A fast and convenient new technique to detect the therapeutic target, K-ras mutant, from peripheral blood in non-small cell lung cancer patients

A prospective study comparing multi-gene biomarker chip and serum carcinoembryonic antigen in the postoperative surveillance for patients with stage I–III colorectal cancer

“…In the present study, the specificity (92.0%) and accuracy (91.3%), but not the sensitivity (87.5%), of the biomarker chip were similar to those reported in previous studies that used the WEnCA platform [18][19][20][21][22]. The false-positive rate of the biomarker chip in early prediction of postoperative relapse was 32.3%; nevertheless, postoperative serum CEA levels showed a higher false-positive rate (59.2%).…”

“…04 003 301.1). In order to implement the clinical application of specific gene groups, the oligonucleotide sequences of top 19 highly overexpressed target genes of the 71 genes were selected (Table 2), as described previously [18][19][20]; they were designed using the Oligo Explorer software program (Gene Link, Inc. New York, USA).…”

“…Each overexpressed spot was then multiplied by the respective weighted values ranging from 1 to 4, according to the principle described previously [18][19][20][21][22].…”

Clinical Application of Automatic Gene Chip Analyzer (WEnCA-Chipball) for Mutant Kras Detection in Peripheral Circulating Cancer Cells of Cancer Patients

“…After rapid drying and cross-linking procedures, the preparation of membrane array for target genes expression was accomplished. Our previous study developed and evaluated a membrane array-based method simultaneously detecting the expression levels of multiple mRNA markers from circulating cancer cells in peripheral blood for cancer diagnosis Yen et al, 2009;Tsao et al, 2010). We have carried out membrane array analysis using normal human adrenal cortical cells with KRAS mutation, and obtained 22 upregulated genes most closely related to the KRAS oncogene through bioinformatic analysis.…”

“…Since Northern blot involves complex steps and a large numbers of samples, its application is limited to research instead of clinical diagnosis. On the other hand, since RT-PCR and real-time PCR are performed through a series of simple steps, they are applied extensively for the detection of a single gene, as with the hepatitis virus and infectious pathogens Tsao et al, 2010). Although the invention of PCR ranks as one of the greatest discoveries of all time, most PCR techniques have a few common problems: (1) contamination, i.e., false positive results from oversensitive detection of, say, aerosolized DNA or previous sample carry-over; (2) RT-PCR is regarded as only semi-quantitative, since it is difficult to control the efficiency of sequence amplification when comparing different samples; and (3) interference is caused by annealing between the primers.…”