A fast and validated capillary zone electrophoresis method for the determination of selected fatty acids applied to food and cosmetic purposes

Amorim, Tatiane Lima; Pena, Mariana Gavioli dos Reis; Costa, Fabiano Freire; Oliveira, Marcone Augusto Leal de; Chellini, Paula Rocha

doi:10.1039/c9ay01917k

Cited by 8 publications

(17 citation statements)

References 13 publications

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“…In some methods, the robust CZE-UV instrumental conditions applied in Sato's and Mimura's work were applied and validated to analyze other different vegetable oils. Quantification of C16:0, C18:0, C18:1 n-9, and C18:2 n-6 was performed in pumpkin oils [60]. In avocado, carthamus, evening primrose, and linseed oils, beyond these FA, C18:3 n-3 was also determined [61].…”

Section: Vegetable Oilsmentioning

confidence: 99%

Capillary electromigration methods for fatty acids determination in vegetable and marine oils: A review

et al. 2020

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Fatty acids determination is of paramount importance for quality control and suitable labeling of edible oils, required by regulatory agencies in several countries, and fast methods for this determination are worldly desired. This review article aimed to explore the available analytical methods for vegetable and marine oils analyses employing CE, which can be a straightforward and faster alternative than GC methods for fatty acid determination, considering some purposes. CE usually offers the possibility of a rapid analysis with a simple preparation of the sample, without requiring specific columns, which are inherent advantages of the technique. Instrumental conditions and the key points about fatty acids determination employing the technique are highlighted, and the main challenges and perspectives are also approached. Potential use of CE for edible oil analyses has been demonstrated for research and routine, which can be of interest for industries, regulatory agencies, and edible oil researchers. Therefore, we have explored the analytical approaches described in the last decades, intending to spread the interest of CE methods for fatty acid monitoring, label accuracy assessment, and food authenticity evaluation of edible oils.

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Section: Vegetable Oilsmentioning

confidence: 99%

Capillary electromigration methods for fatty acids determination in vegetable and marine oils: A review

et al. 2020

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“…Subsequently, the free fatty acids were methylated with 100 μL of BF3/methanol (14%) by heating for 10 minutes in a 60 °C water bath. The methylated fatty acids were extracted with 500 μL of hexane and analyzed 15 .…”

Section: Hydrolysis and Methylation Of Oilsmentioning

confidence: 99%

“…The data reported in the literature makes clear that fractions differ considerably in quantity from one raw material to another 15,23 . The content of certain fatty acids depend on the origin and specific cultivars.…”

Section: Fatty Acids Characterization and Tocopherol Contentmentioning

confidence: 99%

Pumpkin seeds (Cucurbita moschata - Jacarezinho cultivar): characterization of the oil extracted by solvent and supercritical fluid and study of anti-parasitary activity / Sementes de abóbora (Cucurbita moschata - cultivar Jacarezinho): caracterização do óleo extraído por solvente e fluido supercrítico e estudo da atividade antiparasitária

Chellini¹,

Gonçalves²,

Filho³

et al. 2022

BJDV

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The presence of many biologically active components makes pumpkins extremely attractive to the phytochemical manufacturing industry. Studies have demonstrated that the oil extracted from the seeds has different biological activities. This study aimed to determine the fatty acid composition and total tocopherol content of the pumpkin seed oil (Cucurbita moschata – Jacarezinho cultivar) extracted by supercritical carbon dioxide (Sc-CO2) and hexane/isopropanol. The fatty acids composition and content of total tocopherols were determined by GC, GC/FID, HPLC, respectively. We also evaluated in vitro schistosomicidal activities of the crude oil, which have been described with anti-parasitic activities. Sc-CO2 extracted pumpkin seed oil, with a maximum yield of 24.3 ± 0.4%, much higher than hexane/isopropanol extraction (8.3 ± 2.7%). Was not observe differences between the non-polar compounds present in the oil extracted by both methods. In the seed oil, unsaturated acids are dominant (oleic and linoleic). The results indicate that the oil has an excellent quality, with high contents of unsaturated fatty acids (73% of total fatty acids) and total tocopherols (14 mg100g-1 of oil). Although popularly reported as anti-parasitic activities caused no mortality, tegumental alterations or significant decrease in motor activity in all adult parasites. Sc-CO2 was able to extract pumpkin seed oil with a much higher yield than extraction with hexane/isopropanol and no differences were observe between the non-polar compounds present in the oil extracted by both methods. The oil is popularly used as an antiparasitic agent but in this study did not show antiparasitary actividies.

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“…Micellar electrokinetic chromatography (MEKC) can be used for the separation of FAs [ 6 , 7 , 8 ], including detection in real matrixes [ 9 , 10 , 11 ], as well as nonaqueous capillary electrophoresis [ 12 ]. In the latter case, the separation is performed using organic solvents (acetonitrile, methanol, and isopropanol) in the presence of aqueous ammonia or ammonium acetate.…”

Section: Introductionmentioning

confidence: 99%

Separation of unsaturated C18 fatty acids using perfluorinated‐micellar electrokinetic chromatography: I. Optimization and separation process

Perquis

Balayssac

et al. 2022

Electrophoresis

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Ammonium perfluorooctanoate (APFOA) was used as surfactant for the separation of free unsaturated C18 fatty acids by micellar electrokinetic chromatography. A simple background electrolyte of 50 mM APFOA water/methanol (90:10, v/v) at pH = 10 enabled repeatable separation of oleic acid, elaidic acid, linoleic acid, and alphalinolenic acid in less than 20 min. Separation conditions were optimized regarding various parameters (organic solvent, counterion, APFOA concentration, and pH).Because the repulsive interactions between fluorocarbon chains and hydrogenated chains are known to lead to segregation and phase separation, the choice of perfluorinated micelles to separate such perhydrogenated long-chain acids could appear astonishing. Therefore, the cmc, the charge density, and the mobility of the micelles have been determined, resulting in a first description of the separation process.

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A fast and validated capillary zone electrophoresis method for the determination of selected fatty acids applied to food and cosmetic purposes

Abstract: The potential of capillary electrophoresis was demonstrated to the determination of fatty acids in food and cosmetic samples and a validated method is proposed to palmitic, stearic, oleic and linoleic acids quantification in these samples.

Cited by 8 publications

References 13 publications

Capillary electromigration methods for fatty acids determination in vegetable and marine oils: A review

Capillary electromigration methods for fatty acids determination in vegetable and marine oils: A review

Separation of unsaturated C18 fatty acids using perfluorinated‐micellar electrokinetic chromatography: I. Optimization and separation process

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