2015
DOI: 10.1039/c5ay00745c
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A fast high performance liquid chromatographic (HPLC) analysis of amino acid phenylketonuria disorder in dried blood spots and serum samples, employing C18 monolithic silica columns and photo diode array detection

Abstract: A gradient HPLC-PDA method applying a monolithic RP-C18 column for phenylalanine and tyrosine quantization in dried blood spots, within 6 min.

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Cited by 11 publications
(8 citation statements)
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“…Phenylalanine has been determined using chromatographic, , plasmonic, and fluorimetric techniques. , These methods involve specialized laboratory instrumentation and sometimes, complex extractions. As such, these methods are largely incompatible with point-of-care or at-home monitoring.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Phenylalanine has been determined using chromatographic, , plasmonic, and fluorimetric techniques. , These methods involve specialized laboratory instrumentation and sometimes, complex extractions. As such, these methods are largely incompatible with point-of-care or at-home monitoring.…”
Section: Resultsmentioning
confidence: 99%
“…Phenylalanine has been determined using chromatographic, 79,80 plasmonic, 81 and fluorimetric techniques. 79,82−84 These methods involve specialized laboratory instrumentation and sometimes, complex extractions.…”
Section: Acs Sensorsmentioning
confidence: 99%
“…Farideh Haghighi and others (Haghighi et al, 2015) performed the HPLC-PDA to detect two amino acids, namely phenylalanine and tyrosine, in DBS sample, and used Mass Spectroscopy; also, the time of their analysis was six minutes. In contrast, in our work, we detected amino acids using a fluorescence detector; furthermore, we avoided the presence of the interfering compounds due to the use of two chemical compounds: OPA and MPA reagents that only react with the amino group in the structure of the amino acids.…”
Section: Resultsmentioning
confidence: 99%
“…Chin Chen and others (Chin Chen et al, 2010) studied argininosuccinic aciduria disorder using ion-exchange chromatography with a dedicated amino acid analyzer (Biochrom 30) and post-column detection. Farideh Haghighi and others (Haghighi et al, 2015) determined phenylalanine and tyrosine concentration from Dried Blood Spot (DBS) using a Gradient High-Performance Liquid Chromatography with Photodiode Array Detection (HPLC-PDA). Tcherkas & Denisenko (Tcherkas & Denisenko, 2001) reported a method to measure homocysteine and cysteine as well as aspartate and glutamate level by Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC).…”
Section: Introductionmentioning
confidence: 99%
“…Most of methods commonly used for the determination of AAs, KAs, or FAs from biological material can also be applied for DBS samples, but the low quantity of analytes in the sample must be considered. AA levels in DBS samples are most often determined using HPLC with different types of detection—fluorescence (HPLC‐FLD) (Kanďár et al, 2009), photodiode array (HPLC‐PDA) (Haghighi et al, 2014), and HPLC‐electrospray ionization‐MS/MS (Bergwerff et al, 2016; Giordano et al, 2019), eventually using MS/MS without previous chromatographic separation (Wang et al, 2016). The methods for the determination of KA levels in DBS samples are quite rare, but as an example HPLC‐FLD (Kanďár et al, 2009) can be mentioned.…”
Section: Introductionmentioning
confidence: 99%