A family of cationic polyamides for in vitro and in vivo gene transfection

Zhang, Chengnan; Jin, Rong; Zhao, Peng; Lin, Chao

doi:10.1016/j.actbio.2015.04.025

Cited by 21 publications

(13 citation statements)

References 29 publications

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“…With the development of the chemistry of high-molecular compounds, polymeric materials have been widely used for implants: polyethylene, polyamides, polylactide, and polyglycolide, which, in addition to biological inertness, possess high mechanical properties and strength characteristics. For example, polyethylene contains residual free radicals, leading to oxidative degradation of the product 3 ; polyamides undergo shrinkage and are prone to deformation and rapid loss of mechanical strength 4 ; polylactide has technological difficulties in production and poor wettability 5 ; polyglycolide has high rigidity and low elasticity 6 . In addition, in the hydrolysis of polylactide and polyglycolide, acids are formed, which can lead to inflammation of the surrounding tissues and subsequent rejection of the material 7 .…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Magnesium- and Silicon-modified Hydroxyapatites by Microwave-Assisted Method

Rasskazova

Zhuk

Коротченко

et al. 2019

Sci Rep

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Nanopowders of hydroxyapatite (HA), modified by magnesium (MgHA) and by silicon (SiHA) were obtained by liquid-phase microwave synthesis method. X-ray diffraction and IR spectroscopy results showed that Mg2+ and SiO44− ions were present in the synthesized products both as secondary phases and as part of the HA phase. Whitlockite was found in the magnesium-modified HA (MgHA) and larnite was found in the silicon-modified HA (SiHA); ion substitution for both materials resulted in solid solutions. In the synthesized samples of modified HA, the increase of particle size of powders was in the order HA < SiHA < MgHA, which was calculated through data specific surface area and measured pycnometric density of the powders. The Lewis acid sites (Ca2+, Mg2+, Si4+) were present using spectral probes on the surface of the samples of HA, MgHA, and SiHA, and the acidity of these sites decreased in the order SiHA > MgHA > HA. The rates of calcium phosphate layer deposition on the surface of these materials at 37 °C in the model simulated body fluid solution showed similar dependence.

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Section: Introductionmentioning

confidence: 99%

Synthesis of Magnesium- and Silicon-modified Hydroxyapatites by Microwave-Assisted Method

Rasskazova

Zhuk

Коротченко

et al. 2019

Sci Rep

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“…В качестве альтернативных носителей в настоящее время все чаще используют синтетические поликатионные соединения, липиды и пептиды. Хотя их получение дешевле, чем вирусных векторов, недостатком является низкая эффективность и в ряде случаев высокая токсичность [6][7][8][9]. Наиболее безопасным может оказаться использование природных поликатионных белков, а также так называемых проникающих в клетки пептидов (CPP -cell penetration peptide).…”

Section: Introductionunclassified

Recombinant histones as an instrument for delivery of nucleic acids into eukaryotic cells

Zinovyeva¹,

Sass²,

Vvedensky³

et al. 2018

Mol. genet. mikrobiol. virusol. (rus.)

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Использование природных положительно заряженных белков в качестве носителя для транспорта нуклеиновых кислот может быть весьма перспективным для генной терапии. Наиболее привлекательными белками для этой цели являются гистоны. В работе описаны получение и очистка рекомбинантных гистонов H2A и H2B человека и химерного белка гистона Н2А с фрагментом белка ТАТ вируса HIV-1 (TAT-пептидом). Предлагаемый способ очистки гистоновых белков позволяет значительно снизить содержание бактериальных эндотоксинов в целевом препарате, что делает возможным использовать эти белки в экспериментах in vivo. Показана трансфицирующая активность коровых гистонов Н2А и Н2В и химерного гистона в комплексе с плазмидной ДНК. Обнаружена высокая специфичность эффективности трансфекции гистонов Н2А и Н2В для клеток линии НТ1080, при этом показано, что трансфекция комплексов плазмиды с химерным белком Н2А-ТАТ наблюдалась для многих линий клеток. Ключевые слова: невирусная доставка нуклеиновых кислот в клетки, очистка рекомбинантных гистонов, ТАТ-пептид, эндотоксин.

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“…This family of main-chain cationic polymers is named as poly(β-hydroxyl amine)s (PHA), which has a weakly basic characteristic on account of its tertiary amines with a p K b value of about 6.5. Self-assembly of the biodegradable poly(β-hydroxyl amine)s has been studied in recent years, but due to poor hydrophilicity provided by hydroxyl groups and tertiary amines, the main-chain cationic polymers always were linked by hydrophilic segments or polymers to build amphiphilic copolymers and corresponding stimuli-responsive copolymeric micelles in water for intracellular doxorubicin delivery …”

Section: Introductionmentioning

confidence: 99%

Facile Construction of pH- and Redox-Responsive Micelles from a Biodegradable Poly(β-hydroxyl amine) for Drug Delivery

Zhang

et al. 2015

Biomacromolecules

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Here we demonstrate a type of pH and reduction dual-sensitive biodegradable micelles, which were self-assembled by a cationic polymer in an aqueous solution. Due to tumor cells or tissues showing low pH and high reduction concentration, these micelles possessed specific tumor targetability and maximal drug-release controllability inside tumor cells upon changes in physical and chemical environments, but presented good stability at physiological conditions. CCK-8 assay showed that the DOX-loaded micelles had a similar cytotoxicity for MCF-7 tumor cells as free DOX, and blank micelles had a very low cytotoxicity to the cells. Fluorescent microscopy observation revealed that the drug-loaded micelles could be quickly internalized by endosomes to inhibit cancer cell growth. These results indicated these biodegradable micelles, as a novel and effective pH- and redox-responsive nanocarrier, have a potential to improve drug delivery and enhance the antitumor efficacy.

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A family of cationic polyamides for in vitro and in vivo gene transfection

Cited by 21 publications

References 29 publications

Synthesis of Magnesium- and Silicon-modified Hydroxyapatites by Microwave-Assisted Method

Synthesis of Magnesium- and Silicon-modified Hydroxyapatites by Microwave-Assisted Method

Recombinant histones as an instrument for delivery of nucleic acids into eukaryotic cells

Facile Construction of pH- and Redox-Responsive Micelles from a Biodegradable Poly(β-hydroxyl amine) for Drug Delivery

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