A fluorescence ‘turn-on’ probe for the detection of thiols was prepared in three steps. A diaminoterephthalate chromophore (λabs = 472 nm) was equipped with a maleimide moiety as reactive group. Upon conjugate addition of the thiol, the fluorescence intensity is increased by a factor of about twenty (λem = 567 nm, quantum yield Φ = 1% → 14%). The probe is used to establish a quantitative assay for glutathione.