A fast and sensitive analytical procedure for monitoring of synthetic pyrethroid pesticides' metabolites in environmental water samples

Pasupuleti, Raghavendra Rao; Tsai, Pei‐Chien; Ponnusamy, Vinoth Kumar

doi:10.1016/j.microc.2019.05.030

Cited by 19 publications

(11 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The extraction (vortex) time is a critical factor in the VA‐SI‐LLME procedure as it influences the mass transfer of target analytes and thus affects the extraction recoveries . A vortex mixer was utilized for the extraction process as its vigorous agitation helps to increase the extraction recoveries of OPP metabolites.…”

Section: Resultsmentioning

confidence: 99%

“…The extraction (vortex) time is a critical factor in the VA-SI-LLME procedure as it influences the mass transfer of target analytes and thus affects the extraction recoveries. 31,32 A vortex mixer was utilized for the extraction process as its vigorous agitation helps to increase the extraction recoveries of OPP metabolites. The influence of the vortex agitation time on the extraction recoveries of OPP metabolites from urine samples was investigated by varying the time at 1, 2, 3, 4, and 5 min.…”

Section: Extraction (Vortex) Timementioning

confidence: 99%

See 1 more Smart Citation

Rapid and sensitive analytical procedure for biomonitoring of organophosphate pesticide metabolites in human urine samples using a vortex‐assisted salt‐induced liquid–liquid microextraction technique coupled with ultra‐high‐performance liquid chromatography/tandem mass spectrometry

Pasupuleti

Tsai

Lin

et al. 2020

Rapid Comm Mass Spectrometry

Self Cite

View full text Add to dashboard Cite

Rationale Organophosphate pesticides (OPPs) are the most commonly used insecticides around the world in various agricultural and domestic practices, and humans are frequently exposed to these hazardous insecticides that can lead to several chronic health effects. Therefore, a fast and sensitive analytical method is required for biomonitoring the markers of OPPs in humans for exposure estimation. In this study, a fast and sensitive analytical procedure was developed for the determination of the metabolites of OPPs in human urine samples. Methods Metabolites of OPPs were extracted from 2 mL of urine sample using a novel vortex‐assisted salt‐induced liquid–liquid microextraction (VA‐SI‐LLME) technique, and the preconcentrated metabolites were analyzed by ultra‐high‐performance liquid chromatography/tandem mass spectrometry (UHPLC/MS/MS). Various factors affecting the efficiency of VA‐SI‐LLME were thoroughly investigated. Results The metabolites of OPPs exhibited very good linearity over the concentration range between 0.05 and 50 ng mL−1 with coefficient (r2) values ranging between 0.9986 and 0.9999. The method showed excellent sensitivity with detection limits ranging from 0.01 to 0.03 ng mL−1 and quantification limits from 0.03 to 0.05 ng mL−1. The developed method was applied to the analysis of real samples and the recoveries ranged between 85.0 and 114.1% with related standard deviations <5%. Conclusions The results showed the VA‐SI‐LLME/UHPLC/MS/MS method to be a simple, rapid, sensitive, and selective analytical procedure for the biomonitoring of the metabolites of OPPs in humans. This efficient and cost‐effective analytical method could be a potential alternative method for the biomonitoring of the metabolites of pesticides in humans.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Extraction (Vortex) Timementioning

confidence: 99%

Rapid and sensitive analytical procedure for biomonitoring of organophosphate pesticide metabolites in human urine samples using a vortex‐assisted salt‐induced liquid–liquid microextraction technique coupled with ultra‐high‐performance liquid chromatography/tandem mass spectrometry

Pasupuleti

Tsai

Lin

et al. 2020

Rapid Comm Mass Spectrometry

Self Cite

View full text Add to dashboard Cite

show abstract

“…Several techniques have been developed for the determination of pyrethroid transformation products and metabolites using gas chromatography tandem mass (GC-MS/MS) [ 15 ], ultra-high performance liquid chromatography (UHPLC) [ 16 ], and UHPLC coupled with tandem mass spectrometry (UHPLC-MS/MS) [ 17 ] or high-resolution orbitrap mass spectrometry (UHPLC-Orbitrap MS) [ 18 ]. Due to the high polarity and low volatility of pyrethroids transformation products and metabolites, these compounds were derived with bis(trimethylsilyl) trifluoroacetamide (BSTFA) prior to GC-MS/MS analysis [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

“…The UHPLC-MS/MS technique provided clear advantages for the analysis of transformation products and metabolites attributing to high selectivity, sensitivity, and accuracy. Although several approaches for the analysis of pyrethroid metabolites have been developed using UHPLC-MS/MS, these analytical protocols focused on environment water [ 17 ], soil [ 20 ], human urine, and plasma [ 21 , 22 ]. There are few methods for the determination of pyrethroid metabolites in agricultural crops and foods.…”

