The green synthesis of metal oxide nanoparticles is treated to be an eco-friendly path and cost-effectiveness. Mulberry plant extract was used to synthesize CuO nanoparticles in this study. UV spectroscopy absorbance at 370 nm was used to analyze the resulting synthesized nanoparticles. The distribution of nanoparticles and particle sizes were discovered to be in the range of 40-60 nm using scanning electron microscopy. Copper nanoparticles have characteristic diffraction peaks in the X-ray diffraction spectrum at 38.81°, and 66.35° which correspond to lattice planes (111) and (202), respectively. The antioxidant and antibacterial activity of the CuO nanoparticles was tested against gram-positive and gram-negative microorganisms. The results support the hypothesis that green synthesized CuO NPs could be a viable treatment option for diseases caused by microbial pathogens. This fact-finding result is that Mulberry plant extract-based green synthesized CuO nanoparticles destroyed or considerably inhibited pathogen activity, as well as having high antioxidant activity.