhibitor ofl-Aspartate-β-semialdehyde Dehydrogenase

Abstract

An efficient and high yield procedure for the synthesis of the difluoromethylene analog of β-aspartyl phosphate, β-aspartyl difluoromethylenephosphonate (βAFP), is described. Preincubation of βAFP with L-aspartate-β-semialdehyde dehydrogenases from several infectious microorganisms leads to potent time-dependent enzyme inactivation.