Section: Introductionmentioning

confidence: 99%

“…There are few methods for the determination of pyrethroid metabolites in agricultural crops and foods. For some simple matrices, such as human plasma and water [ 17 ], sample preparation did not involve clean-up [ 22 ]. However, for complex samples, such as soil [ 20 ], urine [ 21 ], wastewater [ 23 ], vegetables and fruits [ 24 ], and other substrates, it is necessary to carefully optimize the adsorbents and their dosage of dispersive solid-phase extraction (d-SPE) or SPE cartridges to obtain satisfactory purification effect.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Determination of Three Typical Metabolites of Pyrethroid Pesticides in Tea Using a Modified QuEChERS Sample Preparation by Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry

Chen

Wang

Liu

et al. 2021

Foods

View full text Add to dashboard Cite

Pyrethroid pesticides are widely used on tea plants, and their residues of high frequency and concentration have received great attention. Until recently, the residues of typical metabolites of pyrethroid pesticides in tea were unknown. Herein, a modified “quick, easy, cheap, effective, rugged and safe” (QuEChERS) method for the determination of three typical metabolites of pyrethroid pesticides in tea, using ultra performance liquid chromatography tandem mass spectrometry, was developed. The mixture of florisil, octadecylsilane, and graphite carbon black was employed as modified QuEChERS adsorbents. A Kinetex C18 column achieved good separation and chromatographic peaks of all analytes. The calibration curves of 3-phenoxybenzoic acid (3-PBA) and 4-fluoro-3-phenoxybenzoic acid (4-F-3-PBA) were linear in the range of 0.1–50 ng mL−1 (determination coefficient R2 higher than 0.999), and that of cis-3-(2-chloro-3,3,3-trifluoroprop-1-en-1-yl)-2,2-dimethylcyclopropanecarboxylic acid (TFA) was in the range of 1–100 ng mL−1 (R2 higher than 0.998). The method was validated and recoveries ranged from 83.0% to 117.3%. Intra- and inter-day precisions were lower than or equal to 13.2%. The limits of quantification of 3-PBA, 4-F-3-PBA, and TFA were 5, 2, and 10 μg kg−1, respectively. A total of 22 tea samples were monitored using this method, and 3-PBA and TFA were found in two green tea samples.

show abstract

Homogeneous liquid–liquid extraction as an alternative sample preparation technique for biomedical analysis

Hammad

Abdallah

Bedair

et al. 2021

J of Separation Science

View full text Add to dashboard Cite

Liquid-liquid extraction is a widely used technique of sample preparation in biomedical analysis. In spite of the high pre-concentration capacities of liquidliquid extraction, it suffers from a number of limitations including time and effort consumption, large organic solvent utilization, and poor performance in highly polar analytes. Homogeneous liquid-liquid extraction is an alternative sample preparation technique that overcomes some drawbacks of conventional liquid-liquid extraction, and allows employing greener organic solvents in sample treatment. In homogeneous liquid-liquid extraction, a homogeneous phase is formed between the aqueous sample and the water-miscible extractant, followed by chemically or physically induced phase separation. To form the homogeneous phase, aqueous samples are mixed with water-miscible organic solvents, waterimmiscible solvents/cosolvents, surfactants, or smart polymers. Then, phase separation is induced chemically (adding salt, sugar, or buffer) or physically (changing temperature or pH). This mode is rapid, sustainable, and cost-effective in comparison with other sample preparation techniques. Moreover, homogeneous liquid-liquid extraction is more suitable for the extraction of delicate macromolecules such as enzymes, hormones, and proteins and it is more compatible with liquid chromatography with tandem mass spectrometry, which is a vital technique in metabolomics and proteomics. In this review, the principle, types, applications, automation, and technical aspects of homogeneous liquid-liquid extraction are discussed.

show abstract

A fast and sensitive analytical procedure for monitoring of synthetic pyrethroid pesticides' metabolites in environmental water samples

Cited by 19 publications

References 36 publications

Rapid and sensitive analytical procedure for biomonitoring of organophosphate pesticide metabolites in human urine samples using a vortex‐assisted salt‐induced liquid–liquid microextraction technique coupled with ultra‐high‐performance liquid chromatography/tandem mass spectrometry

Rapid and sensitive analytical procedure for biomonitoring of organophosphate pesticide metabolites in human urine samples using a vortex‐assisted salt‐induced liquid–liquid microextraction technique coupled with ultra‐high‐performance liquid chromatography/tandem mass spectrometry

Determination of Three Typical Metabolites of Pyrethroid Pesticides in Tea Using a Modified QuEChERS Sample Preparation by Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry

Homogeneous liquid–liquid extraction as an alternative sample preparation technique for biomedical analysis

Contact Info

Product

Resources

About