A Fast and Simple Analysis of Glyphosate in Tea Beverages by Capillary Electrophoresis with On-Line Copper(II)-Glyphosate Complex Formation

Abstract

Glyphosate is an herbicide used for many plants that can be toxic to humans in high doses. Current methods for measuring glyphosate are slow and expensive. Here we describe a fast and simple method for measuring glyphosate in tea beverages by capillary electrophoresis with on-line formation of copper(II)-glyphosate complex. The optimum running conditions were found to be 40 mM acetate buffer (pH 5.0) containing 5 mM CuSO 4 with an effective voltage of +15 kV using a sulfonated capillary (FunCap-CE Type S) and direct UV detection at 250 nm. Linearity (r 2 > 0.999) was demonstrated in the range 5-1000 mg/l of glyphosate. Good reproducibilities of peak area (relative standard deviation < 1.2%) and migration time (relative standard deviation < 0.2%) were obtained. Recovery of glyphosate was between 98 and 100%. With this method, a tea beverage that was mixed with a glyphosate formulation was successfully analyzed.

One-step preparation of porous copper nanowires electrode for highly sensitive and stable amperometric detection of glyphosate

“…Kodama et al (2008) Liquid chromatography with electrochemical detection using a copper microelectrode.…”

A simple and rapid spectrophotometric method to quantify the herbicide glyphosate in aqueous media. Application to adsorption isotherms on soils and goethite

“…The development of analytical techniques for glyphosate quantification has increased in the last years and several quantification methods have been proposed (Byer et al, 2008;Kodama et al, 2008;Qian et al, 2009;Sánchez-Bayo et al, 2010;Songa et al, 2009). However, most of the analytical methods require expensive, specialized equipment and are out of economical reach for many laboratories.…”

Development of sampling for quantification of glyphosate in natural waters

“…The method involves the reaction of glyphosate with ninhydrin in the presence of molybdate and the product has maximum absorption at 570 nm. Kodama et al (2008) found glyphosate in tea beverages through the formation of the glyphosateCu(II) complex. The detection range was 5-1000 mg L -1 and recovery was from 98 to 100%.…”