A Fast Iterative Digital Volume Correlation Algorithm for Large Deformations

Bar-Kochba, Eyal; Toyjanova, Jennet; Andrews, Edward E.; Kim, K.-S.; Franck, Christian

doi:10.1007/s11340-014-9874-2

Cited by 208 publications

(176 citation statements)

References 42 publications

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“…Briefly, three-dimensional time-lapsed volumetric images of fluorescent beads embedded in polyacrylamide substrates were recorded using laser scanning confocal microscopy. A new fast iterative digital volume correlation algorithm was used to track the motion of the embedded fluorescent beads in all three dimensions between time increments (20). This newly developed fast iterative digital volume correlation technique provides significantly higher spatial resolution, signal/noise, and faster computation times than our previous digital volume correlation algorithm (21).…”

Section: Methodsmentioning

confidence: 99%

“…The quantity B ϭ F⅐F T is the left Cauchy-Green tensor, and J is the Jacobian of deformation gradient tensor F. In both of these relations, the deformation gradient tensor F is calculated from the full-field displacements using an 11-tap discrete differentiation kernel described by Farid et al (23), which we showed to provide optimal spatial accuracy in the presence of noise (20).…”

Section: Methodsmentioning

confidence: 99%

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Matrix Confinement Plays a Pivotal Role in Regulating Neutrophil-generated Tractions, Speed, and Integrin Utilization

Toyjanova

Flores-Cortez

Reichner

et al. 2015

Journal of Biological Chemistry

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Background: Neutrophils must generate well regulated forces to migrate within the confined, three-dimensional space of diseased tissues. Results: Spatial confinement induces a switch to integrin-independent motility, but integrins regulate three-dimensional traction force generation. Conclusion: Physical confinement is sufficient to induce integrin-independent motility. Significance: Spatially confined double hydrogels are a biomimetic system that may allow for ex vivo testing of neutrophilspecific therapeutics.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Matrix Confinement Plays a Pivotal Role in Regulating Neutrophil-generated Tractions, Speed, and Integrin Utilization

Toyjanova

Flores-Cortez

Reichner

et al. 2015

Journal of Biological Chemistry

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“…Briefly, three-dimensional timelapsed volumetric images of fluorescent beads embedded in PA substrates were recorded using laser scanning confocal microscopy (LSCM). A new fast iterative digital volume correlation (FIDVC) algorithm was used to track the motion of the embedded fluorescent beads in all three dimensions between each time increment [22]. Owing to the spatial refinement of the subvolume correlation windows, the FIDVC is capable of capturing large nonlinear displacement fields with high spatial resolution while maintaining low computation times (%1.5 min per time increment for a typical image size of 512 Â 512 Â 160 voxels).…”

Section: Three-dimensional Traction Force Microscopymentioning

confidence: 99%

“…Cultured cells were maintained in a humidified chamber at 378C with 5% CO 2 . Cells were trypsinized using 0.25% trypsin and seeded in SC medium on functionalized gels at a density of 950 SCs cm 22 for 3 h to allow for cellular attachment. Cell-labelling solution, Vybrant DiD (far red, 30 ml ml 21 of SC medium) was applied to each sample for 30 min at 378C and 5% CO 2 followed by three rinses with medium for 10 min at 378C and 5% CO 2 .…”

Section: Cell Culturementioning

confidence: 99%

Three-dimensional traction forces of Schwann cells on compliant substrates

López-Fagundo

Bar-Kochba

Livi

et al. 2014

J. R. Soc. Interface.

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The mechanical interaction between Schwann cells (SCs) and their microenvironment is crucial for the development, maintenance and repair of the peripheral nervous system. In this paper, we present a detailed investigation on the mechanosensitivity of SCs across a physiologically relevant substrate stiffness range. Contrary to many other cell types, we find that the SC spreading area and cytoskeletal actin architecture were relatively insensitive to substrate stiffness with pronounced stress fibre formation across all moduli tested (0.24-4.80 kPa). Consistent with the presence of stress fibres, we found that SCs generated large surface tractions on stiff substrates and large, finite material deformations on soft substrates. When quantifying the three-dimensional characteristics of the SC traction profiles, we observed a significant contribution from the out-of-plane traction component, locally giving rise to rotational moments similar to those observed in mesenchymal embryonic fibroblasts. Taken together, these measurements provide the first set of quantitative biophysical metrics of how SCs interact with their physical microenvironment, which are anticipated to aid in the development of tissue engineering scaffolds designed to promote functional integration of SCs into post-injury in vivo environments.

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“…3. For calculating cell force generation and deformation, generate a computational code to find the displacements of the fluorescence beads following the TFM/DVC protocols 31 .…”

Section: Tracking Cell Migration Utilizing Tfmmentioning

confidence: 99%

Planar Gradient Diffusion System to Investigate Chemotaxis in a 3D Collagen Matrix

Stout

Toyjanova²,

Franck

2015

JoVE

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The importance of cell migration can be seen through the development of human life. When cells migrate, they generate forces and transfer these forces to their surrounding area, leading to cell movement and migration. In order to understand the mechanisms that can alter and/or affect cell migration, one can study these forces. In theory, understanding the fundamental mechanisms and forces underlying cell migration holds the promise of effective approaches for treating diseases and promoting cellular transplantation. Unfortunately, modern chemotaxis chambers that have been developed are usually restricted to two dimensions (2D) and have complex diffusion gradients that make the experiment difficult to interpret. To this end, we have developed, and describe in this paper, a direct-viewing chamber for chemotaxis studies, which allows one to overcome modern chemotaxis chamber obstacles able to measure cell forces and specific concentration within the chamber in a 3D environment to study cell 3D migration. More compelling, this approach allows one to successfully model diffusion through 3D collagen matrices and calculate the coefficient of diffusion of a chemoattractant through multiple different concentrations of collagen, while keeping the system simple and user friendly for traction force microscopy (TFM) and digital volume correlation (DVC) analysis. Video LinkThe video component of this article can be found at

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A Fast Iterative Digital Volume Correlation Algorithm for Large Deformations

Cited by 208 publications

References 42 publications

Matrix Confinement Plays a Pivotal Role in Regulating Neutrophil-generated Tractions, Speed, and Integrin Utilization

Matrix Confinement Plays a Pivotal Role in Regulating Neutrophil-generated Tractions, Speed, and Integrin Utilization

Three-dimensional traction forces of Schwann cells on compliant substrates

Planar Gradient Diffusion System to Investigate Chemotaxis in a 3D Collagen Matrix

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