A Fast and Reliable Process to Fabricate Regenerated Silk Fibroin Solution from Degummed Silk in 4 Hours

Wöltje, Michael; Kölbel, Arthur; Aibibu, Dilbar; Cherif, Chokri

doi:10.3390/ijms221910565

Cited by 29 publications

(29 citation statements)

References 43 publications

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“…The separation of fibroin by solubilizing the sericin in water is termed as degumming. Degumming on a laboratory scale can be accomplished using a wide variety of techniques, including the application of highly concentrated urea, proteases, bromelain, biosurfactants, and organic acids such as boric acid sodium borate buffer, or citric acid, as well as infrared heating and microwave irradiation [ 60 , 61 , 62 , 63 ]. The most accepted technique for degumming involves boiling the silk thread in a buffer containing 0.02 M sodium carbonate (Na 2 CO 3 ) for 0.5 to 1 h, followed by repetitive washing to remove sericin completely.…”

Section: Silkmentioning

confidence: 99%

Silk-Based Biomaterials for Designing Bioinspired Microarchitecture for Various Biomedical Applications

et al. 2023

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Biomaterial research has led to revolutionary healthcare advances. Natural biological macromolecules can impact high-performance, multipurpose materials. This has prompted the quest for affordable healthcare solutions, with a focus on renewable biomaterials with a wide variety of applications and ecologically friendly techniques. Imitating their chemical compositions and hierarchical structures, bioinspired based materials have elevated rapidly over the past few decades. Bio-inspired strategies entail extracting fundamental components and reassembling them into programmable biomaterials. This method may improve its processability and modifiability, allowing it to meet the biological application criteria. Silk is a desirable biosourced raw material due to its high mechanical properties, flexibility, bioactive component sequestration, controlled biodegradability, remarkable biocompatibility, and inexpensiveness. Silk regulates temporo-spatial, biochemical and biophysical reactions. Extracellular biophysical factors regulate cellular destiny dynamically. This review examines the bioinspired structural and functional properties of silk material based scaffolds. We explored silk types, chemical composition, architecture, mechanical properties, topography, and 3D geometry to unlock the body’s innate regenerative potential, keeping in mind the novel biophysical properties of silk in film, fiber, and other potential forms, coupled with facile chemical changes, and its ability to match functional requirements for specific tissues.

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Section: Silkmentioning

confidence: 99%

Silk-Based Biomaterials for Designing Bioinspired Microarchitecture for Various Biomedical Applications

et al. 2023

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“…Unfortunately, these solvents are hazardous and tend to be environmentally unfriendly [116]. Furthermore, they can directly affect the characteristics of SF and particularly impact the mechanical properties of the resulting silk biomaterials in subsequent processing [117,118]. The classical regeneration of SF largely includes the use of toxic organic solvents, including calcium chloride/formic acid [106][107][108][109], hexafluoroisopropanol (HFIP) [110][111][112], lithium salt solutions such as lithium thiocyanate (LiSCN) [113] and lithium bromide (9.3 M LiBr-H 2 O) [114] and calcium nitrate/methanol (Ca(NO 3 ) 2 )/CH 3 OH) mixtures [115].…”

Section: Sf As a Green Materialsmentioning

confidence: 99%

Section: Sf As a Green Materialsmentioning

confidence: 99%

The Contribution of Silk Fibroin in Biomedical Engineering

Lujerdean

Baci

Cucu

et al. 2022

Insects

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Silk fibroin (SF) is a natural protein (biopolymer) extracted from the cocoons of Bombyx mori L. (silkworm). It has many properties of interest in the field of biotechnology, the most important being biodegradability, biocompatibility and robust mechanical strength with high tensile strength. SF is usually dissolved in water-based solvents and can be easily reconstructed into a variety of material formats, including films, mats, hydrogels, and sponges, by various fabrication techniques (spin coating, electrospinning, freeze-drying, and physical or chemical crosslinking). Furthermore, SF is a feasible material used in many biomedical applications, including tissue engineering (3D scaffolds, wounds dressing), cancer therapy (mimicking the tumor microenvironment), controlled drug delivery (SF-based complexes), and bone, eye and skin regeneration. In this review, we describe the structure, composition, general properties, and structure–properties relationship of SF. In addition, the main methods used for ecological extraction and processing of SF that make it a green material are discussed. Lastly, technological advances in the use of SF-based materials are addressed, especially in healthcare applications such as tissue engineering and cancer therapeutics.

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“…With different extraction protocols, the chemical structure of sericin and its amino acid composition changes, which could impact the potential biomedical application 54–56 . The degumming process is also fundamental for the extraction and regeneration of silk fibroin into an aqueous solution and for the structural integrity of the three subunits of the silk fibroin protein complex 57 . Thus, it is often a crucial first step for developing silk‐based biomaterials.…”

Section: Introductionmentioning

confidence: 99%

“… 54 , 55 , 56 The degumming process is also fundamental for the extraction and regeneration of silk fibroin into an aqueous solution and for the structural integrity of the three subunits of the silk fibroin protein complex. 57 Thus, it is often a crucial first step for developing silk‐based biomaterials.…”

Section: Introductionmentioning

confidence: 99%

Biomedical applications of silk and its role for intervertebral disc repair

et al. 2022

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Intervertebral disc (IVD) degeneration (IDD) is the main contributor to chronic low back pain. To date, the present therapies mainly focus on treating the symptoms caused by IDD rather than addressing the problem itself. For this reason, researchers have searched for a suitable biomaterial to repair and/or regenerate the IVD. A promising candidate to fill this gap is silk, which has already been used as a biomaterial for many years. Therefore, this review aims first to elaborate on the different origins from which silk is harvested, the individual composition, and the characteristics of each silk type. Another goal is to enlighten why silk is so suitable as a biomaterial, discuss its functionalization, and how it could be used for tissue engineering purposes.The second part of this review aims to provide an overview of preclinical studies using silk-based biomaterials to repair the inner region of the IVD, the nucleus pulposus (NP), and the IVD's outer area, the annulus fibrosus (AF). Since the NP and the AF differ fundamentally in their structure, different therapeutic approaches are required.Consequently, silk-containing hydrogels have been used mainly to repair the NP, and silk-based scaffolds have been used for the AF.

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A Fast and Reliable Process to Fabricate Regenerated Silk Fibroin Solution from Degummed Silk in 4 Hours

Cited by 29 publications

References 43 publications

Silk-Based Biomaterials for Designing Bioinspired Microarchitecture for Various Biomedical Applications

Silk-Based Biomaterials for Designing Bioinspired Microarchitecture for Various Biomedical Applications

The Contribution of Silk Fibroin in Biomedical Engineering

Biomedical applications of silk and its role for intervertebral disc repair

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