A fast highly sensitive colorimetric enzyme immunoassay system demonstrating benefits of enzyme amplification in clinical chemistry

Johannsson, A.; Stanley, Christopher J.; Self, Colin H.

doi:10.1016/0009-8981(85)90221-9

Cited by 92 publications

(29 citation statements)

References 4 publications

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“…Each of these measurements varied with body mass index, which tended to increase with birth weight and weight at 1 TABLE I-Mean weight, height, body mass index, waist to hip ratio, and systolic and diastolic blood pressures in men with and without impaired glucose tolerance or newly diagnosed diabetes as defined by plasma glucose concentrations two hours after 75 g oral load (15) 2 (4) 4 (14) 2 (10) 6 (13) 6 (6) 8 (7) 5 (9) (21) 24 (22) 14 (13) 6 (13) 3 (13) 4 (17) 7 (11) 8 (7) 5 (5) 3 (6) (18) 27 (7) 93 (25) *Original measurements were expressed in lb and were rounded.…”

Section: Methodsmentioning

confidence: 99%

Fetal and infant growth and impaired glucose tolerance at age 64.

Hales

Barker

Clark

et al. 1991

BMJ

2,472

1,533

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Objective-To discover whether reduced fetal and infant growth is associated with non-insulin dependent diabetes and impaired glucose tolerance in adult life.Design (0/24). Plasma glucose concentrations at 30 and 120 minutes fell with increasing birth weight and weight at 1 year. Plasma 32-33 split proinsulin concentration fell with increasing weight at 1 year. All these trends were significant and independent of current body mass. Blood pressure was inversely related to birth weight and strongly related to plasma glucose and 32-33 split proinsulin concentrations.Conclusions-Reduced growth in early life is strongly linked with impaired glucose tolerance and non-insulin dependent diabetes. Reduced early growth is also related to a raised plasma concentration of 32-33 split proinsulin, which is interpreted as a sign of 13 cell dysfunction. Reduced intrauterine growth is linked with high blood pressure, which may explain the association between hypertension and impaired glucose tolerance.

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Section: Methodsmentioning

confidence: 99%

Fetal and infant growth and impaired glucose tolerance at age 64.

Hales

Barker

Clark

et al. 1991

BMJ

2,472

1,533

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“…Antibodies to S. typhimurium aroA and C . trachomatis serovar B were detected by redox-amplified ELISA (Johannsson et al, 1985). S. typhimurium aroA or C. trachomatis serovar B whole cells at a protein concentration of 10 pg ml-1 were coupled to microtitre PVC microtitre trays (Flow laboratories) by overnight incubation in carbonate buffer at 18 "C (1.6 g 1-l Na2C03, 2.95 g 1-l NaHCO,, 0.2 g 1-l NaN,; pH 9.6).…”

Section: Methodsmentioning

confidence: 99%

Chlamydia trachomatis major outer membrane protein epitopes expressed as fusions with LamB in an attenuated aroA strain of Salmonella typhimurium; their application as potential immunogens

Hayes

Conlan²,

Everson³

et al. 1991

Journal of General Microbiology

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The major outer-membrane protein (MOMP) of Chlamydia trachomatis is the focus of attention for chlamydial vaccine design, particularly those serovar-and subspecies-specific epitopes which provoke neutralizing immune responses. Selected surface-exposed B-cell epitopes of MOMP, incorporating B-subspecies specificities, were expressed as fusions with LamB, an inducible outer-membrane transport protein of Escherichia culi. These recombinant chlamydial-LamB proteins were correctly transported to the outer membrane of both E. culi and an aruA mutant of Salmonella typhimurium. The immunogenicity of the constructs was investigated in a mouse model of chlamydial salpingitis. After oral immunization, recombinant S. typhimurium were recovered from the livers of mice for up to two weeks, and a serum IgG response was induced both to the Salmunella and to the inserted chlamydial epitopes. By contrast, intravenous inoculation was ineffective. Although these LamB fusions proved only weakly immunogenic, this approach should be useful for investigating the ability of attenuated S. typhimurium vaccines incorporating chlamydial epitopes to stimulate protective mucosal immunity in the mouse model of chlamydial salpingitis.

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“…In the insulin and proinsulin assays the alkaline phosphatase activity was measured using the amplification system (8,9), with the change in absorbance at 490 nm measured continuously.…”

Section: Measurement Of Enzyme Activitymentioning

confidence: 99%

“…In this system -nicotinamide adenine dinucleotide phosphate, reduced form (NADPH) was first converted to -nicotinamide adenine dinucleotide (NADH) by alkaline phosphatase. The NADH was then used as a cofactor in a cyclic reaction involving the enzymes diaphorase and alcohol dehydrogenase to alternately oxidize and reduce the cofactor in parallel with the reduction of a dye (8,9).…”

Section: Introductionmentioning

confidence: 99%

Sensitive Amplified Immunoenzymometric Assays (IEMA) for Human Insulin and Intact Proinsulin

Alpha

Cox²,

Crowther³

et al. 1992

Clinical Chemistry and Laboratory Medicine

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Summary:Immunoenzymometric assays (lEMAs) for human insulin and intact proinsulin were developed using the amplification system developed by Johannsson et al. (Clin. Chim. Acta 148 (1985) 119 -124) for the detection of the enzyme alkaline phosphatase. The detection limit of the assays was 0.8 pmol/1 for proinsulin and 0.8 pmol/1 for insulin whereas it was 1.8 pmol/1 and 2.3 pmol/1 respectively for the homologous immunoradiometric assays (IRMA). These assays are superior to immunoradiometric assays in terms of sensitivity, shelf-life of the labelled antibody and suitability for automation.

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A fast highly sensitive colorimetric enzyme immunoassay system demonstrating benefits of enzyme amplification in clinical chemistry

Cited by 92 publications

References 4 publications

Fetal and infant growth and impaired glucose tolerance at age 64.

Fetal and infant growth and impaired glucose tolerance at age 64.

Chlamydia trachomatis major outer membrane protein epitopes expressed as fusions with LamB in an attenuated aroA strain of Salmonella typhimurium; their application as potential immunogens

Sensitive Amplified Immunoenzymometric Assays (IEMA) for Human Insulin and Intact Proinsulin

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